[Application of vacuum sealing drainage to treat late-stage large skin avulsion injury with infection].

Objective: To investigate the clinical effect of vacuum sealing drainage (VSD) on late-stage large skin avulsion injury with infection.

Methods: From May 2007 to August 2008, 9 patients with large-area skin avulsion injury and infection were treated. There were 1 male and 8 females aged 9-52 years old (median 27 years old).

Comparison of ablation zone between 915- and 2,450-MHz cooled-shaft microwave antenna: results in in vivo porcine livers.

Objective: The objective of our study was to compare the ablation zones of two cooled-shaft microwave antennae-one operating at 915 MHz and one operating at 2,450 MHz-in in vivo porcine livers.

Conclusion: The 915-MHz cooled-shaft microwave antenna can lead to deeper penetration of liver tissue and can yield a significantly larger ablation zone than the 2,450-MHz cooled-shaft antenna in in vivo porcine livers. In light of the result, 915-MHz cooled-shaft microwaves may be more effective for tumor ablation, especially for large tumors.

[Methylation of Id4 gene promoter in 4 malignant hematopoietic cell lines].

This study was purposed to investigate the status of Id4 gene promoter methylation in malignant hematologic cell lines. MS-PCR methods were used to detect the status of Id4 gene methylation in K562, HL-60, Ramous and CA46. Meanwhile bone marrow cells in healthy individuals and Hek937, a benign renal cell line, were involved as control.

[The significance of p16 protein and Ki-67 antigen expression in gastric mucosa-associated lymphoid tissue lymphoma].

Objective: To detect the expression of p16 protein and Ki-67 antigen in gastric mucosa-associated lymphoid tissue lymphoma (MALTL) and normal gastric mucosa and to investigate the clinical significance of their expression in the occurrence and development of gastric MALTL.

Methods: 47 samples of gastric MALTL diagnosed pathologically in the department of pathology of the PLA General Hospital from March 1993 to June 2005 were collected. By using immunohistochemical methods, the expression of p16 protein and Ki-67 antigen was detected in 20 samples of normal gastric tissue and the 47 samples of gastric MALTL.

Overexpression of TIMP-2 mediated by recombinant adenovirus in rat abdominal aorta inhibits extracellular matrix degradation.

To investigate the effects of a tissue inhibitor of the matrix metalloproteinase-2 (TIMP-2) gene on the extracellular matrix of the abdominal aorta, models of rat abdominal aortic aneurysm were utilized and a solution of AdTIMP-2 was perfused into the abdominal aorta. The models of rat abdominal aortic aneurysm were constructed with intraluminal perfusion of the abdominal aorta with porcine pancreatic elastase, and an adenovirus solution carrying the TIMP-2 gene was transferred into the aorta by local perfusion. After 14 days, aortic wall elastin and collagen concentrations were measured with an image analysis system and fixed aortic tissues were examined by light microscopy for inflammation.

[The methylation pattern and clinical significance of Zonula occludens-1 gene promoter in acute leukemia].

Objective: To investigate the methylation status of Zonula occludens-1 (ZO-1) gene promoter and discuss its role in the pathogenesis and progression of acute leukemia (AL) as a general gene marker.

Methods: The methylation pattern in promoter region of ZO-1 gene was detected with methylation specific PCR in AL cell lines HL60, Molt4 and NK92 as well as in 121 clinical bone marrow samples including 81 cases of AL and 40 non malignant cases.

Results: The promoter region of ZO-1 gene was completely methylated in HL60, Molt4 and NK92 cells; but it was unmethylated in 40 non malignant bone marrow samples.

Staged surgical treatment for severe and rigid scoliosis.

Background: A retrospective study of staged surgery for severe rigid scoliosis. The purpose of this study was to evaluate the result of staged surgery in treatment of severe rigid scoliosis and to discuss the indications.

Methods: From 1998 to 2006, 21 cases of severe rigid scoliosis with coronal Cobb angle more than 80 degrees were treated by staged surgeries including anterior release and halo-pelvic traction as first stage surgery and posterior instrumentation and spinal fusion as second stage.

[Change of IL-4 and IL-6 levels in blood plasma of patients transplanted with allogeneic hematopoietic stem cells determined by protein chip and its relationship with aGVHD].

This study was aimed to explore the relationship between changes of IL-4, IL-6 levels in blood plasma of patients receiving allo-HSCT and aGVHD through dynamical detection. The IL-4 and IL-6 levels in peripheral blood were detected by protein chip and were observed for 10 weeks. The results indicated that the IL-4 level increased rapidly in 1 week after transplantation in aGVHD group and was higher than that of non aGVHD group (p<0.

