MicroRNA-34a promotes MICB expression in hepatocytes.

MicroRNA-34a (miR-34a) behaves as a tumor suppressor by decreasing the expression of oncogenes involved in multiple carcinogenic pathways. Intravenous delivery of miR-34a mimics has been investigated in clinical trials as a potential treatment for advanced cancers; however, the effect of miR-34a on cancer immune surveillance is controversial. In the current study, we found that miR-34a plays a dual role in the regulation of major histocompatibility complex class I-related sequence B (MICB) protein, a ligand of the NKG2D receptor.

[Analysis of risk factors of infection for complex tibial plateau fractures after operation].

Objective: To analyze the risk factor of infection for complex tibial plateau fractures after operation.

Methods: Totally clinical data of 293 patients with complex tibial plateau fractures underwent open reduction and internal fixation were retrospectively analyzed from September 2010 to March 2015, including 199 males and 94 females, ranging in age from 17 to 80 years old with an average of 47.3 years old.

LC-MS/MS analysis and pharmacokinetic study on five bioactive constituents of Tanreqing injection in rats.

Tanreqing injection (TRQ), a well-known traditional Chinese medicine formula, is commonly used to treat respiratory diseases. In the present study, a rapid, selective, and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to simultaneously determinate the plasma contents of 5 major constituents of TRQ, including chlorogenic acid (CHA), caffeic acid (CFA), baicalin (BA), ursodeoxycholic acid (UDCA) and chenodeoxycholic acid (CDCA) in rats after intravenous administration of TRQ. Chromatographic separation was performed on an Agilent Zorbax SB-C column (3.

Neuroprotective and antioxidant lanostanoid triterpenes from the fruiting bodies of Ganoderma atrum.

Five new lanostanoid triterpenes were isolated from the ethanol extract of the fruiting bodies of Ganoderma atrum. The structures of the isolated compounds were established based on 1D and 2D ((1)H-(1)H COSY, HMQC, and HMBC) NMR spectroscopy, in addition to high resolution mass spectrometry. The isolated compounds were tested in vitro for neuroprotective activities against 6-OHDA-induced cell death in SH-SY5Y cells and radical scavenging activities.

Early diagnosis and treatment of trauma in knee joints accompanied with popliteal vascular injury.

Objective: The objective of the present study was to investigate the early diagnosis and treatment of trauma in the knee joints accompanied with popliteal vascular injury.

Methods: Fifteen cases of patients with trauma in knee joints accompanied with popliteal vascular injury. These patients included 8 males and 6 females between the ages of 27 and 62, the average age being 39.

Percutaneous dilatational tracheostomy for ICU patients with severe brain injury.

Objective: To sum up our experience in percutaneous dilatational tracheostomy (PDT) in ICU patient with severe brain injury.

Methods: Between November 2011 and April 2014, PDTs were performed on 32 severe brain injury patients in ICU by a team of physicians and intensivists. The success rate, efficacy, safety, and complications including stomal infection and bleeding, paratracheal insertion, pneumothorax, pneumomediastinum, tracheal laceration, as well as clinically significant tracheal stenosis were carefully monitored and recorded respectively.

An efficacy analysis of surgical timing and procedures for high-energy complex tibial plateau fractures.

Objective: To identify the most effective treatment for application to high-energy complex plateau fractures.

Methods: 125 patients with closed high-energy complex tibial plateau fractures who had been surgically treated between 2003 and 2010 were reviewed retrospectively. Five procedure methods were studied (e.

[Case-control study on operative treatment for complex tibial plateau fracture].

Objective: To explore optimal choice of surgical treatment and operative approach for closed complex tibial plateau fractures and its influencing factors.

Methods: From January 2003 to January 2011, 95 patients with closed complex tibial plateau fractures were estimated Schatzker V and Vl, and treated with three different surgical methods. The methods included single plate through anterolateral incision (Group A, 22 cases), double plates through inside and outside incisions (Group B, 36 cases), and double plates through antero-midline incisions (Group C, 37 cases).

[Case-control study on MDCT and MRI for the diagnosis of complex fractures of tibial plateau].

Objective: To evaluate the clinical value of MDCT and MRI in the diagnosis and treatment of complex fractures of tibial plateau.

Methods: From March 2004 to January 2009,71 patients with complex fractures of tibial plateau estimated Schatzker III, V and VI were included in the study. Their X-ray films, MDCT, MRI were analyzed and compared.

Clinical study of reconstructing the medial malleolus with free grafting of fibular head composite tendon bone flap.

Objective: To explore new surgical procedure for repairing and reconstructing medial malleolus and soft tissue defect.

Methods: According to the size of medial malleolus and composite soft tissue defect, vascular fibular head composite tendon bone flap free grafting using anterior tibial recurrent vessel as the perforating branch was designed to reconstruct the medial malleolar saddle and triangular ligament, and composite back broadest muscle flap free grafting was designed to repair the medical malleolar wound surface. From January 2000 to December 2006, the technique was used in 5 male patients who were injured by machine or in traffic accidents, causing bone and soft tissue defect.

[Combined treatment of refractory decubitus ulcers].

Objective: To summarize the clinical experience in the treatment of refractory decubitus ulcers.

Methods: From May 1998 to March 2005, 22 patients with decubitus ulcers (29 decubitus ulcers) were admitted, whose age was 36-92 years. The lesion size was 4 cm x 2 cm to 18 cm x 15 cm.