Novel sensitive immunosensor for the selective detection of Engrailed 2 urinary prostate cancer biomarker.

Engrailed 2 (EN2) is a homeodomain-containing transcription factor expressed in prostate cancer (PCa) cell lines and is secreted into the urines. It is nowadays considered as a promising non-invasive biomarker for PCa early diagnosis. Herein, we report the design of an electrochemical immunosensor for EN2 detection.

ePHex: a phase 3, double-blind, placebo-controlled, randomized study to evaluate long-term efficacy and safety of Oxalobacter formigenes in patients with primary hyperoxaluria.

Background: Primary hyperoxalurias (PHs) are rare genetic diseases that increase the endogenous level of oxalate, a waste metabolite excreted predominantly by the kidneys and also the gut. Treatments aim to improve oxalate excretion, or reduce oxalate generation, to prevent kidney function deterioration. Oxalobacter formigenes is an oxalate metabolizing bacterium.

[Adenomatoid odontogenic tumor: about two cases].

Adenomatoid odontogenic tumor is a benign epithelial tumor which mainly affects young women. It usually occurs in the anterior portion of the upper jaw. Diagnosis can be clinically suspected in patients with cyst formation associated with retained tooth but histopathological confirmation is required.

Computational approach and electrochemical measurements for protein detection with MIP-based sensor.

Rapid and accurate detection of proteins in biological fluids is increasingly required in the biomedical environment. Actually, it is performed with conventional techniques, which are generally run by robotized platforms at centralized laboratories. In this work, molecular dynamics calculations and an experimental procedure were conducted to set up electrochemical sensors based on polypyrrol (PPy) molecular imprinted polymers (MIP) for proteins detection.

Spread of -Producing Enterobacteriaceae and OXA-23-Producing Acinetobacter baumannii Sequence Type 2 in Tunisian Seafood.

Bivalves are filter-feeding animals and markers of bacterial pollution. We report a massive spread of through dominant and lineages and/or plasmid subtypes (F31:A4:B1) as well as the presence of OXA-23-producing sequence type 2 (ST2) in seafood, highlighting a direct risk for the consumer. These findings should urge authorities to consider hospital effluents, and also farm and urban effluents, as important sources of extended-spectrum-beta-lactamase (ESBL)/carbapenemase producers that filter-feeding animals can concentrate and further spread to humans.

[Bacteriology of acute exacerbations of chronic obstructive pulmonary disease in Tunisia].

Introduction: The role of bacteria, including atypical organisms, in acute exacerbations of chronic obstructive pulmonary disease (AECOPD) has been assessed in various ways in Tunisia.

Methods: This was a descriptive and analytical study of patients with a mean age of 68.3±10.

Ultrasensitive Electrochemical Sensors for PSA Detection: Related Surface Functionalization Strategies.

Prostate cancer is the most common male cancer in the world. The diagnosis, staging, prognosis and monitoring are usually done with Prostate Specific Antigen (PSA). Biosensors are emerging as a novel analytical technology for PSA detection.

Outbreak of colistin-resistant carbapenemase-producing Klebsiella pneumoniae in Tunisia.

Objectives: Mechanisms of colistin and carbapenem resistance among a collection of Klebsiella pneumoniae isolates recovered in a university hospital in Tunisia were studied.

Methods: In vitro antimicrobial susceptibility testing, S1 nuclease pulsed-field gel electrophoresis (S1-PFGE), Southern blotting and PCR-based replicon typing (PBRT) were performed. Extended-spectrum β-lactamases (ESBLs), carbapenemases, AmpC-type enzymes and mgrB genes were detected by PCR and sequencing.

Extended-spectrum β-lactamases and plasmid-mediated quinolone resistance in enterobacterial clinical isolates from neonates in Tunisia.

This study was conducted to investigate extended-spectrum-β-lactamase (ESBL) producing Enterobacteriaceae isolates from the Center of Maternity and Neonatology of Monastir, Tunisia. Fourty-six strains out of 283 were found to produce ESBL: Klebsiella pneumoniae (n = 37), Escherichia coli (n = 6), Enterobacter cloacae (n = 2), and Citrobacter freundi (n = 1). Genotyping analysis, using ERIC2 and RAPD, showed that strains were clonally unrelated.

[Perceptions of Tunisian women based on the health beliefs model and their practices related to osteoporosis].

Introduction: The aim of this study is to describe beliefs and practices of women related to health and osteoporosis in order to develop effective and targeted interventions for the prevention of this disease in the Tunisian context.

Methods: A descriptive cross-sectional study was conducted among 100 Tunisian women, aged 45 and older, who consult the basic health center in the suburban area of Sousse region (Tunisia). The collection of information was carried out with "osteoporosis health belief scale "developed by Kim and his colleagues validated and translated into Arabic in Tunisia and the questionnaire of "daily calcium inputs calculation" was developed by Patrice Fardellone.

