[Advances in the technology in modern rehabilitation of patients after burns].

With the rapid development of modern science and technology, various kinds of new therapy and technology have widely been used in rehabilitation medicine. This review summarizes those new therapies and principles in rehabilitation and their application during treatment of burn injury, in order to provide more ways and methods to improve the rehabilitation of mentation, motor function, and the quality of life of burn patients.

[Second operation after laser in situ keratomileusis].

Objective: To compare clinical results between the laser in situ keratomileusis (LASIK) surgery and the laser subepithelial keratomileusis (LASEK) surgery as the second operation after an unsuccessful LASIK surgery.

Methods: Thirty one patients (62 eyes) with refractive regression or under-correction after LASIK operation received the second surgery. Thirty eyes received LASIK surgery (group A) and 32 eyes received LASEK surgery (group B).

[Progress in studies of mechanism of anti-fibrosis traditional Chinese drugs and effective components].

The progress in the research of pharmacological mechanism of anti-fibrosis traditional Chinese drugs and the effective components is summarized. It's demonstrated by pharmacological experiments that anti-fibrosis traditional Chinese drugs can inhibit the cell proliferation, regulate the cytokines and ECM, and intervente in the signal transduction.

[The effect of aspirin on human's gammadeltaT cells killing digestive system tumor cell lines].

Aim: To explore the effect of aspirin on human's gammadeltaT cells killing digestive system tumor cell lines.

Methods: Use the isopentenyl pyrophosphate method to amplify human peripheral blood gammadeltaT cells in vitro. Various concentrations of aspirin were used to induce gammadeltaT cells and digestive system tumor cells SGC-7901, SW-1990, SW-480, SW-1116, LOVO cells lines, LDH assays was used to measure the cytotoxic activity of gammadeltaT cells, flow cytometry analysis the apoptosis percentage of before and after induced gammadeltaT cells and SGC-7901, SW-1990, SW-480, SW-1116, LOVO cell lines.

[Effects of glycyrrihizic acid and prednisone on pathological and ultrastructural changes of kidney in rats with chronic aristolochic acid nephropathy].

Objective: To investigate the effects of glycyrrihizic acid (GA) and prednisone on renal injury in chronic aristolochic acid nephropathy (AAN) rat model.

Methods: Ninety-eight male Wistar rats of SPF grade were randomly divided into 4 groups, the normal control group (n = 20), the GA group, the model group, and the prednisone group, 26 rats in each group. Rats in the latter 3 groups were made into AAN model by administration of aristolochic acid (AA, contained in extract of Caulis Aristolochiae Manshuriensis) 20 mg/(kg x d) by gastric gavage, and equal volume of drinking water was given to rats in the control group.

[Changes in serotonin and noradrenaline in hepatic encephalopathy as a result of liver failure in rat].

Objective: To investigate the changes in serotonin (5-HT) and noradrenaline (NA) in hepatic encephalopathy as a result of acute and chronic liver failure in rat.

Methods: One hundred and ten Sprague-Dawley (SD) rats were randomly divided into groups of normal control (n=20), experimental group of acute liver failure (ALF) encephalopathy (n=45), and experimental group of chronic liver failure (CLF) encephalopathy (n=45). Two dosages of thioacetamide (TAA) of 500 mg/kg were gavaged with an interval of 24 hours to reproduce ALF model.

[Effect of ubiquitin-proteasome pathway on inflammatory reaction in intestine and its barrier function in rats with postburn sepsis].

Objective: To study the effect of ubiquitin-proteasome pathway inhibition on intestinal nuclear factor-KappaB (NF-KappaB) activity and tumor necrosis factor-alpha (TNF-alpha) release as well as plasma diamine oxidase (DAO) activity in rats with postburn sepsis.

Methods: Rats were subjected to 30% total body surface area (TBSA) full-thickness scald injury, followed by intraperitoneal injection of lipopolysaccharide (LPS) to mimic postburn sepsis. Sixty Wistar rats were randomly divided into normal control group, sepsis group, sepsis with proteasome inhibitor N-Acetyl leucinyl leucinyl norleucinal (ALLN) treatment group and sepsis with NF-KappaB inhibitor pyrrolidine dithiocarbamate (PDTC) treatment group.

