1 results match your criteria: "the Second Affiliated Hospital of General Hospital of Chinese PLA[Affiliation]"
[Construction of GJB2 mutations common in Chinese EGFP fusion protein vectors].
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
August 2009
Department of Otolaryngology, the Second Affiliated Hospital of General Hospital of Chinese PLA, Beijing, 100091, China.
Objective: To construct GJB2 gene mutations common in Chinese EGFP fusion protein vectors, and to search for better way to study the mechanism of deletion mutations in GJB2 gene.
Method: Non-fusion protein vectors of 235delC, 299-300 del AT and 176 del 16 bp were first made by point mutation methods in vitro. Then expression part of the upper 3 mutations were amplified by PCR and the PCR products were cloned into TA cloning vector.