Asperosaponin VI inhibition of DNMT alleviates GPX4 suppression-mediated osteoblast ferroptosis and diabetic osteoporosis.

Introduction: Diabetic osteoporosis (DOP) is an insidious complication of diabetes with limited therapeutic options. DOP is pathologically associated with various types of regulated cell death, but the precise role of ferroptosis in the process remains poorly understood. Asperosaponin VI (AVI), known for its clinical efficacy in treating bone fractures and osteoporosis, may exert its osteoprotective effects through mechanisms involving ferroptosis, however this has not been established.

Construction and characterization of a novel secreted MsC-CAR-T cell in solid tumors.

The CD47-SIRPα signaling has been acknowledged as a significant immune checkpoint and CD47 blocking has been proved as a potential therapeutic strategy for the treatment of solid tumor. However, the potential application of CAR-T cells secreted antibody fragment simultaneously in solid tumor is rarely explored. In this study, we searched bioinformatic databases and investigated the characteristics of CD47 in solid tumors.

Mucosal Hub Bacteria as Potential Targets for Improving High-Fat Diet-Related Intestinal Barrier Injury.

Intestinal barrier injury contributes to multiple diseases such as obesity and diabetes, whereas no treatment options are available. Due to close interactions between mucosal microbiome and intestinal barrier, we evaluated the potential of mucosal bacteria in providing targets for high-fat diet (HFD)-related intestinal barrier injury. Whole-genome metagenomics was used to evaluate mucosal microbiome, while intestinal barrier injury was estimated using serum LPS, FITC-dextran intensity, and ZO-1 protein.

Dynamic Diselenide Hydrogels for Controlled Tumor Organoid Culture and Dendritic Cell Vaccination.

Dynamic hydrogels are emerging as advanced materials for engineering tissue-like environments that mimic cellular microenvironments. We introduce a diselenide-cross-linked hydrogel system with light-responsive properties, designed for precise control of tumor organoid growth and light-initiated radical inactivation, particularly for dendritic cell (DC) vaccines. Diselenide exchange enables stress relaxation and hydrogel remodeling, while recombination and quenching of seleno radicals (Se) reduce cross-linking density, leading to controlled degradation.

Safety and efficacy of intravenous recombinant human prourokinase for acute ischaemic stroke within 4·5 h after stroke onset (PROST-2): a phase 3, open-label, non-inferiority, randomised controlled trial.

Background: Intra-arterial prourokinase has been shown to be a promising thrombolytic agent in patients with acute ischaemic stroke. Given the global shortage of thrombolytics, we aimed to assess the non-inferiority of intravenous recombinant human prourokinase compared with alteplase in patients with acute ischaemic stroke who were ineligible for or who refused endovascular thrombectomy.

Methods: PROST-2 was a phase 3, open-label, non-inferiority, randomised controlled trial conducted at 61 hospitals in China.

IGFBP5 affects cardiomyocyte survival and functional recovery in mice following myocardial ischemia.

Insulin-like growth factor-binding protein 5 (IGFBP5) has been shown to be useful for the diagnosis and treatment of multiple tumors and cerebrovascular diseases. However, it is unknown whether IGFBP5 is involved in myocardial repair following myocardial infarction (MI). Here we show high expression of IGFBP5 in multiple models of ischemic and hypoxic injury.

CT-based whole lung radiomics nomogram to identify middle-aged and elderly COVID-19 patients at high risk of progressing to critical disease.

Background: COVID-19 remains widespread and poses a threat to people's physical and mental health, especially middle-aged and elderly individuals. Early identification of COVID-19 patients at high risk of progressing to critical disease helps improve overall patient outcomes and healthcare efficiency.

Purpose: To develop a radiomics nomogram to predict the risk of newly admitted middle-aged and elderly COVID-19 patients progressing to critical disease.

Pharmacokinetics of infigratinib and its active metabolites in Chinese patients with advanced gastric cancer harboring FGFR2 gene amplification.

Infigratinib, an FGFR1-3 selective oral tyrosine kinase inhibitor, has shown clinical activity in cancers with FGFR alterations. The pharmacokinetics (PK) of infigratinib and its major metabolites have been characterized in global populations. This study examined the PK profile of infigratinib and its metabolites in Chinese patients.

Redox-sensitive epigenetic activation of SUV39H1 contributes to liver ischemia-reperfusion injury.

Liver ischemia-reperfusion (I/R) injury is a clinically relevant pathophysiological process that determines the effectiveness of life-saving liver transplantation, to which aberrant ROS accumulation plays a key role. In the present study we investigated the role of SUV39H1, a lysine methyltransferases, in this process focusing on regulatory mechanism and translational potential. We report that SUV39H1 expression was up-regulated in the liver tissues of mice subjected to ischemia-reperfusion and in hepatocytes exposed to hypoxia-reoxygenation (H/R) in a redox-sensitive manner.