[Relationship between millimeter wave irradiation in pregnant mice and c-Fos protein expression in hippocampus and learning and memory functions in their offsprings].

Objective: To determine the threshold of millimeter wave irradiation for fetal injury in mice and the mechanism of decrease of learning and memory function in their offsprings and to verify whether the millimeter wave has the non-thermal effect.

Methods: Pregnant mice were irradiated by millimeter wave with frequencies of 37.4, 42.

[Relationship between nuclear factor-kappaB activity and kidney injury in rats with postburn sepsis].

Objective: To investigate the relationship between nuclear factor-KappaB (NF-KappaB) activity and kidney injury in rats with postburn sepsis.

Methods: Rats subjected to 30% full-thickness scald injury, followed by intraperitoneal injection of lipopolysaccharide (LPS), were used in the present study. Fifty-four Wistar rats were randomly divided into normal control group, postburn sepsis 1, 2, 6, 12, 24 hours groups, and sepsis with NF-KappaB inhibitor pyrrolidine dithiocarbamate (PDTC) treatment 1, 2, and 6 hour groups.

[Characteristic of medical support and health care for Shenzhou-5 and Shenzhou-6 astronauts at main landing site].

Objective: To investigate the characteristics of medical support and health care for the Shenzhou-5 and Shenzhou-6 astronauts at the main landing site, with special emphasis on the technical requirements for two astronauts during flight, in order to provide reference data for medical support in the battle field and disasters.

Methods: The data associated with accidental injuries of astronauts during the space flight collected from foreign nations and domestically were reviewed retrospectively. Based on the experience in medical support for Shenzhou-5 and Shenzhou-6 astronauts and the special environments of field operations, a scheme for first-aid and emergent treatment were drafted for a system of organization, prophylactic measurements, equipment and their effective implementation pending the test in the real situation.

[Apoptosis of neutrophil in peripheral blood in experimental sepsis].

Objective: To evaluate the changes in apoptosis of neutrophil in peripheral blood in sepsis in rats.

Methods: The rat sepsis model was reproduced by cecum ligation and puncture (CLP). One hundred and forty-four rats were randomly divided into normal control group, sham operation group and 2, 6, 12, 24, 48, 72 hours after CLP groups.

[Tissue flap transferring for wound repair of the clavicle].

Objective: To investigate the result of tissue flap transferring for wound repair of the clavicle.

Methods: From 1994 to 2000, 3 patients (1 with clavicle osteosynthesis, 1 with chronic clavicle osteomyelitis, and 1 with radioactive ulcer in clavicular region accompanied by chronic osteomyelitis of clavicle) were reconstructed with turnover adipofascial flap, myocutaneous flap of pectoris, and myocutaneous flap of latissimus dorsal respectively. The outcome was observed.

[Effect of 3-dimensional conformal hypofractionated high-dose radiotherapy combined with chemotherapy for non-small-cell lung cancer: a clinical observation].

Objective: To evaluate the clinical therapeutic effect of three-dimensional conformal hypofractionated high-dose radiotherapy combined with chemotherapy for non-small-cell lung cancer (NSCLC).

Methods: The combined treatment was carried out in 63 cases of NSCLC and the short-term effects, the patients' survival rate and complications were observed.

Results: Local control of tumor growth was achieved in 79.

[Expression characteristics of transforming growth factor-beta1 in human skin at different development stages of gestation].

Objective: To investigate gene expression of transforming growth factor-beta(1) (TGF-beta(1)) and its two upstream signalling factors (smad2 and smad3) in fetal skin at different gestational ages and postnatal skin and its potential biological significance.

Methods: Fetal skin samples of human embryo were obtained from spontaneous abortions at different gestational ages ranging from 13 to 32 weeks, and also skin collected from patients undergoing plastic surgery. After morphological characteristics of skin at different developmental stages were examined histologically, gene expressions of TGF-beta(1), smad2 and smad3 in skin specimens at different developmental stages were examined with reverse transcription-polymerase chain reaction analysis (RT-PCR).

[An early comprehensive prevention and treatment of sepsis in severely burned patients with delayed fluid resuscitation].

Objective: To study an early comprehensive prevention and treatment of sepsis in severely burned patients with delayed fluid resuscitation.