[Allogeneic peripheral blood hematopoietic stem cell transplantation for post-operatively treating acute non-lymphocytic leukemia patient complicated with renal cell carcinoma: one case report].

The aim of this study was to evaluate the safety and efficacy of allogeneic hematopoietic peripheral blood stem cell transplantation (allo-PBHSCT) for post-operative therapy of acute non-lymphocytic leukemia (ANLL) patient complicated with renal cell carcinoma (RCC). One ANLL patient complicated with RCC underwent an myeloablative HLA-identical relative allo-PBHSCT after RCC operation. The conditioning regimen consisted of total body irradiation, cyclophosphamide and cytarabine.

[Regulation of immunity by sphingosine 1-phosphate and its G protein-coupled receptors--review].

Sphingosine 1-phosphate (S1P) is an important biologically active lysophospholipid that transmits signals through a family of G-protein-coupled receptors (GPCRs) to regulate the vital functions of several types of immune cells. The S1P GPCRs suppress both generation of specialized functional cytokines, such as IFN-gamma and IL-4, and proliferation of T-cells. Although S1P is chemotactic to T cells, B cells, dendritic cells, and natural killer cells, the major effect of S1P on the immune system is the regulation of lymphocyte recirculation and tissue distribution by S1P and S1P1.

[Methylation of Id4 gene promoter in acute leukemia].

This study was purpose to investigate the difference of Id4 gene promoter methylation between healthy individuals and acute leukemia patients. MS-PCR methods were used to detect the status of Id4 gene methylation in healthy individuals and acute leukemia patients. The results showed that Id4 gene was unmethylated in bone marrow samples from healthy individuals.

Nerve regeneration and functional recovery after a sciatic nerve gap is repaired by an acellular nerve allograft made through chemical extraction in canines.

The purpose of the present study is to test whether chemically extracted acellular nerve segments can be used to repair the sciatic nerve gap. Fifteen canines were divided into acellular nerve allografting group (ANG, six canines), autografting group (AG, six canines), and fresh nerve allografting group (FNG, three canines). The sciatic nerves on the right side of the animals were exposed, and 5-cm-long segments of the nerves were removed from the midthigh level and replaced by the three types of grafts.

[Construction of recombinant adenovirus vector of mouse keratinocyte growth factor].

Objective: To construct a recombinant adenovirus vector expressing keratinocyte growth factor (KGF) gene in mouse.

Methods: KGF gene amplified from mouse cDNA by polymerase chain reaction (PCR). It was reverse transcript from RNA that had been harvested from C57BL/6 mouse, and then inserted into the plasmid pShuttle-CMV to construct the shuttle plasmid pShuttle-CMV-KGF (pKGF).

[Repair of immedicable ulcer in skull cap with expanded bipedical axial flap in senile patients].

Objective: To investigate the method to repair immedicable ulcer in skull cap in senile patient and the clinical effect of expanded bipedical axial flap in skull cap.

Methods: From September 2002 to June 2006, 5 patients with immedicable and chronic ulcer in skull cap were treated. All patients were males, aging 55-76 years.

[Histologic classification and prognostic implication of pseudomyxoma peritonei].

Objective: To clarify the various diagnostic connotations of pseudomyxoma peritonei (PMP) and to study their prognostic implications.

Methods: Clinicopathologic features and follow-up data of 40 patients with PMP diagnosed in The General Hospital of PLA were retrospectively reviewed. The cases were histologically classified into 3 subcategories: disseminated peritoneal adenomucinosis (DPAM), peritoneal mucinous carcinomatosis (PMCA), and PMCA with intermediate or discordant features (PMCA-I/D).

Apopotic gene Bax expression in carotid plaque.

The expression of BAX in carotid atherosclerosis and its regulation is far from defined. Objectives To investigate BAX expression in stable/fibrous and instable/vulnerable carotid plaque and its clinical significance. Methods Twenty-five cases of carotid plaque specimens obtained from endarterectomy were divided into two groups, stable/fibrous 14 cases, vulnerable/instable 11 cases; aortic artery and its branches from hepatic transplantation donors 6 case as control.

Clinical observation on treatment of hyperplasia of mammary gland by Lirukang Granule.

Objective: To explore the efficacy and mechanism of Lirukang Granule in treating hyperplasia of mammary gland (HMG).

Methods: One hundred patients with HMG were randomly assigned to two groups, 50 in each group. The patients in the treated group were orally administered with LRKG thrice a day, one package each time, and those in the control group were given orally Rukuaixiao Tablet thrice a day, 4 tablets each time.

