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The use of the modified hypo-osmotic swelling test for the selection of immotile testicular spermatozoa in patients treated with ICSI: a randomized controlled study.
Hum Reprod
December 2005
Departments of Obstetrics and Gynaecology, and Andrology, the University of Alexandria and the Alexandria Fertility Centre, Alexandria, Egypt.
Background: Various methods for the selection of viable sperm from among immotile testicular spermatozoa for use in ICSI have been described in non-randomized studies. We have conducted a randomized controlled study to compare the use of the modified hypo-osmotic swelling (HOS) test (50% culture medium + 50% Milli-Q grade water) with that of sperm selection on the basis of their morphology alone.
Methods: A total of 79 couples with immotile testicular spermatozoa treated with ICSI were randomly assigned into two groups.