372 results match your criteria: "groupement hospitalier nord[Affiliation]"

Evaluation of a New Tandem Mass Spectrometry Method for Sickle Cell Disease Newborn Screening.

Int J Neonatal Screen

November 2024

Laboratoire de Biologie Médicale Multi-Sites (LBMMS), Service de Biochimie et Biologie Moléculaire Grand Est, Hospices Civils de Lyon, 69500 Bron, France.

In France, sickle cell disease newborn screening (SCD NBS) has been targeted to at-risk regions since 1984, but generalization to the whole population will be implemented from November 2024. Although tandem mass spectrometry (MS/MS) is already used for the NBS of several inherited metabolic diseases, its application for SCD NBS has not been widely adopted worldwide. The aim of this study was to evaluate a dedicated MS/MS kit (Targeted MS/MS Hemo, ZenTech, LaCAR Company, Liege, Belgium) for SCD NBS and to compare the results obtained with those from an NBS reference center using matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) and cation-exchange high-performance liquid chromatography (CE-HPLC, Variant NBS, Biorad Laboratories, Inc.

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Background And Objectives: Cefiderocol approved dosages are based on a prolonged infusion (PI) of 3 h that may not be adequate in all settings The objective of this study was to identify alternative cefiderocol dosage regimens based on short infusion (SI) or continuous infusion (CI).

Methods: We performed 1000-patient pharmacokinetic/pharmacodynamic (PK/PD) simulations based on a reference population model. Drug penetration into the epithelial lining fluid (ELF) was considered for pneumonia.

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Drug susceptibility testing of Nocardia spp. using the disk diffusion method.

Ann Clin Microbiol Antimicrob

December 2024

Laboratoire de Biologie médicale de Référence des Nocardioses, Groupement Hospitalier Nord, Institut des Agents Infectieux, Hospices civils de Lyon, Lyon, France.

Background: Drug susceptibility testing (DST) for Nocardia spp. is essential to initiate effective antibiotic therapy. Currently, the only recommended technique is the determination of minimum inhibitory concentrations (MICs) by microdilution.

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[Emergence of antifungal resistance: A creeping environmental threat?].

Ann Pharm Fr

December 2024

Académie nationale de pharmacie, Paris, France; Service de pharmacie, groupement hospitalier Nord, hospices civils de Lyon, Lyon, France; Malaria Research Unit, SMITh, ICBMS UMR 5246, université de Lyon, Lyon, France.

Antifungal resistance in humans is a clinical reality of increasing incidence that raises problems for patient care. In this current event, we discuss the link that can be made between the presence of antifungals in the environment and the development of resistance in humans, as well as the ecotoxicology of antifungals. The presence of antifungals in the environment has a health, but also an ecological impact.

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[Automated dispensing cabinet: An evaluation of professional practices that says much more than what the authors write].

Ann Pharm Fr

October 2024

Service pharmaceutique, hôpital de la Croix-Rousse, groupement hospitalier nord, hospices civils de Lyon, 103, grande rue de la Croix-Rousse, 69317 Lyon cedex, France. Electronic address:

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Isavuconazole off-label use as an empirical treatment of invasive fungal infections.

Ann Pharm Fr

October 2024

Service pharmacie, groupement hospitalier Nord, hospices civils de Lyon, Lyon, France; ISPB, faculté de pharmacie de Lyon, université de Lyon, université de Lyon 1, Lyon, France; UMR CNRS 5558, laboratoire de biométrie et biologie évolutive, université de Lyon, université de Lyon 1, Villeurbanne, France.

Given its good tolerance and broad-spectrum, isavuconazole is increasingly used off-label as an empirical therapy of invasive fungal infections. We retrospectively reviewed isavuconazole empirical treatment during a 12-month period in four hospitals. During isavuconazole treatment (n=27), none of the patients had a mycological evidence for fungal infection, but 19% (5/27) developed liver test abnormalities without leading to isavuconazole discontinuation.

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Introduction: First-line oxygenation strategy in patients with acute hypoxaemic respiratory failure consists in standard oxygen or high-flow nasal oxygen therapy. Clinical practice guidelines suggest the use of high-flow nasal oxygen rather than standard oxygen. However, findings remain contradictory with a low level of certainty.

