Ku70 targets BRD3-MYC/Cyclin D1 axis to drive hepatocellular carcinoma progression.

Hepatocellular carcinoma (HCC) is a common cancer characterized by robustly proliferative and metastatic capabilities. Bromodomain-containing proteins are critical to the development of diverse diseases via regulating cell proliferation, differentiation, and death. However, the role of Bromodomain-containing protein 3 (BRD3) in HCC is elusive.

Differential Response to Cisplatin between Co-cultured Cells and Pure Cultured Cells Based on Single-cell RNA Sequencing of Three-dimensional-cultured Breast Cancer Cells.

Objective: The current study aimed to develop an experimental approach for the direct co-culture of three-dimensional breast cancer cells using single-cell RNA sequencing (scRNA-seq).

Methods: The following four cell culture groups were established in the Matrigel matrix: the untreated Michigan Cancer Foundation (MCF)-7 cell culture group, the MCF-7 cell culture plus cisplatin group, the untreated co-culture group, and the cell co-culture plus cisplatin group. For cell co-culture, MCF-7 cells, human mammary fibroblasts, and human umbilical vein endothelial cells were mixed at a ratio of 1:1:1.

Ubiquitin-specific protease 1 facilitates tumor immune escape from natural killer cells and predicts the prognosis in small cell lung cancer.

Objective: Small cell lung cancer (SCLC) is commonly recognized as the most fatal lung cancer type. Despite substantial advances in immune checkpoint blockade therapies for treating solid cancers, their benefits are limited to a minority of patients with SCLC. In the present study, novel indicators for predicting the outcomes and molecular targets for SCLC treatment were elucidated.

Effect of perioperative dexmedetomidine on recovery of postoperative gastrointestinal function in patients with general anesthesia: a systematic review and meta-analysis.

Background: There is controversy surrounding the influence of dexmedetomidine on the recovery of postoperative gastrointestinal dysfunction in patients under general anesthesia. The main purpose of this meta-analysis is to evaluate the effect of dexmedetomidine administration during the perioperative period on the recovery of gastrointestinal function in patients under general anesthesia.

Methods: A systematic review and meta-analysis with trial sequential analysis was performed to identify randomized controlled trials comparing dexmedetomidine administration with placebo for the recovery of gastrointestinal function.

The predictive value of thyroid hormone sensitivity parameters for cervical lymph node metastasis in patients with differentiated thyroid cancer.

Objective: To comprehensively investigate the predictive value of thyroid hormone sensitivity parameters for cervical lymph node metastasis in patients diagnosed with differentiated thyroid cancer (DTC) undergoing total thyroidectomy and neck lymph node dissection.

Methods: A retrospective cohort study was conducted involving patients diagnosed with DTC and evaluated for cervical lymph node metastasis. Relevant demographic, tumour, lymph node and thyroid hormone sensitivity parameter data were extracted from medical records and laboratory reports.

Utilizing combined quantitative multiparametric MRI as potential biomarkers for improved early-stage parkinson's disease diagnosis.

Background: Identifying Parkinson's disease (PD) during its initial phases presents considerable hurdles for clinicians.

Purpose: To examine the feasibility and efficacy of a machine learning model based on quantitative multiparametric magnetic resonance imaging (MRI) features in identifying early-stage PD.

Methods: We recruited 33 participants, including 19 with early-stage PD, 14 with advanced-stage PD and 20 healthy control subjects.

Single-cell transcriptomes of dissecting the intra-tumoral heterogeneity of breast cancer microenvironment.

Objective: To investigate the mechanism by which heterogeneity in breast cancer developed and acted in single-cell transcriptomes.

Methods: The composition of breast cancer based on the single-cell transcriptomes of 54,055 high-quality cells from clinical specimens of 4 malignant and 4 non-malignant patients were investigated.

Results: We identified six common expression programs and six subtype-specific expression programs form malignant epithelial cells.

PEARL: A Multicenter Phase 2 Study of Lorlatinib in Patients with ALK-Rearranged NSCLC and Central Nervous System Disease.

Background: Patients with ALK-rearranged non-small cell lung cancer (ALK+ NSCLC) with symptomatic brain (BM) and leptomeningeal (LM) metastases are underrepresented in clinical trials due to poor performance status. Additionally, the need for improved and validated assessment criteria for evaluating central nervous system (CNS) response remains critical. Lorlatinib has demonstrated systemic activity in patients with ALK+ NSCLC.

Intelligent bacteria-targeting ZIF-8 composite for fluorescence imaging-guided photodynamic therapy of drug-resistant superbug infections and burn wound healing.

Infected burn wounds are characterized by persistent drug-resistant bacterial infection coupled with an inflammatory response, impeding the wound-healing process. In this study, an intelligent nanoparticle system (CCM+TTD@ZIF-8 NPs) was prepared using curcumin (CCM), an aggregation-induced emission luminogens (TTD), and ZIF-8 for infection-induced wound healing. The CCM+TTD@ZIF-8 NPs showed multiple functions, including bacteria targeting, fluorescence imaging and pH response-guided photodynamic therapy (PDT), and anti-inflammatory.

