Downregulation of N6-Methyladenosine (m6A) Methylation of Sema4D mRNA Contributes to Treg Dysfunction and Allograft Rejection.

Regulatory T cells (Tregs) has been shown to be involved in the induction of transplantation tolerance in numerous models. Our previous work demonstrated that METTL14 loss impaired Treg function and hindered the establishment of transplantation tolerance. However, the underlying mechanisms remain unclear.

Harnessing natural saponins: Advancements in mitochondrial dysfunction and therapeutic applications.

Background: Mitochondrial dysfunction plays a crucial role in the development of a variety of diseases, notably neurodegenerative disorders, cardiovascular diseases, metabolic syndrome, and cancer. Natural saponins, which are intricate glycosides characterized by steroidal or triterpenoid structures, have attracted interest due to their diverse pharmacological benefits, including anti-inflammatory, antiviral, and anti-aging effects.

Purpose: This review synthesizes recent advancements in understanding mitochondrial dysfunction and explores how saponins can modulate mitochondrial function.

Efficacy of radiotherapy in treating local recurrence concomitant with distant metastasis of nasopharyngeal carcinoma: a long-term retrospective multicenter study.

Purpose: Patients with nasopharyngeal carcinoma (NPC) experiencing locoregional recurrence concomitant with distant metastases (rmNPC) after initial treatment represent a unique subgroup with significant management challenges. This study aimed to evaluate overall survival (OS) in rmNPC patients treated with systemic therapies with or without radiotherapy.

Methods: This retrospective multicenter study included patients with locally recurrent and metastatic NPC from five hospitals.

Melatonin lessens the susceptibility to atrial fibrillation in sleep deprivation by ameliorating Ca mishandling in response to mitochondrial oxidative stress.

Background: The antiarrhythmic effect of melatonin(MLT) has been demonstrated in several studies; however, this hypothesis has recently been contested. Our research seeks to determine if exogenous MLT supplementation can reduce atrial fibrillation (AF) susceptibility due to sleep deprivation (SD) by addressing Ca mishandling and atrial mitochondrial oxidative stress.

Methods: Adult rats received daily MLT or vehicle injections and were exposed to a modified water tank.

Cognition and influencing factors of secondary prevention in patients with ischemic stroke 1 year after discharge in Southwest China: a cross-sectional survey.

Although the risk of recurrent stroke is very high in patients with ischemic stroke (IS), the implementation of secondary prevention of IS has not received enough attention. Therefore, we aimed to investigate the cognition and compliance status of secondary prevention in patients with IS in southwest China and explore the factors affecting compliance with secondary prevention 1 year after discharge. We conducted a cross-sectional survey of patients with IS 1 year after discharge in southwest China through convenience sampling.

Exploring the Mechanism of Acupuncture in Improving Ovarian Function in Rats with Poor Ovarian Response Using High-Throughput Sequencing.

Objective: This study aimed to investigate the possible mechanism through which acupuncture protects ovaries with Poor Ovarian Response (POR) in rats based on microRNA (miRNA).

Methods: Thirty-six SPF SD female non-pregnant rats aged 8 weeks were randomly divided into the blank group, model group, and acupuncture group, with 12 rats in each group. According to the group, the rats were given gavage of Tripterygium wilfordii polyglycosides suspension for 14 days to establish the model of POR, and then the rats were treated with acupuncture for 2 weeks, once a day, for 20 minutes.

Regulating astrocyte phenotype by Lcn2 inhibition toward ischemic stroke therapy.

Astrocytes can be reacted to "reactive astrocytes" after ischemia-reperfusion injury, in which A1 phenotype causes neuronal and oligodendrocyte death, whereas the A2 phenotype exerts neuroprotective effects, thus regulating reactive astrocyte to A2 type is a potential target for stroke therapy. Lcn2 level is highly associated with the phenotypic polarization of astrocytes. We found that silencing the Lcn2 gene by adeno-associated virus (AAV)-Lcn2 shRNA adenovirus resulted in a dramatic decrease in A1-type astrocytes and increase in A2 astrocytes in MCAO mice.

Deep learning-based MVIT-MLKA model for accurate classification of pancreatic lesions: a multicenter retrospective cohort study.

