[Studies on the blood group serologic characters of enzyme-only red cell antibody].

The objective of this study was to investigate the serologic characters of enzyme-only red cell antibody and its clinical significance, and to provide basis for the safety of blood transfusion. The patient serum containing enzyme-only antibody was used to react with the red cells of donors, panel cells and auto-cells in various medium. Absorption and elution test were also per formed.

[Detection of the anti-SARS-coronavirus specific antibody levels in 156 SARS patients].

The objective of this study was to explore the development of IgG and IgM against SARS CoV and characteristics of changes of antibody titers in patients with severe acute respiratory syndrome (SARS) and to search the opportunity for collecting specific anti-serum from convalescent patients with SARS. The anti-SARS-coronavirus specific antibody levels in 156 SARS patients were measured with ELISA. The results showed that the total positive rates of IgG and IgM were 75.

[The approaches of chronic graft-versus-host disease].

Chronic graft-versus-host disease (cGVHD) continues to be a major complication in long-term survivors after allogeneic hematopoietic stem cell transplantation and is the principal cause of morbidity and non-relapse mortality. With the new approaches in the immune mechanisms of cGVHD, the diagnosis, prophylaxis and treatment of cGVHD are involved. This review summarises the current progress of research on cGVHD.

[Determination of tolerance ability of platelet to the change of solution osmotic pressure and its significance].

In order to determine the tolerance ability of platelet to change of osmotic pressure in solution, the isotonic fresh platelets were exposed to a series of crystal salt solutions with osmotic pressure range from 47 to 611 mOsm for 15 minutes. Then the platelets were returned to isotonic condition and kept for 15 minutes. The expressions of phosphatidylserine and CD62p were assayed in platelets.

[Non-Myeloablative Allogeneic Hematopoietic Stem Cell Transplantation-New Opinion about Hematopoietic Stem Cell Transplantation].

Non-myeloablative allogeneic hematopoietic stem cell transplantation (NMAT) is brought forward recent years. It is a modified transplant technology. NMAT treats malignant disease by graft versus tumor effect of chimerism after transplantation.

[Activation and Cytotoxicity of Bone Marrow Immunocytes by Using Various Cytokines].

The study aimed to explore the changes of the immunocyte quantities and cytotoxicity in bone marrow, and how many hematopoietic progenitor cells retained after the bone marrow cells were activated by different combinations of various cytokines. Bone marrow cells were divided into four groups and were cultured in vitro: (1) Control: no cytokines were added. (2) IL-2 group: bone marrow cells were activated by IL-2.

[Morphology and Immunophenotype Study of Bone Marrow Mononuclear Cells after Activated by Different Cytokines In Vitro].

Bone marrow cells in cultures were divided into four groups and cultured with various cytokines in vitro. These four groups are: control, IL-2 group, CD3-AK group, and CIK group. The morphological (cell volume, nucleus/plasm) changes of bone marrow cells in culture were observed.

[A Simplified Method for Cryopreservation of Peripheral Blood Stem Cells].

To simplify the traditional method for cryopreservation of peripheral blood stem cells(PBSCs) at -196 degrees C with rate-controlled freezing with 10% dimethyl sulfoxide(DMSO), the simplified method was carried out by freezing the cells to -80 degrees C in low-temperature freezer with the combination of 5% DMSO, 3% hydroxyethyl starch(HES) and 4% human serum albumin(HSA) as cryoprotectant. PBSCs were cryopreserved by different methods. Cell viability and recovery rate of mononuclear cells (MNC), CFU-GM and CD34(+) cells were compared.

[Flow cytometry combined assay for phosphatidylserine and CD62p expressed by preserved platelets].

Human platelets have distinct characters when preserved by different methods. A efficient flow cytometric assay for different preserved platelets expression of CD62p and phosphatidylserine(PS) is in dire need. Efficient flow cytometric assay for CD62p and PS expressed by preserved platelets was established and the major conditions were optimized.

[The expression of Fas, FasL and Bcl-2 on RMA cells during the process of apoptosis induced by chemotherapeutic drugs].