A Near-Infrared Imaging System for Robotic Venous Blood Collection.

Venous blood collection is a widely used medical diagnostic technique, and with rapid advancements in robotics, robotic venous blood collection has the potential to replace traditional manual methods. The success of this robotic approach is heavily dependent on the quality of vein imaging. In this paper, we develop a vein imaging device based on the simulation analysis of vein imaging parameters and propose a U-Net+ResNet18 neural network for vein image segmentation.

Suspension and suturing technique can reduce the incidence of anastomotic leakage after rectal cancer excision: a single, prospective, cohort study.

To evaluate the value of suspension and suturing technique in the prevention of anastomotic leakage of rectal cancer following double stapling technique. We performed a cohort study between August 2018 and December 2021 and analyzed the patients with rectal cancer underwent radical resection and double stapling technique at the department of general surgery. 324 patients were recruited in this research.

CoronaVac-induced antibodies that facilitate Fc-mediated neutrophil phagocytosis track with COVID-19 disease resolution.

Although severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants raise concerns about decreased vaccine efficacy, vaccines continue to confer robust protection in humans, implying that immunity beyond neutralization contributes to vaccine efficacy. In addition to neutralization, antibodies can mediate various Fc-dependent effector functions, including antibody-dependent cellular phagocytosis (ADCP), antibody-dependent neutrophil phagocytosis (ADNP) and antibody-dependent cellular cytotoxicity (ADCC). However, the specific role of each Fc-mediated effector function in contributing to COVID-19 disease attenuation in human remains unclear.

QTc interval prolongation and risk of atrial fibrillation recurrence: a meta-analysis and observational cohort study.

Introduction: Corrected QT interval (QTc) is a ventricular repolarization marker on electrocardiography. Previous studies evaluated its value in predicting atrial fibrillation (AF) occurrence. However, its predictive efficacy for AF recurrence remains controversial.

Advantages and rational application of indocyanine green fluorescence in pulmonary nodule surgery: a narrative review.

Background And Objective: The early detection and early treatment of high-risk pulmonary nodules directly affect the long-term survival rate of patients. However, conventional nodule localization methods, such as hook-wire, technetium-99m, and methylene blue are associated with issues such as a high-frequency of complications, low patient tolerance, serious side effects, and inability to identify pigmented lungs. For patients who require segmentectomy, there is often a lack of effective path planning, resulting in insufficient resection margins or excessive loss of lung function.

Safety and efficacy of a novel quantitative phase I exercise in patients after cardiopulmonary bypass: a single-blind, prospective, non-randomized study.

Background: Despite the positive effect of early post-cardiac surgery exercises, the concern of safety issues is high, indicating the need for standardized and quantitative mobility indicators to enhance early mobilization monitoring in the intensive care unit while ensuring patient safety. This study aimed to compare the safety and efficacy of quantitative phase I exercise versus usual care in patients who have undergone cardiac surgery.

Methods: In this non-randomized, single-blind trial, patients following on-pump cardiac surgery were allocated to either quantitative exercise (intervention group, n=114) or usual care (control group, n=114) based on their willingness.

Hydrogen sulfide-generating semiconducting polymer nanoparticles for amplified radiodynamic-ferroptosis therapy of orthotopic glioblastoma.

A variety of therapeutic strategies are available to treat glioblastoma (GBM), but the tumor remains one of the deadliest due to its aggressive invasiveness, restrictive blood-brain barrier (BBB), and exceptional resistance to drugs. In this study, we present a hydrogen sulfide (HS)-generating semiconducting polymer nanoparticle (PFeD@Ang) for amplified radiodynamic-ferroptosis therapy of orthotopic glioblastoma. Our results show that in an acidic tumor microenvironment (TME), HS donors produce large amounts of HS, which inhibits mitochondrial respiration and alleviates cellular hypoxia, thus enhancing the radiodynamic effect during X-ray irradiation; meanwhile, Fe is reduced to Fe by tannic acid in an acidic TME, which promotes an iron-dependent cell death process in tumors.

Multifunctional Microneedle Patches for Perivascular Gene Delivery and Treatment of Vascular Intimal Hyperplasia.

Gene therapy has emerged as a promising approach to address challenging cardiovascular diseases. Extensive efforts have been focused on developing highly efficient gene vectors with precise delivery techniques to enhance its effectiveness. In this study, we present multifunctional dopamine-gelatin microneedle patches with gene therapy capabilities to achieve perivascular gene delivery for intimal hyperplasia treatment.