[Deepening the understanding of the diagnostic system and therapeutic strategy for burn sepsis].

Burn patients with large area of skin defect are prone to cause wound infection, severe fluid loss, and hypermetabolism, etc, which lead to sequential dysfunction of multiple systems and easily induce severe systemic infections and sepsis. At present, sepsis has been the leading causes of death in severe burn patients, and its early diagnosis and timely treatment are critical for successful treatment of patients. As burn sepsis has unique pathophysiological characteristics, the diagnostic criteria for general sepsis lack specificity for burn sepsis.

Characterization of alternative splicing events and prognostic signatures in breast cancer.

Background: Breast cancer (BRCA) is one of the most common cancers worldwide. Abnormal alternative splicing (AS) frequently observed in cancers. This study aims to demonstrate AS events and signatures that might serve as prognostic indicators for BRCA.

Bone Mesenchymal Stem Cells Contribute to Ligament Regeneration and Graft-Bone Healing after Anterior Cruciate Ligament Reconstruction with Silk-Collagen Scaffold.

Anterior cruciate ligament (ACL) reconstruction was realized using a combination of bone mesenchymal stem cells (BMSCs) and silk-collagen scaffold, and an in vivo evaluation of this combination was performed. By combining type I collagen and degummed silk fibroin mesh, silk-collagen scaffolds were prepared to simulate ligament components. BMSCs isolated from bone marrow of rabbits were cultured for a homogenous population and seeded on the silk-collagen scaffold.

Complications of thoracoscopic talc insufflation for the treatment of malignant pleural effusions: a meta-analysis.

Background: Talc pleurodesis is an effective treatment for malignant pleural effusions (MPEs). This study was designed to estimate complication rates of thoracoscopic talc insufflation.

Methods: Literature search was conducted in electronic databases and studies were selected if they reported complication rates of thoracoscopic talc insufflation in cancer patients with MPEs.

An Efficacy and Safety Study of Intra-arterial Recanalization of Occluded Ophthalmic Arteries in Patients with Monocular Blindness Caused by Injection of Hyaluronic Acid in Facial Tissues.

Propose: To evaluate the efficacy and safety of recanalizing hyaluronic acid-occluded retinal central artery by intra-arterially infusing hyaluronidase.

Methods: Between December 2015 and December 2016, 30 patients with monocular blindness due to the injection of hyaluronic acid in facial tissue underwent ophthalmic artery recanalization. The outcome of the intra-arterial treatment was retrospectively reviewed.

[Effects of Janus kinase/signal transduction and activator of transcription 3 pathway inhibitor in skeletal muscle function in severely burned rats and its mechanism].

To observe the functional changes of skeletal muscle in severely burned rats, and to investigate the effects and possible mechanisms of Janus kinase/signal transducer and activator of transcription 3 (JAK/STAT3) pathway inhibitor in skeletal muscle function. The experiment research method was applied. One hundred and twenty male Wistar rats of 8-week-old were divided into sham injury group, simple burn group, and burn+JAK/STAT3 inhibitor group according to the random number table, with 40 rats in each group.

Lung adenocarcinoma and sequential antineutrophil cytoplasmic antibody-associated vasculitis: a case report.

The relationship between antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) and lung cancer remains unclear. A 66-year-old man presented with pulmonary nodules. Histological examination of a specimen from computed tomography-guided percutaneous transthoracic biopsy revealed adenocarcinoma.

[Research advances on application of single-cell RNA sequencing in islet cell biology].

After the first single-cell RNA sequencing of human islet in 2016, a large number of analysis of RNA sequencing at single-cell level on mouse and human islets emerged, which has brought new progress to the research of islet cell biology. It has been proved that single-cell RNA sequencing can characterize rare types of endocrine cells, find the cellular heterogeneity of typical endocrine cells and new cell subtypes, analyze the differences of endocrine cells among species, and describe different states of various types of islet cells in developmental processes and metabolic diseases more accurately. Although the detection efficiency of single-cell RNA sequencing for low abundance transcripts is relatively low at present, with the improvement of technology and the progress of analytical methods, this technology will undoubtedly become a powerful tool to explore the cellular heterogeneity, development, and biological characteristics of metabolic diseases.

