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Retrospective quantitative detection of SARS-CoV-2 by digital PCR showing high accuracy for low viral load specimens.
J Infect Dev Ctries
January 2022
Infectious Diseases Division, the Nantong Third Hospital Affiliated to Nantong University, Nantong, P. R. China.
Introduction: Accurate detection of severe acute respiratory syndrome coronavirus 2 is critical for diagnosis and disease status evaluation of Coronavirus disease 2019. We retrospectively evaluated the infection status and viral load of severe acute respiratory syndrome coronavirus 2 in Nantong city, China, using a quantitative digital polymerase chain reaction and reverse-transcription PCR.
Methodology: A total of 103 clinical specimens from 31 patients were collected and tested by digital PCR and reverse-transcription PCR.