Effect of daunorubicin on acute promyelocytic leukemia cells using nuclear magnetic resonance spectroscopy-based metabolomics.

The aim of this study was to determine several key metabolites as potential biomarkers of daunorubicin (DNR) treatment of acute promyelocytic leukemia (APL) using APL blasts and NB4 cells. Samples which were obtained from 16 newly diagnosed APL patients and human APL NB4 cell lines were exposed to increasing concentrations of DNR (0 μM, 0.1 μM, 0.

IRGM1 enhances B16 melanoma cell metastasis through PI3K-Rac1 mediated epithelial mesenchymal transition.

Melanoma is one of the most aggressive skin cancers and is well known for its high metastatic rate. Studies have shown that epithelial mesenchymal transition (EMT) is essential for melanoma cell metastasis. However, the molecular mechanisms underlying EMT are still not fully understood.

Inhibition of hepatitis B virus cccDNA by siRNA in transgenic mice.

The elimination of viral covalently closed circular DNA (cccDNA) from the nucleus of infected hepatocytes is an obstacle to achieving sustained viral clearance during antiviral therapy of chronic hepatitis B virus (HBV) infection. The aim of our study was to determine whether treatment with siRNA is able to suppress viral cccDNA amplification using a HBV-transgenic mice model. The experimental results revealed that siRNAs can serve as efficient alternative anti-HBV agents, because they showed better inhibitory effect on viral replication and antigen expression in transgenic mice.

siRNA combinations mediate greater suppression of hepatitis B virus replication in mice.

Hepatitis B virus (HBV) infection is a major world-wide health problem. The major obstacles for current anti-HBV therapy are the low efficacy and the occurrence of drug resistant HBV mutations. Recent studies have demonstrated that combination therapy can enhance antiviral efficacy and overcome shortcomings of established drugs.

[Analysis of prognosis factors of idiopathic sudden hearing loss].

Objective: To study the prognosis factors of idiopathic sudden hearing loss.

Method: The clinical data of 165 patients with idiopathic sudden hearing loss were retrospectively studied and the computer software was used to investigate the relevant effects on prognosis.

Result: The prognosis of patients who had been treated within 14 days under the age of 55 were better.

[Analysis on non-invasive fungal rhinosinusitis for 59 cases by endoscopic sinus surgery].

Objective: To explore the etiology and clinical features of non-invasive fungal rhinosinusitis and to evaluate the methods and curative effects of endoscopic sinus surgery.

Method: The denial and follow-up data of 59 patients with non-invasive fungal rhinosinusitis were retrospectively analyzed.

Result: Following up for 2-5 years, no complications were observed.