The objective of the study is to explore the effect of Fas, FasL and Bcl-2 on the process of apoptosis induced by chemotherapeutic drugs through detecting the expression of Fas, FasL and Bcl-2 on murine lymphoma cell line RMA. Dexamethasone(DEX), etoposide (VP-16), arsenic trioxide As(2)O(3) and all trans-retinoic-acid (ATRA) were added to the RMA cells as well as to the cells preincubated with interleukin-2 (IL-2), interleukin-6 (IL-6) or granulocyte-macrophage colony-stimulating factor (GM-CSF), respectively. The effect on apoptosis was observed and the expression of Fas and FasL mRNA as well as the expression of Fas and Bcl-2 antigen were measured.

[Comparison of leucocyte-reduced platelet concentrates produced with spectra version 5.1 and version 7.0 blood cell separators].

In the present study, the performance of a new blood cell separator (COBE Spectra LRS Turbo Version 7.0) and that of the previous version LRS version 5.1 in the collection efficiency (CE), collection rate and residual white blood cells during platelet collection from donors were compared.

[CD62p expression in platelet during the preparation course of Cryopreservated platelet-rich plasma].

In order to explore the factors that affect CD62p expression in platelet during the whole course of cryopreservated platelets preparation, CD62p expression of platelet was evaluated by flow cytometry assay. The whole course of cryopreservated platelets preparation in order included whole blood collection, centrifugation for fresh platelet-rich plasma preparation, addition of dimethyl sulfoxide, and freeze in -80 degrees C refrigerator and thaw in 38 degrees C water bath. Result showed that the CD62p expressed slowly from whole blood collection to addition of dimethyl sulfoxide, but expressed abruptly after freeze and thaw and it occupied 82 per cent of the whole expression.

[Change of adhesion molecule expression on CD34(+) cells from bone marrow and peripheral blood during mobilization with combination of chemotherapy and G-CSF].

To explore the dynamic change of CD34(+) cell expressing adhesion molecules in bone marrow and peripheral blood during mobilization with combination of chemotherapy and G-CSF and its clinical significance, mononuclear cells of bone marrow and peripheral blood from malignant hematopathy cases before and after mobilization with G-CSF were labeled by CD45-CY-Chrome, PE conjugated anti-CD34, and FITC conjugated anti-CD44, anti-CD49d, anti-CD62L and anti-CXCR4. For three-color fluorescence analysis by flow cytometry was performed on a FACScalibur. Also the relationship between the number of subpopulations in different expressions of adhesion molecules infused and the time of recovery in different blood cells after transplantation was evaluated.

[Comparative study on activated immunocytes of human bone marrow and peripheral blood by cytokines].

To study immunophenotype and cytotoxicity of the immunocytes in bone marrow and peripheral blood after activation by combined cytokines, mononuclear cells (MNC) of bone marrow and peripheral blood were activated by IFN-gamma, IL-1, IL-2 and McAb-CD3 in vitro. The cell amount and morphology during culture were observed. Cytochemical staining and immunophenotype analysis were done before and after culture in two groups of MNC.

[Thermometry of intracellular ice crystal formation in cryopreserved platelets].

The temperature of platelet intracellular ice crystal formation (IIF) is one of the most important physical parameters to instruct platelet cryopreservation. In this study, the range of temperatures for platelet IIF was measured by means of biological and physical methods. All platelet samples were graded cooling, and two samples of per 5 degrees C decrease were thawed by 2 different ways: 37 degrees C directly (T 37 degrees C) and 37 degrees C after keeping in liquid nitrogen (LN) for 2 hours.

[The effect of separating red blood cells from bone marrow graft in vitro by methylcellulose].

It has need to separate red blood cells (RBC) from marrow graft in ABO group unmatched BMT and auto-BMT with purging tumor cells, the separating effect of methylcellulose was observed. The mixture of 0.5% methylcellulose and bone marrow was laid up in an open transfusion system, and then sedimentation of RBC was performed in the transfusion tube.

Efficacy of domestic bisoprolol,enalapril and nifedipine retard in mild to moderate hypertension: a randomized double-blind multicenter clinical trial in China.

Objective: To compare the efficacy and tolerability of bisoprolol, enalapril and nifedipine retard in the treatment of mild to moderate essential hypertension.

