Application and progress of high-throughput sequencing technologies in the research of hereditary hearing loss.

Hearing loss (HL) is the most common birth defect. Elucidating the genetic basis of hereditary deafness can not only assist diagnosis, provide the basis for genetic counseling and the prevention of deafness, but also bring a deeper understanding of the disease pathogenesis. In the genomic era, high-throughput sequencing technologies, represented by whole genome sequencing (WGS), whole exome sequencing (WES) or target region sequencing, have been widely used in the studies of hereditary HL.

Gradient between dorsalis pedis and radial arterial blood pressures during sevoflurane anaesthesia: A self-control study in patients undergoing neurosurgery.

Background: The dorsalis pedis artery (DPA) is a good alternative to the radial artery (RA) for invasive blood pressure monitoring when the upper limb is burned or injured, or if the RA is not available. Understanding the pattern of pressure difference between DPA and the commonly used RA during inhalational anaesthesia is helpful for haemodynamic management and therapeutic decisions.

Objectives: The objective of this study was to investigate the time-dependent variation of DPA-to-RA pressure gradient during sevoflurane anaesthesia and the overall difference between the two pressures during neurosurgery, together with the causes of the pressure gradient change.

Dorsalis pedis arterial pressure is lower than noninvasive arm blood pressure in normotensive patients under sevoflurane anesthesia.

Background: It is widely known that blood pressure (BP) in the lower extremity is higher than in the upper extremity. However, whether this phenomenon remains the same during general anesthesia is still unclear. This study aims to investigate the difference between invasive dorsalis pedis artery (DPA) pressure and the most commonly used noninvasive arm pressure during sevoflurane anesthesia.

Effects of pulsed electromagnetic field frequencies on the osteogenic differentiation of human mesenchymal stem cells.

The purpose of this study was to evaluate the effect of different frequencies of pulsed electromagnetic fields on the osteogenic differentiation of human mesenchymal stem cells. Third-generation human mesenchymal stem cells were irradiated with different frequencies of pulsed electromagnetic fields, including 5, 25, 50, 75, 100, and 150 Hz, with a field intensity of 1.1 mT, for 30 minutes per day for 21 days.

Regulatory effects of myoendothelial gap junction on vascular reactivity after hemorrhagic shock in rats.

Myoendothelial gap junction (MEGJ), one kind of gap junction between vascular endothelial cell and vascular smooth muscle cell, can transmit electrical and chemical signals to keep the electric and machinery activity synchronism of vasculature. After severe trauma or shock, vascular reactivity to vasoconstrictors or vasodilators is greatly reduced. However, whether MEGJ participates in the regulation of vascular reactivity after hemorrhagic shock, what type of MEGJ is involved, and what is the possible mechanism are unknown.

[TCM differentiation and treatment of fibromyalgia syndromes based on meridian science].

Fibromyalgia syndrome (FS) is a rheumatic and immunologic disease and the disease incidence is ranked at the third in the rheumatic diseases, characterized by whole-body myalgia, and with mental and nervous symptoms and signs. At present, there is no ideal therapy for it. Based on TCM theories, this article tries to analyze the pathogenesis of FS from the angle of meridian and collateral science, and according to location of pain of the patient, TCM syndrome differentiation for corresponding channels and collaterals is made and FS possible relation with dysfunction of qi is raised, which is used to guide the treatment, so as to provide a better physical treatment way.

[Influence of CD4+ and CD25+ T regulatory cell on mouse bearing gastric tumor in vivo].

Objective: To investigate the influence of CD4+ CD25+ regulatory T cells(Treg cells) on mouse gastric cancer.

Methods: Treg cell in mouse spleen bearing gastric tumor was tested in different time points. Magic cell sorting(MACS) method was used to purify mouse Treg cells and the Treg cells were injected into mouse bearing gastric tumor with different dosage.

[Regulatory effect of protein kinase C and protein kinase G on calcium sensitivity of vascular smooth muscle cells following hemorrhagic shock].

Objective: To observe the effect of protein kinase C (PKC) and protein kinase G (PKG) on calcium desensitization following hemorrhagic shock in rats.

Methods: The model of hemorrhagic shock was replicated by blood letting and maintaining mean arterial pressure at 40 mm Hg (1 mm Hg=0.133 kPa) for 2 hours.

[Expression of CD44v3 and its prognostic value in non-small cell lung cancer].

Background: To study the expression of CD44v3 and its prognostic value in non-small cell lung cancer (NSCLC).

Methods: CD44v3 expression was detected in 52 NSCLC tissues and 12 normal pulmonary tissues by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry (SP method).

Results: The positive expression rate of CD44v3 in NSCLC was 57.