Background: Accurate differentiation between benign and malignant pancreatic lesions is critical for effective patient management. This study aimed to develop and validate a novel deep learning network using baseline computed tomography (CT) images to predict the classification of pancreatic lesions.

Methods: This retrospective study included 864 patients (422 men, 442 women) with confirmed histopathological results across three medical centers, forming a training cohort, internal testing cohort, and external validation cohort.

Association between METTL14 gene polymorphisms and risk of ovarian endometriosis.

Background: Endometriosis, a prevalent chronic gynecological condition, is frequently associated with infertility and pelvic pain. Despite numerous studies indicating a correlation between epigenetic regulation and endometriosis, its precise genetic etiology remains elusive. Methyltransferase-like 14 (METTL14), a crucial component of the N6-methyladenosine (mA) RNA methyltransferase complex and an RNA binding scaffold, is known to play a pivotal role in various human diseases.

Trojan Horse-Like Biohybrid Nanozyme for Ameliorating Liver Ischemia-Reperfusion Injury.

Liver ischemia and reperfusion (I/R) injury is a reactive oxygen species (ROS)-related disease that occurs during liver transplantation and resection and hinders postoperative liver function recovery. Current approaches to alleviate liver I/R injury have limited effectiveness due to the short circulation time, poor solubility, and severe side effects of conventional antioxidants and anti-inflammatory drugs. Herein, a universal strategy is proposed to fabricate a Trojan horse-like biohybrid nanozyme (THBN) with hepatic-targeting capabilities.

Practice and application of learning curve theory in improving prescription review skills in standardized training for pharmacists in medical institutions.

To explore the practice and application of learning curve theory in improving prescription review skills in standardized training for pharmacists in medical institutions, and to provide reference for enhancing the effectiveness of standardized training for pharmacists in medical institutions. A retrospective analysis was conducted on the relevant data of 20 students who participated in our hospital's standardized pharmacist training in 2022 and 2023 during their prescription review practice learning process. The prescription review practice learning process is divided into 10 stages, with 100 prescriptions in each stage.

Mast cell-mediated microRNA functioning in immune regulation and disease pathophysiology.

Upon stimulation and activation, mast cells (MCs) release soluble mediators, including histamine, proteases, and cytokines. These mediators are often stored within cytoplasmic granules in MCs and may be released in a granulated form. The secretion of cytokines and chemokines occurs within hours following activation, with the potential to result in chronic inflammation.

Association between the immune-inflammation indicators and osteoarthritis - NHANES 1999-2018.

Background: Investigate the link between systemic immune-inflammatory index (SII) and Systemic Immune Response Index (SIRI) with osteoarthritis (OA) using National Health and Nutrition Examination Survey (NHANES) data (1999-2018).

Methods: Extracted NHANES data (1999-2018) and selected a study population based on demographic, examination, and laboratory data. Calculated SII (platelet count ​× ​neutrophil count/lymphocyte count) and SIRI (neutrophil count ​× ​monocyte count/lymphocyte count).

Impact of intermediate-dose tertiary prophylaxis on quality of life and psychological aspects of adult patients with severe/moderate hemophilia A.

Objectives: Whether intermediate-dose tertiary prophylaxis can improve quality of life and psychological health in adults with severe/moderate hemophilia A has not been determined. This research aims to explore the impact of intermediate-dose tertiary prophylaxis with recombinant human FVIII (rhFVIII) on quality of life, anxiety and depression in such individuals transitioned from on-demand treatment.

Methods: This retrospective analysis collected data from July 2019 to July 2022.

Efficacy of internet-delivered universal and tailored transdiagnostic interventions for anxiety and depression: A systematic review and meta-analysis of randomized controlled trials.

Background: Internet-delivered transdiagnostic psychological interventions are promising approaches for the treatment of anxiety and depression. This study aimed to review and synthesize evidence related to the effectiveness of Internet-based transdiagnostic interventions for reducing anxiety and depression, and to compare the effects of universal versus tailored interventions.

Methods: A Systematic review search was conducted on the PubMed, EMBASE, Cochrane Library, Web of Science, PsychINFO, Medline, and CINAHL databases as of 28 March 2024.

The relationship between psychological resilience, perceived social support, acceptance of illness and mindfulness in patients with hepatolenticular degeneration.