[Pathological diagnosis of Danon disease by endomyocardial biopsy].

O To investigate the clinicopathological features and differential diagnosis of Danon disease. Two cases of Danon disease were selected from Beijing Anzhen Hospital Affiliated to Capital Medical University from January 2019 to December 2019. The clinical history, histological, immunohistochemical, ultrastructural and gene mutation analysis were collected.

Clinical practice guidelines for the management of adult diffuse gliomas.

To follow the revision of the fourth edition of WHO classification and the recent progress on the management of diffuse gliomas, the joint guideline committee of Chinese Glioma Cooperative Group (CGCG), Society for Neuro-Oncology of China (SNO-China) and Chinese Brain Cancer Association (CBCA) updated the clinical practice guideline. It provides recommendations for diagnostic and management decisions, and for limiting unnecessary treatments and cost. The recommendations focus on molecular and pathological diagnostics, and the main treatment modalities of surgery, radiotherapy, and chemotherapy.

Identification of Hub Genes Associated With Hepatocellular Carcinoma Using Robust Rank Aggregation Combined With Weighted Gene Co-expression Network Analysis.

Background: Bioinformatics provides a valuable tool to explore the molecular mechanisms underlying pathogenesis of hepatocellular carcinoma (HCC). To improve prognosis of patients, identification of robust biomarkers associated with the pathogenic pathways of HCC remains an urgent research priority.

Methods: We employed the Robust Rank Aggregation method to integrate nine qualified HCC datasets from the Gene Expression Omnibus.

Knockdown of CircCRIM1 Inhibits HDAC4 to Impede Osteosarcoma Proliferation, Migration, and Invasion and Facilitate Autophagy by Targeting miR-432-5p.

Background: Circular RNAs (circRNAs) serve for a genre of considerable modulatory molecules that have been largely researched in human cancers. However, the contribution of circRNA cysteine-rich transmembrane bone morphogenetic protein regulator 1 (circCRIM1) to osteosarcoma (OS) is completely unclear.

Methods: All the RNA levels were examined via quantitative real-time polymerase chain reaction (qRT-PCR).

LncRNA-SNHG7 interferes with miR-34a to de-sensitize gastric cancer cells to cisplatin.

Gastric cancer (GC) remains poor prognosis and survival issues due to the resistance of chemotherapies, such as cisplatin. The long non-coding RNA small nucleolar RNA host gene 7 (lncRNA-SNHG7) is known as an oncogenic molecule in diverse cancers. Here, we demonstrate that SNHG7 was significantly upregulated in gastric cancer and positively correlated with cisplatin resistance of gastric cancer cells that SNHG7 was significantly upregulated in cisplatin resistant cells.

Perivascular epithelioid cell tumor (PEComa) of the kidney: an overview of its management and outcomes.

Perivascular epithelioid cell tumor (PEComa) is a rare mesenchymal tumor with unique histological and immunohistochemical characteristics. Malignant renal cell carcinoma is even rarer and its biological behavior is still not clear. We report here a case of malignant PEComa in a male patient.

MiR-140-5p promotes osteogenic differentiation of mouse embryonic bone marrow mesenchymal stem cells and post-fracture healing of mice.

MiR-140-5p is high expressed in normal fracture healing, but its specific role and mechanism in tissue-to-bone healing are rarely reported. Therefore, this study investigated the effects of miR-140-5p on tissue-to-bone healing. Clone formation experiment, flow cytometry, Alizarin Red S Staining and Oil Red O Staining were performed to investigate the biological characteristics of mouse embryonic bone marrow mesenchymal stem cells C3H10T1/2.

[Retrospective analysis of 2 997 inpatients with skin and soft tissue injuries].

To analyze the epidemiological characteristics and the related information on the diagnosis and treatment of inpatients with skin and soft tissue injuries. The medical records of inpatients with skin and soft tissue injuries who were admitted to the Fourth Medical Center of PLA General Hospital (hereinafter referred to as the author's unit) from January 1, 2014 to December 31, 2018, conforming to the study criteria, were retrospectively analyzed. The indexes included inpatients'gender, age, wound course, underlying disease, wound type, wound site, and wound microbiological culture result, methods and outcome of treatment, type of medical payment, hospital day, and hospitalization cost.