Methods: From January 1990 to December 2001, 72 cases of patients with delayed fluid resuscitation were admitted to our burn department. Two different periods were divided and analyzed retrospectively.

[Effects of carbachol injection in intestine on plasma levels of inflammatory medium in rats during gut ischemia/reperfusion].

Objective: To investigate the effects of carbachol injection in intestine on plasma levels of tumor necrosis factor-alpha(TNF-alpha), interleukin-10 (IL-10) and cortisol in rats during gut ischemia/reperfusion.

Methods: Wistar rats were anaesthetized with soluble pentobarbitone, and subjected to superior mesenteric artery occlusion (SMAO) for 60 minutes, followed by reperfusion for 60 minutes. Animals were divided into three groups, pretreated group (carbachol injection in intestine at 30 minutes after SMAO; 0.

[Gene expression of angiogenesis-related factors in fetal skin at different developmental stages and childhood skin].

Objective: To explore the change in gene expression of angiogenesis-related factors vascular endothelial growth factor (VEGF), angiopoietin-1 (Ang-1), acid fibroblast growth factor (aFGF) and basic fibroblast growth factor (bFGF), in fetal skin at different developmental stages and children skin and their potential biological significances.

Methods: Fetal skin samples of human embryo were obtained from spontaneous abortion at different gestational ages ranging from 13 to 32 weeks, and children skin specimens were collected from child patients (4-12 years) undergoing plastic surgery. After morphological characteristics of skin at different developmental stages were defined histologically gene expressions of VEGF, Ang-1, aFGF and bFGF were examined with reverse transcription-polymerase chain reaction analysis (RT-PCR).

[Experimental study on composite transplantation of acellular porcine dermal matrix and micro-autograft to repair deep burn wound].

Objective: To observe the effect of composite skin graft consisting of heterogeneous (swine) acellular dermal matrix and combined micro-autograft and allograft with the aim at overcoming shortcomings of repairing materials for burn wound.

Methods: Forty-eight Sprague-Dawley rats with full-thickness skin defect on the back were grafted with the composite skin (compound skin grafting group, G) and autologous particulate skin (control group, C), respectively. The area of wound healing and the rates of wound contraction were measured.

[Effects of fibroblast growth factor-10 on the secretions of transforming growth factor-alpha, platelet-derived growth factor-AB and vascular endothelial growth factor by normal adult human keratinocytes in culture].

Objective: To evaluate the effect of fibroblast growth factor-10 (FGF-10) on the secretion of transforming growth factor-alpha (TGF-alpha), platelet-derived growth factor-AB (PDGF-AB) and vascular endothelial growth factor (VEGF) by human keratinocytes.

Methods: Concentrations of FGF-10 used were 4, 16, 125 and 500 ng/ml. Serum-free keratinocyte growth medium without EGF or with EGF were as negative control and positive control, respectively.

[Carbachol alleviates inflammation in gut tissue during ischemia/reperfusion in rats].

Objective: To investigate the effects of carbachol on local inflammation in gut tissue during ischemia/reperfusion (I/R).

Methods: A jejunal sac was formed in Wistar rats. The superior mesenteric artery was occluded (SMAO) for 60 minutes followed by reperfusion for another 60 minutes.

[Biologic significance of neurofilament protein expression in scar tissue].

Objective: To investigate the situation of neurofilament protein (NFP) and epidermal growth factor receptor (EGFR) staining in scars and normal human skin, and attempt to explain the etiology of scarring formation.

Methods: The samples of scar were obtained from post-burn (11-26 months) patients undergoing plastic operation in our burn unit recently, and the samples of control came from skin donor site of the same patient correspondingly. Immunohistochemistry staining technique was employed to examine the protein expression in scars and skin.

[Expression of mRNA and protein of ubiquitin in diaphragmatic muscle in burned rats with sepsis in early stage].

Objective: To study the expressions of mRNA and protein of ubiquitin in diaphragmatic muscle in burned rats with sepsis.

Methods: The model of burn sepsis was replicated by subjecting the rats to 30 percent total body surface area (TBSA) III degree burn followed immediately by administration of endotoxin (6 mg/kg) into the peritoneal cavity. The rats were randomly divided into 2-and 6-hour groups and a normal control group, with 9 rats in each group.