To investigates the current status of mindfulness in patients with Wilson's disease (WD) and to explore the effects of psychological resilience, perceived social support, and acceptance of illness on their mindfulness. By using the convenient sampling method, 136 patients with WD were selected from May 2019 to January 2023 in 12 tertiary hospitals in Chengdu. General information questionnaire, five facet mindfulness scale, resilience scale, acceptance of illness and perceived social support scale were used to conduct the investigation.

Comparative analysis of general and regional anesthesia applications in geriatric hip fracture surgery.

Background: This study compares the outcomes of general anesthesia (GA) and regional anesthesia (RA) in geriatric hip fracture surgery to determine optimal anesthesia strategies for this population.

Methods: A comprehensive literature review was conducted, analyzing studies comparing GA and RA in elderly patients undergoing hip fracture surgery. Studies encompassed various designs, including randomized controlled trials, cohort studies, and meta-analyses.

Synergistic effect of anemia and obstructive sleep apnea on hypertension: National Health and Nutrition Examination Survey 2015-2018.

Objective: Anemia, obstructive sleep apnea (OSA), and hypertension are common social health problems. They are interconnected. This study assessed the independent association of anemia and OSA with hypertension and the interaction between anemia and OSA on hypertension in the US population.

Effects of ultrasound-guided recruitment manoeuvres on postoperative pulmonary complications in laparoscopic bariatric surgery patients: study protocol for a randomised clinical trial.

Background: Lung ultrasound-guided alveolar recruitment manoeuvres (RMs) may reduce the lung ultrasound score. However, whether the use of this strategy can reduce the incidence of postoperative pulmonary complications (PPCs) in the adult obese population has not yet been tested.

Methods/design: This is a single-centre, two-arm, prospective, randomised controlled trial.

Ubiquitin-specific protease 25 ameliorates ulcerative colitis by regulating the degradation of phosphor-STAT3.

Ubiquitin-specific protease 25 (USP25), a member of the deubiquitination family, plays an important role in protein ubiquitination, degradation, inflammation, and immune regulation. However, the role and mechanism of USP25 in ulcerative colitis (UC) remain unclear. To study the role and mechanism of USP25 in UC, bioinformatics analysis and research are conducted on clinical patients with UC, Usp25 knockout (Usp25) mice, intestinal epithelial cell-specific knockout signal transducer and activator of transcription 3 (Stat3) (Villin-Cre Stat3) mice, and human colonic epithelial cells.

The association of serum total bile acids and deep venous thrombosis.

Background And Aim: Bile acid metabolism plays a crucial role in maintaining the delicate balance between coagulation and anticoagulation processes. However, there is a paucity of research exploring the relationship between serum total bile acids (TBA) levels and the risk of deep venous thrombosis (DVT) in Chinese individuals. The primary objective of this study is to investigate the association between TBA levels and DVT occurrence in this population.

Effects of transurethral 0bipolar plasma prostatectomy on urodynamics and sexual function in patients with prostatic hyperplasia.

This was an original article, and the objective of this study was to investigate the effects of bipolar transurethral plasma kinetic prostatectomy (TUPKP) on urodynamics and sexual function in benign prostatic hyperplasia (BPH) patients. One hundred and four BPH patients were divided into a control group and an intervention group. The control group received transurethral resection of prostate, while the intervention group received TUKEP.

The incidences of postoperative hypoparathyroidism during thyroid surgery with ipsilateral central lymph node dissection for papillary thyroid carcinoma were close to that during thyroid surgery for benign thyroid diseases necessitating surgical intervention: a retrospective study.

Objective: This study aimed to assess the degree of effect of central lymph node dissection on postoperative hypoparathyroidism incidence.

Methods: The incidence of postoperative hypoparathyroidism was compared between patients receiving thyroidectomy with central neck dissection for papillary thyroid carcinoma and those undergoing thyroidectomy for benign thyroid diseases (thyroid follicular adenoma and/or nodular goiter) necessitating surgical intervention.

Results: The incidence of postoperative hypoparathyroidism was not significantly different between the groups of lobe thyroidectomy for benign thyroid diseases and lobe thyroidectomy with ipsilateral central lymph node dissection for papillary thyroid carcinoma (immediate: 9.