Potential synergistic effects of a mixture of mineral trioxide aggregate (MTA) cement and Bacillus subtilis in dental caries treatment.

Bacillus subtilis is nonpathogenic in humans and produces a number of useful substances and, therefore, this bacterium is used in probiotic therapy. There have been trials of B. subtilis for patients with periodontitis, but not for patients with caries.

Formation of vacuum-formed and pressure-formed mouthguards.

Background/aim: The method used to form mouthguards should be carefully selected in order to obtain their full preventive benefits. The aim of this study was to examine the differences of mouthguard characteristics according to the forming methods.

Materials And Methods: Mouthguard sheets of 3.

Influence of continuous use of a vacuum-forming machine for mouthguard thickness after thermoforming.

Background/aims: Mouthguards can reduce the risk of sports-related injuries such as tooth fracture or avulsion, but the sheet material and thickness have a large effect on their efficacy and safety. The aim of this study was to investigate the effect of the continuous use of a vacuum-forming machine on mouthguard thickness.

Materials And Methods: Ethylene vinyl acetate sheets and olefin copolymer sheets were used for thermoforming with a vacuum-forming machine.

Periosteal distraction osteogenesis versus immediate periosteal elevation in a rat model: Histological and micro-CT analysis.

Purpose: The aim of the present study was to compare periosteal distraction osteogenesis (PDO) to immediate periosteal elevation (IPE) in terms of de novo bone formation.

Material And Methods: Animals of PDO Group were subjected to a 7-day latency period and a 10-day distraction period. Distraction device in IPE Group were activated for 1 mm at placement.

Histological evaluation of direct pulp capping with all-in-one adhesives in rat teeth.

The aim of this study was to histologically evaluate direct pulp capping using different all-in-one adhesives in rat teeth. Five all-in-one adhesives and a control material (MTA) were used. Each material was applied on the exposed pulp, and each cavity was subsequently restored with the resin composite.

Shear bond strengths of tooth coating materials including the experimental materials contained various amounts of multi-ion releasing fillers and their effects for preventing dentin demineralization.

We examined shear bond strengths (SBSs) of various tooth-coating-materials including the experimental materials to dentin and demineralization resistance of a fractured adhesive surface after the SBS testing. Three resin-type tooth-coating-materials (BC, PRG Barrier Coat; HC, Hybrid Coat II; and SF, Shield force plus) and two glass-ionomer-type tooth-coating-materials (CV, Clinpro XT Varnish; and FJ, Fuji VII) were selected. The experimental PRG Barrier Coat containing 0, 17, and 33 wt% S-PRG filler (BC0, BC17, and BC33, respectively) were developed.

Collagen gel droplet-embedded culture drug sensitivity testing in squamous cell carcinoma cell lines derived from human oral cancers: Optimal contact concentrations of cisplatin and fluorouracil.

The collagen gel droplet-embedded culture drug sensitivity test (CD-DST) is an anticancer drug sensitivity test that uses a method of three-dimensional culture of extremely small samples, and it is suited to primary cultures of human cancer cells. It is a useful method for oral squamous cell carcinoma (OSCC), in which the cancer tissues available for testing are limited. However, since the optimal contact concentrations of anticancer drugs have yet to be established in OSCC, CD-DST for detecting drug sensitivities of OSCC is currently performed by applying the optimal contact concentrations for stomach cancer.

Influence of working model angle on the formation of a pressure-formed mouthguard.

Background/aim: Custom-made mouthguards are fabricated on working models. However, the influence of the working model to the mouthguard characteristics has not been clearly established. The aim of this study was to examine the influence of the angle of the working model on the formation of a pressure-formed mouthguard.

Shape change in mouthguard sheets during thermoforming - part 2: effect of the anteroposterior position of the model on mouthguard thickness.

Background/aim: Mouthguards can reduce the risk of sports-related injuries, but the sheet material and thickness have a large effect on their efficacy and safety. The aim of this study was to investigate the effect of model position in the molding machine on the reduction in mouthguard thickness.

Materials And Methods: Ethylene vinyl acetate sheets and olefin copolymer sheets were used for thermoforming by a pressure- or a vacuum-forming machine.

Effects of collagen membrane application and cortical bone perforation on de novo bone formation in periosteal distraction: an experimental study in a rabbit calvaria.

Objectives: The aim of the present study was to assess the impact of collagen membrane application and cortical bone perforations in periosteal distraction osteogenesis.

Study Design: A total of 32 New Zealand rabbits were randomized into four experimental groups, considering two treatment modalities. Calvarial bone was perforated or left intact (P+/-).

In vitro immunological and biological evaluations of the angiogenic potential of platelet-rich fibrin preparations: a standardized comparison with PRP preparations.

Background: Platelet-rich fibrin (PRF), a platelet-rich plasma (PRP) derivative mainly composed of fibrin networks, has been increasingly demonstrated to be effective in wound healing in clinical and pre-clinical animal studies. However, there has still been a concern that major growth factors may significantly be loss from PRF during its preparation through the slow clotting process. To address this concern, we compared the angiogenic potential of PRF and PRP by standardization of procedures based on volume ratios.

Non-invasive, quantitative assessment of the morphology of γ-irradiated human mesenchymal stem cells and periosteal cells using digital holographic microscopy.