Methods: This is a randomized, double-blind, multicenter clinical trial. 331 patients with mild to moderate hypertension in the army troop were randomly allocated to receive domestic bisoprolol ( n =110,5 mg/d),enalapril ( n =111,10 mg/d) and nifedipine retard ( n =110,20 mg/day).

[Implantation of stage II anterior chamber intraocular lens in aphakic eye after vitrectomy].

Objective: To sum up the clinical methods and results of stage II anterior chamber intraocular lens (AC-IOL) implantation in 54 aphakic eyes after vitrectomy.

Methods: Traditional corneoscleral limbal and tunnel incision were respectively used to perform stage II AC-IOL implantation separately in 54 aphakic eyes following vitrectomy.

Result: All 54 eyes had obtained good visual acuity and reached the corrected visual acuity before operation.

[Inhibition of ERK1/2 activity and c-fos mRNA after coronary artery balloon injury by intracoronary radiation in swine].

The effect of intracoronary radiation on extracellular signal regulated kinase 1/2 (ERK1/2) activity and c-fos mRNA after coronary artery balloon injury was investigated in swine. Twenty three swines were randomly divided into a radiation group and a control group after coronary balloon over stretch. The dilated segments in the radiation group were exposed to a dose of 20 Gy by a catheter based radiation system.

[The relationship between expression of lung resistance-related protein gene or multidrug resistance-associated protein gene and prognosis in newly diagnosed acute leukemia].

Objective: To evaluate the relationship between the expression of lung resistance-related protein (lrp) gene or multidrug resistance-associated protein (mrp) gene and prognosis in untreated acute leukemia (AL) patients.

Methods: Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to examine the expression of lrp and mrp gene in 58 newly diagnosed AL patients.

Results: The positive rate of lrp and mrp gene expression in newly diagnosed acute lymphocytic leukemia (ALL) group was 15.

[Analysis of the changes of temporomandibular joint under repeated +Gz stress].

Changes of temporomandibular joint (TMJ) under repeated +Gz stress were discussed. From the etiological point of view in TMJ, many papers in the fields of aviation medicine, microcirculation, maxillofacial surgery and bone surgery were reviewed. +Gz forces can cause inadequacy of blood of oxygen supply to TMJ area.

[Mechanical ventilation therapy for aged patients with severe acute left heart failure combined with respiratory failure].

Objective: To sum up the clinical experience of mechanical ventilation in treatment of aged patients with severe left heart failure (ALVF) combined with acute respiratory failure (ARF).

Methods: A retrospective analysis was carried out of the pattern, improvement rate, mortality, and complication of medical ventilation applied on 102 aged patients (82.0 9.

[The clinical significance of lung resistance-related protein gene (lrp), multidrug resistance-associated protein gene (mrp) and mdr-1/p170 expression in acute leukemia].

Objective: To evaluate the relationship between lrp, mrp, mdr-1/p170 and multidrug resistance in acute leukemia (AL).

Methods: 85 AL patients were divided into three groups: untreated (A), complete remission (B) and relapsed/refractory (C). The expression of lrp, mrp and mdr-1 mRNA was detected with RT-PCR assay and that of p170 measured with immunocytochemistry.

[The changes of density of platelet glycoprotein (GP) II b/III a receptor in the perioperative period of gastrectomy in aged rats].

Aim: To investigate changes of density of platelet glycoprotein (GP) II b/III a receptor in the perioperative period of gastrectomy in aged rats.

Methods: The subtotal gastrectomy operation was performed in 16 adult rats and 16 aged rats respectively. Platelet surface density of GP II b/III receptors were determined by flow cytometric analysis before and 6, 12, 24, 48, 72 hours after operation.

[A new mutation in the connexin 32 gene was found in Charcot- Marie-Tooth disease in Chinese patients].

Objective: To investigate the characteristics of gene mutations of connexin 32 exon 2 in Charcot-Marie-Tooth disease in Chinese patients.

Methods: Screening for connexin 32 gene mutation was conducted in 6 unrelated CMT1 patients without duplication and 10 unrelated CMT2 patients. Mobility shift of exon 2 was analyzed by SSCP and further confirmed by sequencing.