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Article Synopsis
  • A nationwide study in France assessed the effectiveness of fecal microbiota transplantation (FMT) for treating Clostridioides difficile infection (CDI) across 17 centers from 2018 to 2022.
  • The study found an overall FMT success rate of 84.3% with very few severe adverse events, and it identified factors linked to CDI recurrence such as severe chronic kidney disease and inadequate bowel cleansing.
  • Key factors for improving FMT success included proper manufacturing of the transplant, thorough bowel prep, and individualized delivery methods based on patient needs.
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Optimizing antibiotic prophylaxis in obese patients undergoing surgery: Weight-based dosing on the rocks?

Anaesth Crit Care Pain Med

October 2024

Université de Poitiers, INSERM, PHAR2, CHU de Poitiers Service d'Anesthésie Réanimation Médecine Péri-opératoire, F-86000 Poitiers 86021, France. Electronic address:

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A Machine Learning Algorithm to Predict the Starting Dose of Daptomycin.

Clin Pharmacokinet

August 2024

Service de Pharmacologie, Toxicologie et Pharmacovigilance, CHU Limoges, France.

Background And Objective: The dosage of daptomycin is usually based on body weight. However, it has been shown that this approach yields too high an exposure in obese patients. Pharmacokinetic and pharmacodynamic indexes (PK/PD) have been proposed for daptomycin's antibacterial effect (AUC/CMI >666) and toxicity (C0 > 24.

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Through their footprint throughout their life cycle, from production to use, medicines have a significant impact on the environment. Reducing this impact is rarely considered from the perspective of the choices that healthcare professionals might have to make when prescribing or dispensing medicines. Should we consider environmental impact, alongside effectiveness and tolerance, one of the dimensions of the proper use of medicines? To address this question, the 5th Forum of the Association for the Proper Use of Medicines highlighted the main sources of pharmaceutical pollution: the carbon footprint linked to production, greenhouse gas emissions, the impact of residues on water and waste from packaging.

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Human DNA-dependent protein kinase catalytic subunit deficiency: A comprehensive review and update.

J Allergy Clin Immunol

November 2024

Hospices Civils de Lyon, Lyon, France; Centre de Références Maladies Rares, Rhumatismes inflammatoires et les maladies Auto-Immunes Systémiques rares de l'Enfant (RAISE), Lyon, France; Centre International de Recherche en Infectiologie (CIRI), Institut National de la Santé et de la Recherche Médicale (INSERM) U1111, Centre National de la Recherche Scientifique Unité Mixte de Recherche (UMR) 5308, École Normale Supérieure de Lyon, Université Claude Bernard Lyon, Lyon, France; Department of Pediatrics Nephrology, Rheumatology, and Dermatology, Hôpital Femme-Mère-Enfant, Bron, France. Electronic address:

Article Synopsis
  • DNA-PKcs is crucial for repairing DNA double-strand breaks and is linked to a rare immunodeficiency in humans, with few documented cases compared to the well-studied Scid mouse model.
  • Seven patients with mutations in the PRKDC gene showed severe combined immunodeficiency symptoms, including granulomas and autoimmunity, highlighting a predominantly inflammatory clinical picture.
  • Hematopoietic stem cell transplantation has proven effective for many, leading to meaningful recovery of T- and B-cell functions in the long-term follow-up of most patients.
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Concepts for the Development of Person-Centered, Digitally Enabled, Artificial Intelligence-Assisted ARIA Care Pathways (ARIA 2024).

J Allergy Clin Immunol Pract

October 2024

University Clinic of Respiratory and Allergic Diseases, Pulmonary & Allergy Department, Golnik, Slovenia; Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.

Article Synopsis
  • - The traditional healthcare approach often overlooks patients' personal experiences and strengths, focusing mainly on disease treatment. Person-centered care aims to align medical decisions with individual values and preferences, particularly for those with chronic conditions.
  • - This paper seeks to enhance care for rhinitis and asthma by developing digital care pathways and incorporating real-world evidence to create a more patient-centered approach.
  • - Key components of the review include advancements in mHealth, the integration of artificial intelligence, a novel classification system for airway diseases, and proposals for the ARIA 2024 guidelines, all targeting a sustainable and applicable healthcare model.
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Background: This study aimed to compare the humoral responses to mRNA COVID-19 vaccination in people living with HIV (PWH) and HIV-negative individuals.

