SMAS fold flap and ADM repair of the parotid bed following removal of parotid haemangiomas via pre- and retroauricular incisions to improve cosmetic outcome and prevent Frey's syndrome.

The growth of parotid haemangiomas during the proliferative phase may be rapid and unpredictable. Involution often takes many years, with attendant psychological sequelae to the child. Although conservative management is usually proposed for parotid haemangiomas occurring in infancy, this may not be particularly helpful and the haemangioma difficult to conceal.

Cryopreservation of human ovarian tissue by solid-surface vitrification.

Objective: To cryopreserve human ovarian tissue using solid-surface vitrification (SSV) technique for the first time.

Study Design: Human ovarian slices from each of 26 patients were randomly allocated to fresh, SSV, and slow-freezing groups, respectively. Histological observation and the TUNEL assay of the tissue were performed after cryopreservation.

Endogenous pro-resolving and anti-inflammatory lipid mediators: the new hope of atherosclerotic diseases.

Atherosclerosis is a complex disease process in which genetic, lipid, cellular, and immunological factors combine to determine the location, severity, and timing of lesion development and clinical events. It has been demonstrated, however, that inflammation governed atherosclerosis during the course of development of atherosclerosis. It has also been demonstrated to be effective to decrease the cardiovascular events and improve the prognosis of atherosclerotic diseases by regulating inflammatory reaction (e.

Severe liver disease in pregnancy.

Objective: To determine the clinical characteristics of patients with fulminant hepatitis of pregnancy (FHP) and acute fatty liver of pregnancy (AFLP) and analyze their correlation with pregnancy outcome.

Methods: Of 55 pregnant women with severe liver disease, 41 had FHP and 14 had AFLP.

Results: Jaundice was the primary manifestation for both FHP and AFLP and hepatic encephalopathy was the most significant complication for both.

Pedicled mandibular osteomuscular flap for zygomatic reconstruction through a modified Weber-Ferguson incision.

The aim of this study was to evaluate the use of a pedicled mandibular osteomuscular flap for zygoma reconstruction through a modified Weber-Ferguson incision. The study included 3 male patients and 2 female patients with an average age of 46 years. Zygomatic ameloblastoma was present in 2 cases.

[Risk stratification and prognostic evaluation of patients with pulmonary thromboembolism].

Objective: To explore the risk stratification and prognostic evaluation of pulmonary thromboembolism (PTE).

Methods: The clinical data of 46 patients suffering from PTE diagnosed by ventilation perfusion scan or spiral CT pulmonary angiography admitted to our hospital from January 2002 to December 2006 were analyzed retrospectively.

Results: The total mortality was 33% (15/46 cases).

[Influence of iNOS silencing by RNA interference on proliferation activity of Tca8113 cell].

Objective: To investigate the apoptosis and proliferation activity of Tca8113 cells.

Methods: The vector that involves short hairpin RNA of iNOS was transfected to Tca8113 cells. The change of iNOS expression was observed using immunohistochemistry technique, the apoptosis rate examined by flow cytometry, and the proliferation Tca8113 cells examined by methyl thiazolyl tetrazolium (MTT).

Bmi-1 expression predicts prognosis for patients with gastric carcinoma.

Background And Objective: The Bmi-1 gene is a transcriptional repressor involved in oncogenesis in various human cancers. Here, we examine Bmi-1 expression in gastric carcinoma (GC) and investigates whether its expression correlates with patient prognosis.

Methods: Immunohistochemistry was performed using an anti-Bmi-1 antibody on primary tumor samples of 146 cases of GC.

Selective enrichment of hepatocytes from mouse embryonic stem cells with a culture system containing cholestatic serum.

Aim: There is increasing evidence indicating that embryonic stem (ES) cells are capable of differentiating into hepatocyte-like cells in vitro. However, it is necessary to improve the differentiation efficiency so as to promote the clinical application. Here, we report an efficient culture system to support hepatocyte differentiation from ES cells by utilizing cholestatic serum.

A pilot study on combination compartmentalisation and sclerotherapy for the treatment of massive venous malformations of the face and neck.

Venous malformations of the face and neck involve multiple anatomical spaces and encase critical neuromuscular structures, making surgical treatment difficult; high recurrence rates and high morbidity are well documented. The purpose of this study was to evaluate the clinical curative effect of combination compartmentalisation and sclerotherapy for the treatment of massive venous malformations of the face and neck. Sixteen patients with massive venous malformations of the face and neck region (12 males and four females; mean age: 14.

[Comparative study on gasless endoscopic versus conventional thyroid surgery].

Objective: To evaluate the efficiency, feasibility and safety of gasless endoscopic thyroid surgery through compared research.

Methods: From July 2001 to Oct. 2005, 83 cases that had received gasless endoscopic thyroid surgery were compared with 80 cases that had received conventional surgery and analyzed on operating time, blood loss, cosmetic effects, complications and costs.

IL-1beta sensitizes rat intervertebral disc cells to Fas ligand mediated apoptosis in vitro.

Aim: To determine the apoptotic effect of recombinant rat Fas Ligand on rat intervertebral disc cells pre-treated with IL-1beta in vitro, and the expression of Fas in cultured rat intervertebral disc cells.

Methods: Cells were isolated from the inner annulus fibrosus and transition zones of lumbar discs from Sprague-Dawley rats. The cells were grown in monolayer and divided in 5 treatment groups.

[Relationship between childhood obstructive sleep apnea-hypopnea syndrome and tympanogram].

Objective: To explore the correlation between childhood obstructive sleep apnea-hypopnea syndrome and tympanogram.