Efficacy of local-regional radiotherapy in de novo metastatic nasopharyngeal carcinoma patients receiving chemo-immunotherapy: A multicenter, propensity score matching study.

Background: To evaluate the efficacy of local-regional radiotherapy (LRRT) in de novo metastatic nasopharyngeal carcinoma (dm NPC) patients receiving chemo-immunotherapy as first-line treatment and select the beneficiaries from LRRT.

Methods And Materials: M1-NPC patients receiving platinum-based chemo-immunotherapy with or without LRRT from four centers were included in this study. The propensity score matching (PSM) analysis was employed to balance the baseline characteristics between the LRRT and non-LRRT groups.

Cervical cancer screening by biomarker-free Serum-SERS technique: A three-principal-substrate approach.

The complexity of serum constituents and the lack of knowledge on biomarkers bring obstacles for reliable cancer screening with the limited information obtained by surface-enhanced Raman scattering (SERS) on a single substrate. We report here an extremely high-accurate approach for cancer screening by biomarker-free serum-SERS technique which employs three principal SERS substrates that are of specially designed surface properties. With three substrates rich information on serum can be obtained from SERS spectra, of which quadratic discriminant analysis (QDA) yields excellent classifications of serum samples independently.

A Deep Learning Network for Accurate Retinal Multidisease Diagnosis Using Multiview Fusion of En Face and B-Scan Images: A Multicenter Study.

Purpose: Accurate diagnosis of retinal disease based on optical coherence tomography (OCT) requires scrutiny of both B-scan and en face images. The aim of this study was to investigate the effectiveness of fusing en face and B-scan images for better diagnostic performance of deep learning models.

Methods: A multiview fusion network (MVFN) with a decision fusion module to integrate fast-axis and slow-axis B-scans and en face information was proposed and compared with five state-of-the-art methods: a model using B-scans, a model using en face imaging, a model using three-dimensional volume, and two other relevant methods.

[Therapeutic efficacy of ear endoscopic surgery for middle ear cholesterol granuloma].

This study aimed to investigate the surgical efficacy of middle ear cholesterol granuloma using otoendoscopy. A total of 23 patients (9 males and 14 females) aged (38±18) years who were diagnosed with middle ear cholesterol granuloma at the Otology Medical Center, the First People's Hospital of Foshan from May 2020 to August 2023 were retrospectively analyzed. Based on clinical characteristics of the lesions, the patients were categorized into retention type and granulation type; the latter was further subdivided into localized and extensive types.

[Expert consensus on endoscopic surgery for the pediatric middle ear cholesteatoma based on the classification of cholesteatoma location].

Pediatric middle ear cholesteatoma is an aggressive keratotic lesion caused by abnormal proliferation of keratinised squamous epithelium in the temporal bone, with the characteristics of high postoperative recurrence rate, poor hearing effect and poor quality of life. Endoscopic surgery is able to deal with the majority of pediatric middle ear cholesteatoma cases with the features of minimal trauma and wide surgical field. However, endoscopic surgical treatment still has problems in standardization.

PPIH Expression Correlates with Tumor Aggressiveness and Immune Dysregulation in Hepatocellular Carcinoma.

Purpose: Hepatocellular Carcinoma (HCC) features a complex pathophysiology and unpredictable immunosuppressive microenvironment, which limit the effectiveness of traditional therapies and lead to poor patient outcomes. Understanding the immune characteristics of HCC is essential for elucidating the immune microenvironment and developing more effective treatments. This study investigates the role of Peptidyl-prolyl isomerase H (PPIH) in HCC by analyzing its expression, prognosis, methylation levels, and relationship with immune cell infiltration.

General anesthesia induces acute cell-free DNA methylation changes in peripheral blood.

Background: Short-term and long-term adverse events could occur after general anesthesia (GA) and the specific mechanism driving these effects has not yet been well-characterized. In this study, we aimed to evaluate the global effect of GA on DNA methylation in the cell-free DNA (cfDNA) of surgical lung-nodule patients.

Methods: This large retrospective cohort study enrolled 1,006 surgical lung nodule patients (529 pre-anesthesia, and 477 post-anesthesia).

Subanesthetic propofol alleviates chronic stress-induced anxiety by enhancing VTADA neurons' activity.

Anxiety, a common mental disorder, imposes significant clinical and economic burdens. Previous studies indicate that propofol has anxiolytic effects at anesthetic doses. However, the risks associated with general anesthesia limit its application in anxiety treatment.

Peripheral immune characteristics and subset disorder in reproductive females with endometriosis.