[Ten cases of wound hemostasis with glove bandaging in hand skin grafting].

From July 2018 to April 2019, 10 patients (8 males and 2 females, aged 5 to 53 years) who need skin grafting with 17 hands were admitted to the Fourth Medical Center of PLA General Hospital. The method of glove bandaging was used for wound hemostasis in skin grafting. After the initial hemostasis, sterile latex gloves were used as the inner cover of hand, and then pressure-wrapped with gauze.

[New concept of chronic wound healing: advances in the research of wound management in palliative care].

With the aggravation of aging of global population and increase of incidence of various chronic diseases and injuries, patients with chronic wounds due to different causes (malnutrition, abnormal metabolism, compression, infection, tumor, etc.) have grown. Especially in some patients at the end of life, chronic wounds have the characteristics of difficult to heal and even unable to heal.

[Establishment and application of a clustered management plan for pulmonary care of massive burn casualties].

To establish a clustered management plan for pulmonary care of massive burn casualties (hereinafter referred to as the clustered management plan for pulmonary care), and to explore its application effects. (1) A clustered care intervention group was established, including the medical and nursing staff from the Department of Burns and Plastic Surgery, Department of Respiratory Medicine, and Department of Infection Control at the Fourth Medical Center of PLA General Hospital (hereinafter referred to as our hospital). Four major links, including pulmonary care assessment, chest and lung physical therapy, artificial airway management, and specialized infection control were sorted out according to the key points and difficulties in pulmonary care for massive burn casualties.

Downregulation of LINC00707 promotes osteogenic differentiation of human bone marrow‑derived mesenchymal stem cells by regulating DKK1 via targeting miR‑103a‑3p.

Human bone marrow‑derived mesenchymal stem cells (HBMSCs) have the potential of multidirectional differentiation and self‑renewal, which is important for the formation of human bone. It has been reported that long non‑coding RNAs (lncRNAs) serve important roles in HBMSC osteogenic differentiation. The current study aimed to investigate the roles of long intergenic non‑protein coding RNA 00707 (LINC00707) and microRNA (miR)‑103a‑3p in the osteogenic differentiation of HBMSCs.

[Clinical strategies for preservation of the exposed implant in chronic wounds and wound repair].

To explore the clinical strategies for preservation of the exposed implant in chronic wounds and wound repair. From January 2016 to January 2019, totally 8 patients (4 males and 4 females, aged 10 to 73 years) sustaining postoperative chronic wounds with exposed implants were admitted to the Fourth Medical Center of PLA General Hospital. There were 2 cases of abdominal patch exposure after abdominal trauma surgery, 2 cases of titanium plate exposure post craniocerebral surgery, 3 cases of internal fixator exposure post orthopedic surgery, and 1 case of cerebrospinal fluid drainage tube exposure after craniocerebral surgery.

[Selection and effects of flap/myocutaneous flap repair methods for the defect after perineum tumor resection].

To explore the selection and effects of flap/myocutaneous flap repair methods for the defect after perineum tumor resection. From January 2011 to February 2017, 31 patients with vulvar tumor who were admitted to Hunan Cancer Hospital underwent repair of wound after tumor resection with various flaps/myocutaneous flaps. The patients were composed of 5 males and 26 females, aged 39-76 years, with 27 vulvar cancer and 4 Paget's disease in primary diseases.

[Some thoughts on the coordinated development of burn department and wound repair department].

On November 29, 2019, in order to strengthen the management of the diagnosis and treatment of the chronic refractory wounds, the National Health Commission released a notice that requires the qualified medical institutions in China to establish wound repair department. To ease the concern that the establishment of wound repair department could hinder the construction and development of burn discipline, the authors put forward their views based on the necessity of establishing wound repair department, the space for the respective development of burn department and wound repair department, and how to coordinate the development of burn department and wound repair department. It is hoped that this paper would be used as a reference by doctors in both fields of burn care and wound repair.