Characterizing the Retinal Function of Psammomys obesus: A Diurnal Rodent Model to Study Human Retinal Function.

Purpose: To compare the retinal function of a diurnal murid rodent, Psammomys obesus, with that of Wistar albino rat and human subjects.

Materials And Methods: Adult Psammomys obesus were captured and transferred to the animal facilities where they were maintained at 25°C with standard light/dark cycles and natural halophilic plants, rich in water and mineral salts. Standard full-field photopic and scotopic electroretinograms were obtained.

[Bowel-associated dermatosis-arthritis syndrome during ulcerative colitis: A rare extra-intestinal sign of inflammatory bowel disease].

Introduction: Bowel-associated dermatosis-arthritis syndrome (BADAS) is characterized by combined pustular skin eruption and arthralgia. It may be associated with inflammatory bowel disease or bowel bypass surgery. We report a case of BADAS in a patient with ulcerative colitis.

Emergence of ST147 Klebsiella pneumoniae Producing OXA-204 Carbapenemase in a University Hospital, Tunisia.

Molecular features of the first carbapenem-resistant Klebsiella pneumoniae isolates (KP1 and KP2) from the University Hospital Tahar Sfar, Tunisia, were investigated. Antimicrobial susceptibility testing, multilocus sequence typing, S1 nuclease pulsed-field gel electrophoresis, Southern blot, and polymerase chain reaction (PCR)-based replicon typing were performed. Extended-spectrum β-lactamases and carbapenemase genes were detected by PCR and sequencing.

Dissemination of multidrug-resistant blaCTX-M-15/IncFIIk plasmids in Klebsiella pneumoniae isolates from hospital- and community-acquired human infections in Tunisia.

This study investigated the molecular features of extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae from hospital- and community-acquired (HA/CA) infections in the region of Mahdia, Tunisia. Among 336 K. pneumoniae isolates recovered from both clinical contexts between July 2009 and December 2011, 49 and 15 were ESBL producers and originated from clinical and community sources, respectively.

Cerebral abscess potentially of odontogenic origin.

Odontogenic origins are rarely implicated in the formation of brain abscesses. The relative paucity of this kind of infection and the difficulty in matching the causative microorganisms of a brain abscess to an odontogenic source can explain the late management of patients. We herein describe a case of a 46-year-old man with a cerebellar abscess that was probably due to an odontogenic infection.

[Treatment of recurrent temporomandibular dislocation by displacement of the zygomatic arch: a modification of Gosserez and Dautrey's technique].

Introduction: Many methods have been reported for the surgical treatment of recurrent anterior temporomandibular dislocation. Most of these techniques are designed to limit the forward migration of the condylar head. The great number of surgical techniques experimented illustrates the difficulty to find a satisfactory method for the treatment of these recurrent dislocations.

[Association between bacterial resistance and antimicrobial consumption].

Unlabelled: The evolution of multidrug resistance remains an alarming topic due to selection pressure related to the inappropriate use of antibiotics.

Objectives: Our work is in this perspective and focuses on the evolution of the consumption of antibiotics active on gram-negative bacilli, and the evolution of bacterial resistance.

Materials And Methods: International indicator of antibiotic consumption was based on the method of Defined Daily Dose reported to the number of days of hospitalization.

[Influence of diabetes mellitus on the prognosis of non-ST-elevation acute coronary syndromes].

Aim Of Study: Analyze the influence of diabetes mellitus on the prognosis, at short and middle term of patients with non-ST-segment-elevation acute coronary syndrome.

Patients And Method: We conducted a retrospective review of 308 consecutive medical records of patients admitted to the care unit of our department with a non-ST-segment-elevation acute coronary syndrome. One hundred and fifty-six patients were diabetics (group 1) and 152 were not (group 2).

[Correlation of TIMI risk score with angiographic extent and severity of coronary artery disease in non-ST-elevation acute coronary syndromes].

Aim Of Study: To determine whether the TIMI risk score correlates with the angiographic extent and severity of coronary artery disease in patients with non-ST-elevation acute coronary syndrome undergoing cardiac catheterization.

Patients And Method: We conducted a retrospective review of 239 medical records of patients who underwent coronary angiography secondary to non-ST-elevation acute coronary syndrome between 2002 and 2006. Patients were classified into three groups according to TIMI risk score: TIMI scores 0 to 2 (group 1: n=121), 3 to 4 (group 2: n=100), and 5 to 7 (group 3: n=18).

Gene frequencies of the HPA-1 to -6 and -15 human platelet antigens in Tunisian blood donors.

Gene frequencies of mainly human platelet antigens (HPA) -1 to -6 and -15 were determined in 116 Tunisian blood donors. The distribution of HPA-1, -3 and -5 systems approach those found in other Maghrebian populations. Tunisians have the highest frequency of HPA-1b and -5b alleles.