Method: A prospective clinical study was carried out. Polysomnography and tympanogram were analyzed in 72 cases.

Pedicle screw fixation of the cervical spine: guidance by computed tomography.

This is an anatomic, preclinical study using computed tomography and computerized simulation to describe the morphologic features and ideal screw insertion trajectories for cervical pedicles in the southern Chinese population. The morphologic features of the pedicle of vertebrae (C3-C7) from 20 human cadaver spines were evaluated with computed tomography and with manual measurements. There was no difference between the two data sets.

[Evaluation of effectiveness of advanced tonsillar carcinoma by different treatment].

Objective: To evaluate the treatment of advanced tonsillar carcinoma by radiotherapy plus salvage surgery (R+S) or surgery coupling with postoperative radiotherapy (S+R).

Method: Clinical data of 48 patients with advanced tonsillar carcinoma who were treated in The 2nd Affiliated Hospital of Sun Yat-sen University from June 1996 to June 2004 was retrospectively analyzed. The patients were divided into R+S group (group A, 21 cases) and S+R group (group B, 27 cases).

[Difference in ex vivo expansion of megakaryocytes derived from umbilical cord blood and peripheral blood].

Objective: Currently, thrombocytopenia is typically observed in patients undergoing hematopioetic stem cell transplantation (HSCT), high-dose chemotherapy or irradiation. Severe thrombocytopenia can cause intestinal and intracranial hemorrhage. To transfuse ex vivo-expanded megakaryocytes (MK) into patients can reinforce the ability of platelet formation and shorten the time of platelet recovery.

[Effect of peroxisome proliferator-activated receptor-alpha agonist on adipokines expression in rats fed with high-fat diet].

Objective: To explore the effect of peroxisome proliferator-activated receptor-alpha ( PPAR-alpha) agonist fenofibrate on adipokines expression in high-fat diet fed SD rats and its relationship to insulin resistance (IR).

Methods: Rats were randomized into three groups (n = 10) : HD group, fed with high-fat diet; HDF group, fed with high fat diet and treated with fenofibrate; and control group, fed with normal diet. Animals were sacrificed after 4-week follow-up.

Reconstruction of full-thickness cheek defects with combined temporalis myofacial and facial-cervico-pectoral flaps.

Objective: The objective of this study was to assess using the temporal myofacial flaps (TMFF) and the facial-cervico-pectoral flap (FCPF) to provide both inner and outer linings for large full-thickness cheek defects following ablative oral cancer surgery.

Study Design: Twelve patients with malignant tumors in the buccal region were treated by extensive surgical dissection, and the cheek mucosa defects were repaired with the TMFF and the cheek skin defects were reconstructed with the FCPF. There were 9 male and 3 female patients, age range from 18 to 70 years (mean 52.

Extended vertical lower trapezius island myocutaneous flap for reconstruction of cranio-maxillofacial defects.

This study explored an effective method for repairing cranio-maxillofacial soft-tissue defects following radical craniofacial surgery in four patients with malignant tumours involving the skull base and frontal region. The large cranio-maxillofacial soft-tissue defects were reconstructed using an extended vertical lower trapezius island myocutaneous flap based on the transverse cervical artery. The flap was 8-12 cm long and 5-7 cm wide.

[Diagnosis of recurrent or residual nasopharyngeal carcinomas in the skull base area with F-18-fluoro-deoxyglucose positron emission tomography].

Objective: To investigate the diagnostic value of F-18-fluoro-deoxyglucose positron emission tomography (FDG-PET) for the recurrent or residual nasopharyngeal carcinomas in the skull base area.

Methods: Nine post-irradiation nasopharyngeal carcinoma patients did FDG-PET scanning, CT/MRI imaging and underwent nasopharynx and skull base-biopsy under endoscopy. The results of FDG-PET were evaluated and compared with CT/MRI studies and biopsies.

[Effect of ulinastatin on apoptosis in ileal mucosa of rats with hemorrhagic shock].

Objective: To investigate the effect of ulinastatin on apoptosis in ileal mucosa of rats with hemorrhagic shock.

Methods: A prospective, controlled animal study was performed. The rat model of hemorrhagic shock was replicated according to method described by Chaudry.

In vitro differentiation of mouse ES cells into hepatocytes with coagulation factors VIII and IX expression profiles.

Coagulation factors II, V, VII, VIII, IX and X are produced by hepatocytes. So factors VIII and IX deficiencies, which result in hemophilia A and B, have the potential to respond to cellular replacement therapy. Embryonic stem (ES) cells provide a unique source for therapeutic applications.

Abnormal glucose tolerance in Chinese women with polycystic ovary syndrome.

Background: The aims of this study were to analyse the prevalence of impaired glucose tolerance (IGT) and diabetes mellitus (NIDDM) in Chinese polycystic ovary syndrome (PCOS) patients and to assess the ability of screening tests to predict these abnormalities within this population.

Methods: A total of 102 PCOS patients were evaluated. All patients underwent oral glucose tolerance tests (OGTTs) with blood samples taken at 0, 1 and 2 h.

[The risk factors and preventive strategies of heterotopic ossification after artificial disc replacement in lumbar spine].

Objective: To analyze the risk factors of heterotopic (HO) ossification after total lumbar disc replacement and probe the preventive strategies for it.

Methods: The radiographs and clinical data of 78 discs in 65 patients who received artificial lumbar disc replacement (ADR) from April 1998 to December 2003 were analyzed retrospectively by two radiologists and one orthopaedic surgeon and then postoperative HO were graded according to McAfee system. The bony formations in disc spaces, time of HO were found, and range of motion (ROM) of the operated levels were measured on radiographic films.