10 results match your criteria: "the Medical Innovation Research Division of Chinese PLA General Hospital[Affiliation]"
STAR Protoc
December 2024
RocRock Biotechnology (Suzhou), Suzhou 215000, China. Electronic address:
Human-derived macrophages are notoriously difficult to infect with HIV-1-based lentiviruses, posing a limitation to the advancement of chimeric antigen receptor macrophage (CAR-M) therapy. Here, we present a protocol for generating human chimeric antigen receptor (CAR)-engineered macrophages using the viral protein Vpx (encoded by the Sooty Mangabey simian immunodeficiency virus [SIV] and HIV-2 lineages) incorporated into the lentivirus vector, which enhances infection efficiency. We describe steps for cell cultivation, lentivirus production, concentration, infection procedures, and efficiency assessments.
View Article and Find Full Text PDFCell Immunol
September 2024
Research Center of Bioengineering, the Medical Innovation Research Division of Chinese PLA General Hospital, Beijing 100853, China. Electronic address:
The high plasticity and long-term persistency make macrophages excellent vehicles for delivering anti-tumor cytokines. Macrophage delivery of chemokines and cytokines shows potential in tumor therapy. TRAIL, a promising anti-tumor cytokine, induces apoptosis in tumor cells with low toxicity to normal cells.
View Article and Find Full Text PDFBiochem Biophys Rep
September 2024
Applied Biology Laboratory, College of Environmental and Safety Engineering, Shenyang University of Chemical Technology, Shenyang, 110142, China.
Chimeric antigen receptor (CAR)-modified macrophages are a promising treatment for solid tumor. So far the potential effects of CAR-M cell therapy have rarely been investigated in hepatocellular carcinoma (HCC). Glypican-3 (GPC3) is a biomarker for a variety of malignancies, including liver cancer, which is not expressed in most adult tissues.
View Article and Find Full Text PDFHeliyon
December 2023
Applied Biology Laboratory, College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang, 110142, China.
Genetically modified macrophage infusion has been proven to be a novel treatment for cancer. One of the most important processes in macrophage-based therapy is the efficient transfer of genes. HIV-1-derived lentiviruses were widely used as delivery vectors in chimeric antigen receptor T and NK cell construction.
View Article and Find Full Text PDFIdiopathic pulmonary fibrosis (IPF) is a fatal interstitial lung disease characterized by chronic, progressive, and fibrotic lung injury. Although remarkable progress has been made toward understanding the pathogenesis of PF, finding more effective treatments for this fatal disease remains a challenge. In this study, we describe an innovative macrophage-based approach to deliver anti-fibrotic protein to the lung and inhibit PF in a mouse model of bleomycin (BLM)-induced lung injury.
View Article and Find Full Text PDFFront Surg
November 2022
Research Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Division of Chinese PLA General Hospital, Beijing, China.
Background: Communication between fibroblasts and endothelial cells is essential for skin wound repair and regeneration. Extracellular vesicles (EVs) are crucial for intracellular communication by transporting active molecules. However, whether EVs derived from diabetic fibroblasts can perform the nomal communication function is unclear.
View Article and Find Full Text PDFCell Prolif
March 2023
Department of Obstetrics and Gynecology, The First Medical Center of Chinese PLA General Hospital, Beijing, China.
Aging (Albany NY)
July 2022
Department of Clinical Biobank Center, The Medical Innovation Research Division of PLA General Hospital, Beijing, 100853, China.
Aging (Albany NY)
May 2022
Department of Clinical Biobank Center, The Medical Innovation Research Division of PLA General Hospital, Beijing 100853, China.
Aging causes a decline in ovarian function and may contribute to ovarian failure and infertility. We investigated the effect of menstrual blood-derived mesenchymal stem cells (MenSCs) and their mitochondria on ovarian function in aged mice. We performed two treatment protocols: i) ovaries of recipient aged mice were treated with MenSCs 3D alginate gel; ii) ovaries were injected with mitochondria suspension and then incubated with mitochondrial 3D gel.
View Article and Find Full Text PDFCell Death Dis
July 2021
Department of Biomedical Engineering, the Fifth medical Centre, Chinese PLA General Hospital, Beijing, 100071, China.
Receptor interaction protein kinase 1 (RIPK1) plays a diverse role in tumor necrosis factor α (TNFα) signalings. The ubiquitination of RIPK1 is essential for NF-κB activation, whereas its kinase activity promotes apoptosis and necroptosis. However, the mechanisms underlying have not been fully illuminated.
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