Pathogenesis of endometriosis (EN) is still unknown, but growing evidence suggests that immune regulation may be important, and the pattern of peripheral immune changes in reproductive women with EN has yet to be fully explored. In this study, we conducted a comprehensive and systematic analysis of immune cell subsets within T cells, B cells, NK cells, and γδ T cells in peripheral blood (PB) samples from women with EN, women with uterine fibroids (UF) but without EN (UF-alone), and healthy controls using multi-parameter flow cytometry. Our findings revealed that UF, a common comorbidity of EN, exhibited similar peripheral immune features to EN, particularly in T cell and B cell immunity.

Noninvasive identification of proliferative hepatocellular carcinoma on multiphase dynamic CT: quantitative and LI-RADS lexicon-based evaluation.

Objective: To identify proliferative hepatocellular carcinoma (HCC) preoperatively using quantitative measurements combined with the updated standard 2021 LI-RADS universal lexicon-based qualitative features on multiphase dynamic CT (MDCT).

Methods: We retrospectively analyzed 273 patients (102 proliferative HCCs) who underwent preoperative MDCT with surgically confirmed HCC in two medical centers. Imaging features were evaluated according to the updated 2021 LI-RADS universal lexicon, and quantitative measurements were analyzed.

A predictive model of treatment effectiveness of refractory peritoneal dialysis-related peritonitis in patients with peritoneal dialysis: a single-center observational study in South China.

Background: To prevent loss of peritoneal function caused by persistent abdominal inflammation, the guidelines recommend early extubation in patients with refractory peritoneal dialysis (PD)-associated peritonitis (rPDAP). In attempt to pinpoint high-risk patient cohorts that did not respond to treatment for refractory peritonitis, we created a model to predict the effectiveness of peritonitis treatment.

Methods: This observational cohort study included PD patients from 1 January 2011 to 31 December 2020.

Chronic kidney disease and thyroid hormones.

Introduction: Thyroid dysfunction is prevalent in chronic kidney disease (CKD) patients and significantly impacts renal outcomes and mortality. This study investigated the associations between thyroid function and clinical outcomes, and also the therapeutic effects of thyroid hormone replacement therapy (THRT) in CKD patients.

Methods: We conducted a retrospective cohort study using data from the China Renal Data System.

Analysis of Prognostic Factors in Patients with Sudden Sensorineural Hearing Loss.

To explore the relevant risk factors of poor prognosis in patients with sudden sensorineural hearing loss (SSNHL), constructing prognostic prediction model, formulating effective treatment strategies, and providing reliable theoretical basis for clinical prevention of and treatment for poor prognosis in SSNHL. A retrospective analysis was conducted on the clinical data of 123 patients with SSNHL who received treatment at Zhongxian People's Hospital of Chongqing from March 2022 to March 2024. According to the prognosis, they were divided into an effective group (68 cases with an average hearing threshold increase of ≥15 dB, as good prognosis) and an ineffective group (55 cases with an average hearing threshold increase of <15 dB, as poor prognosis).

Dual-Functionalized Injectable pH-Responsive Hydrogels Loaded with Magnesium Peroxide for the Treatment of Implant-Associated Infection.

Implant-associated infections frequently complicate orthopedic surgeries, resulting in challenging issues. The current therapy of antibiotic treatment and surgical debridement often leads to drug resistance and bone defect. The development of pH-responsive antimicrobial and pro-osteogenic materials is a promising approach to controlling infections and repairing infected bone defects, especially given the weakly acidic pH of the bacterial infection area.

Development and Validation of Multiparametric MRI-based Interpretable Deep Learning Radiomics Fusion Model for Predicting Lymph Node Metastasis and Prognosis in Rectal Cancer: A Two-center Study.

Rationale And Objectives: To develop interpretable machine learning models that utilize deep learning (DL) and radiomics based on multiparametric Magnetic resonance imaging (MRI) to predict preoperative lymph node (LN) metastasis in rectal cancer.

Materials And Methods: This retrospective study involved 286 cancer patients confirmed by histopathology from center 1 (Training set) and 66 patients from center 2 (External test set). Radiomics features were extracted from T2-weighted imaging (T2WI) and diffusion-weighted imaging (DWI) sequences, whereas DL features were obtained using four models: MobileNet-V3-large, Inception-V3, ResNet50, and VGG16.

AI-based fingerprint index of visceral adipose tissue for the prediction of bowel damage in patients with Crohn's disease.

The fingerprint features of visceral adipose tissue (VAT) are intricately linked to bowel damage (BD) in patients with Crohn's disease (CD). We aimed to develop a VAT fingerprint index (VAT-FI) using radiomics and deep learning features extracted from computed tomography (CT) images of 1,135 CD patients across six hospitals (training cohort,  = 600; testing cohort,  = 535) for predicting BD, and to compare it with a subcutaneous adipose tissue (SAT)-FI. VAT-FI exhibited greater predictive accuracy than SAT-FI in both training (area under the receiver operating characteristic curve [AUC] = 0.