[Clinical application of keratoprosthesis for corneal opacity caused by chemical burn unsuitable for keratoplasty].

Objective: To evaluate the results of keratoprosthesis for eyes with complicated corneal opacities and unsuitable for keratoplasty due to the chemical burn.

Methods: Twenty-eight keratoprosthesis were implanted in 28 patients with bilateral blindness. Preoperative visual acuity (VA) was light perception in all operated eyes.

[The interactions of stromal-epithelial in a model of co-culture in the rabbit cornea].

Objective: The purpose of the study is to produce a reconstructed cornea including epithelia and stroma by tissue engineering. The reconstructed tissue may provide a physiologic model for the investigations of interaction between corneal epithelial cells and keratocytes.

Methods: Epithelial cells and keratocytes were isolated from rabbit corneas and cultured on plastic substrates in vitro.

[LRP15 gene promoter region methylation and its expression in acute leukemia].

To investigate the relationship between LRP15 gene promoter region methylation and its gene expression in acute leukemia patients, the status of LRP15 gene promoter region methylation was detected by MS-PCR and the gene expression was detected by RT-PCR in bone marrow samples from leukemia patients. The results indicated that the LRP15 gene expression was 47.6% in complete remission (CR) patients and 16.

[Study on relationship between the C-V curve of the source points of 12 channels and cold and heat syndromes of channels].

Objective: To observe the relationship between C-V curve of the source points of 12 channels and cold Eighty patients with cold or heat syndrome were examined with Cowital Human Meor heat syndrome.

Methods: ridian Diagnosing and Analyzing System, which can achieve quantitative analysis on C-V curve from the source points of 12 channels, and additional 40 healthy people were used as control group.

Results: The relationship is close between the curvature of the C-V curves of the source points of the meridians and cold-heat syndromes of meridians.

Profiling of genes differentially expressed in a rat of early and later gestational ages with high-density oligonucleotide DNA array.

The early gestational fetus heals dermal wounds rapidly and scarlessly. This phenomenon appears to be intrinsic to fetal skin and is probably modulated by interplay of many genes. We ventured to study differences in gene expression between earlier gestational skin (EGS) and later gestational skin (LGS) with the aid of high-density oligonucleotide DNA array to explore the molecular mechanism underlying scarless healing.

[Hepatosplenic gammadelta T cell lymphoma and its relationship with Epstein-Barr virus infection].

To explore the clinical and pathological characteristics of hepatosplenic gammadelta T-cell lymphoma and its relationship with Epstein-Barr virus infection, the clinical features of a 9-year-old girl with hepatosplenic gammadelta T-cell lymphoma were investigated, the smears of bone marrow was stained with Wright' s stain, biopsies of bone marrow and liver specimen were embedded in plastic and sliced about 4 microm in thickness and routinely stained with HE staining, the immunohistochemical staining was used to mark the tumor cells, and EBER probes were used to detect Epstein-Barr virus RNA. The results showed that the girl presented with prolonged fever, anemia, thrombocytopenia, hepatosplenomegaly, chronic active Epstein-Barr virus infection, and elevated levels of serum ferritin and lactate dehydrogenase. Bone marrow aspirate revealed the infiltration of atypical lymphocytes in the bone marrow stroma.

[Effect of ubiquitin-proteasome pathway on inflammatory reaction in intestine and its barrier function in rats with postburn sepsis].

Objective: To study the effect of ubiquitin-proteasome pathway inhibition on intestinal nuclear factor-KappaB (NF-KappaB) activity and tumor necrosis factor-alpha (TNF-alpha) release as well as plasma diamine oxidase (DAO) activity in rats with postburn sepsis.

Methods: Rats were subjected to 30% total body surface area (TBSA) full-thickness scald injury, followed by intraperitoneal injection of lipopolysaccharide (LPS) to mimic postburn sepsis. Sixty Wistar rats were randomly divided into normal control group, sepsis group, sepsis with proteasome inhibitor N-Acetyl leucinyl leucinyl norleucinal (ALLN) treatment group and sepsis with NF-KappaB inhibitor pyrrolidine dithiocarbamate (PDTC) treatment group.

Potentiality of mesenchymal stem cells in regeneration of sweat glands.

Sweat glands play some key roles in homeostasis maintenance and body temperature regulation. In full-thickness burn wounds, sweat glands cannot regenerate to form their three-dimensional organization via the division and terminal differentiation of sweat gland cells. However, the plasticity of mesenchymal stem cells (MSCs) may offer the hope and potentiality to regenerate sweat glands after severe burn injury.