Purpose: To assure the quality of cells to be used in cell therapy, we examined the applicability of digital holographic microscopy (DHM) for non-invasive, quantitative assessment of changes in cell morphology.

Materials And Methods: Mesenchymal stem cells derived from adipose tissue (MSC-AT) and bone marrow (MSC-BM), in addition to human alveolar periosteal cells (PC) as a reference, were γ-ray irradiated (1 and 4 Gy), and their morphological changes were quantified without fixation using holographic microscopy. After detachment and fixation with ethanol, cell number and surface antigen expression were determined using an automated cell counter kit and flow-cytometry, respectively.

Appropriate fabrication method for vacuum-formed mouthguards.

Aim: The aim was to examine the influence of the heating temperature on the fabrication of vacuum-formed mouthguards.

Materials And Methods: Mouthguard sheets of 3.8 mm ethylene vinyl acetate were vacuum-formed on working models at three heating temperatures: 80, 100, and 120°C.

Dual-Labeled Near-Infrared/(99m)Tc Imaging Probes Using PAMAM-Coated Silica Nanoparticles for the Imaging of HER2-Expressing Cancer Cells.

We sought to develop dual-modality imaging probes using functionalized silica nanoparticles to target human epidermal growth factor receptor 2 (HER2)-overexpressing breast cancer cells and achieve efficient target imaging of HER2-expressing tumors. Polyamidoamine-based functionalized silica nanoparticles (PCSNs) for multimodal imaging were synthesized with near-infrared (NIR) fluorescence (indocyanine green (ICG)) and technetium-99m ((99m)Tc) radioactivity. Anti-HER2 antibodies were bound to the labeled PCSNs.

Effect of thermal shrinkage during thermoforming on the thickness of fabricated mouthguards: Part 2 pressure formation.

Background: The aim of this study was to examine the effect of thermal shrinkage, which occurs during thermoforming of ethylene vinyl acetate (EVA) sheets on the thickness of mouthguards fabricated by pressure formation.

Materials And Methods: Mouthguards were fabricated from 4.0-mm-thick EVA sheets by utilizing a pressure-forming machine.

Characterization of a PTH1R missense mutation responsible for Jansen type metaphyseal chondrodysplasia.

Parathyroid hormone and parathyroid hormone-related peptide (PTHrP), and its receptor (PTH1R) play an important role in differentiation of bone and cartilage in the developing stages. Constitutive dimers of PTH1R are believed to be dissociated by ligand binding, and monomeric PTH1R is capable of activating G protein. Jansen type metaphyseal chondrodysplasia is caused by missense mutations in PTH1R, which are constitutively active even without the presence of the ligands.

Evaluation of reliability of perioral muscle pressure measurements using a newly developed device with a lip piece.

Purpose: We examined the reliability of measurements using a newly developed perioral muscle pressure measuring device with a lip piece in healthy adults.

Methods: Subjects were 40 healthy men (25.8 years) with normal stomatognathic function.

Single-staged vs. two-staged implant placement in vertically deficient alveolar ridges using bone ring technique - Part 2: implant osseointegration.

Objectives: The aim of this study was to evaluate the osseointegration of implants placed in a single-staged compared to two-staged procedure using bone ring technique.

Material And Methods: In this study were used standardized, vertical alveolar bone defects in dogs. In the test group, dental implants (Straumann BL , Basel, Switzerland) were inserted simultaneously with bone ring technique.

Effect of high-glucose conditions on human periodontal ligament endothelial cells: in vitro analysis.

Endothelial cells participate in key aspects of vascular biology, such as maintenance of capillary permeability and regulation of inflammation. According to previous reports, endothelial cells have revealed highly specific characteristics depending on the organs and tissues. In particular, periodontal endothelial cells have a higher permeability than vascular endothelial cells of other types of tissue.

Influence of working model position on the formation of a pressure-formed mouthguard.

Aim: The aim of this study was to examine the influence of the position of the working model on the formation of a pressure-formed mouthguard.

Materials And Methods: Mouthguard sheets of 3.8 mm ethylene vinyl acetate were pressure-formed on working models in three positions: anterior, central, and posterior.

Optimal heating condition of mouthguard sheet in vacuum-pressure formation: part 3 styrene-based thermoplastic elastomer.

Background: The aim of this study was to identify suitable heating conditions of polyolefin-polystyrene co-polymer sheets in vacuum-pressure formation, monitor the sheet temperature during molding, and examine the thickness of the fabricated mouthguard.

Materials And Methods: Mouthguards were fabricated with polyolefin-polystyrene co-polymer sheets (4.0-mm thick) utilizing a vacuum/pressure-forming device, which was subjected to vacuum forming for 10 s and pressure molding for 2 min.

The relationship between the incisor position and lingual surface morphology in normal occlusion.

This study aimed to investigate the relationship between the morphological characteristics of maxillary incisors and the anterior occlusion. The study materials comprised dental casts and lateral cephalograms of 26 modern Mongolian females with Angle Class I normal occlusion (mean age, 21 years 5 months). Computed tomography (CT) images of the dental casts were taken with an X-ray micro-CT system (SMX-100CT, Shimadzu, Kyoto Japan).