The study aimed to enhance the selectivity of mannitol-yolk-polymyxin B agar (MYPA) for detecting Bacillus cereus by adding cefuroxime, a second-generation cephalosporin.
Cefu-MYPA showed better performance than original MYPA in recovering B. cereus from 60 food samples, with average counts of 462.0 CFU/g compared to 363.5 CFU/g for MYPA.
The modified medium significantly reduced the presence of competing flora, showing improved detection rates of B. cereus in most food items tested, indicating its effectiveness, especially in foods that typically have many other microorganisms present.