Methods: We included PWH with an undetectable viral load under ART and HIV-negative participants from the French nationwide ANRS COV-POPART cohort who had received two doses of vaccine as a primary vaccination. We compared humoral response between controls and PWH, stratified by CD4 cell count (<200/mm and ≥200/mm CD4 cell counts) at 1, 6, and 12 months after primary vaccination.

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Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.

J Allergy Clin Immunol

August 2024

Centre International de Recherche en Infectiologie (CIRI), INSERM U1111, Unité Mixte de Recherche 5308, Centre national de la recherche scientifique, Ecole Normale Supérieure de Lyon, Lyon, France. Electronic address:

Background: The contribution of Staphylococcus aureus to the exacerbation of atopic dermatitis (AD) is widely documented, but its role as a primary trigger of AD skin symptoms remains poorly explored.

Objectives: This study sought to reappraise the main bacterial factors and underlying immune mechanisms by which S aureus triggers AD-like inflammation.

Methods: This study capitalized on a preclinical model, in which different clinical isolates were applied in the absence of any prior experimental skin injury.

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Background: Invasive candidiasis is still recognized as a major cause of morbidity and mortality. To support clinicians in the optimal use of antifungals for the treatment of invasive candidiasis, a computerized decision support system (CDSS) was developed based on institutional guidelines.

Objectives: To evaluate the correlation of this newly developed CDSS with clinical practices, we set-up a retrospective multicentre cohort study with the aim of providing the concordance rate between the CDSS recommendation and the medical prescription (NCT05656157).

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Purpose: Rapid, reliable identification of mycobacteria from positive cultures is essential for patient management, particularly for the differential diagnosis of Mycobacterium tuberculosis complex (MTBC) and nontuberculous mycobacteria (NTM) species. The aim of the present study was to evaluate a new "In-Vitro-Diagnostic"-certified PCR kit, FluoroType®-Mycobacteria VER 1.0 (Hain Lifescience GmbH) for NTM and MTBC identification from cultures.

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The H274Y substitution (N2 numbering) in neuraminidase (NA) N1 confers oseltamivir resistance to A(H1N1) influenza viruses. This resistance has been associated with reduced N1 expression using transfected cells, but the effect of this substitution on the enzymatic properties and on the expression of other group-1-NA subtypes is unknown. The aim of the present study was to evaluate the antiviral resistance, enzymatic properties, and expression of wild-type (WT) and H274Y-substituted NA for each group-1-NA.

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Background: Paediatric sepsis is the leading cause of death in children under 5 years. No studies have evaluated the application of the Surviving Sepsis Campaign 2020 (SSC-2020) guidelines in paediatric emergency departments (PEDs).

Objective: To assess physician adherence to the SSC-2020 fluid resuscitation guidelines in children with suspected septic shock in PEDs.

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The World Health Organization (WHO) recently published a list of fungal priority pathogens, including and . The increased level of resistance of is raising concern, considering the availability of only four classes of medicine. The WHO is seeking novel agent classes with different targets and mechanisms of action.

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[Unfractionned heparin wastage at a French university hospital].

Ann Pharm Fr

May 2024

Service pharmaceutique, hôpital de la Croix-Rousse, groupement hospitalier Nord, hospices civils de Lyon, 103, Grande rue de la Croix-Rousse, 69317 Lyon cedex 04, France. Electronic address:

Objectives: In a context of heparin shortage, we studied the wasted quantities in three intensive care units (ICU) of a university hospital where two electric syringe pump (ESP) heparin protocols coexist (20,000UI/48mL used in the cardiology ICU and 25,000UI/50mL use in the medical and surgical ICUs).

Method: We performed a prospective observational study of patients treated with heparin ESP. We collected the information recorded in the prescription software connected to the ESP (dosage, start time, infusion rate, interruption times, date and time of end of treatment).

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Article Synopsis
  • A systematic review and meta-analysis was conducted to evaluate the efficacy and safety of daptomycin compared to standard glycopeptides for complicated Gram-positive infections, focusing on all-cause mortality and clinical outcomes.
  • The analysis included eight randomized controlled trials with a total of 1,095 patients, mainly with complex skin infections, and found no difference in all-cause mortality or clinical cure rates between daptomycin and control groups.
  • Daptomycin showed a higher microbiological cure rate and a significantly lower risk of severe adverse events compared to glycopeptides, although more high-quality trials are needed to confirm these findings, particularly for conditions like bacteraemia and endocarditis.
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