Engineering a CRISPRoff Platform to Modulate Expression of Myeloid Cell Leukemia (MCL-1) in Committed Oligodendrocyte Neural Precursor Cells.

In vitro differentiation of human pluripotent stem cell (hPSC) model systems has furthered our understanding of human development. Techniques used to elucidate gene function during early development have encountered technical challenges, especially when targeting embryonic lethal genes. The introduction of CRISPRoff by Nuñez and collaborators provides an opportunity to heritably silence genes during long-term differentiation.

Before-and-After Photography on Social Media: The Pursuit of Impossible Beauty.

Plastic surgeons and aesthetic medicine professionals often share "before" and "after" clinical cases on social media, but this practice can lead to unintended consequences, such as dysmorphophobia and unrealistic expectations. These before-and-after images, frequently posted on social media, tend to promote unreachable, sometimes retouched, beauty standards, downplaying the complexities of the surgical process, omitting details about postoperative care, recovery, and potential complications. This can create the illusion that perfection is easily attainable through surgery, fostering unrealistic ideals and negatively impacting viewers' self-image and self-worth.

Mimosa Kinetic Façade: Bio-Inspired Ventilation Leveraging the Mimosa Pudica Mechanism for Enhanced Indoor Air Quality.

In light of pressing global health concerns, the significance of indoor air quality in densely populated structures has been emphasized. This research introduces the Mimosa kinetic façade, an innovative design inspired by the adaptive responsiveness of the Mimosa plant to environmental stimuli. Traditional static architectural façades often hinder natural ventilation, leading to diminished air quality with potential health and cognitive repercussions.

Competing-risks analysis for evaluating the prognosis of patients with microinvasive cutaneous squamous cell carcinoma based on the SEER database.

Background: Utilizing the traditional Cox regression model to identify the factors affecting the risk of mortality due to microinvasive cutaneous squamous cell carcinoma (micSCC) may produce skewed results. Since cause-specific mortality can guide clinical decision-making, this study employed the Fine-Gray model based on the Surveillance, Epidemiology, and End Results (SEER) database to identify significant predictive variables for the risk of micSCC-related mortality.

Methods: This study used the information of patients with micSCC who were listed in the SEER database during 2000-2015.

Lipoaspirate stored at a constant low temperature by electric control suppresses intracellular metabolism and maintains high cell viability.

Background: Cell therapy is a useful treatment method for wide spectrum of diseases which utilizes the immunosuppressive and regenerative abilities of administered cells. It is essential to build a transport system of tissues from which cells are harvested, because various external factors, such as temperature, time, air pressure, and vibration affect the cell functions isolated from body tissues. In particular, temperature is a critical factor which determines the viability of the cells and organs.

Sugarcane Light-Colored Lignin: A Renewable Resource for Sustainable Beauty.

Lignin has emerged as a promising eco-friendly multifunctional ingredient for cosmetic applications, due to its ability to protect against ultraviolet radiation and its antioxidant and antimicrobial properties. However, its typical dark color and low water solubility limit its application in cosmetics. This study presents a simple process for obtaining light-colored lignin (LCLig) from sugarcane bagasse (SCB) alkaline black liquor, involving an oxidation treatment with hydrogen peroxide, followed by precipitation with sulfuric acid.

Doxycycline-inducible Expression of Proteins at Near-endogenous Levels in Mammalian Cells Using the Sleeping Beauty Transposon System.

The function of a protein within a cell critically depends on its interaction with other proteins as well as its subcellular localization. The expression of mutants of a particular protein that have selective perturbation of specific protein interaction motifs is a very useful strategy for resolving a protein's mechanism of action in a cellular process. In addition, expression of fluorescent protein fusions is a key strategy for determining the subcellular localization of a protein.

The animal protein hydrolysate attenuates sarcopenia via the muscle-gut axis in aged mice.

Age-related muscle loss and dysfunction, sarcopenia, is a common condition that results in poor quality of life in the elderly. Protein supplementation is a potential strategy for preventing sarcopenia and increasing muscle synthesis, but the effectiveness of protein type and level in improving sarcopenia is not well understood. In this study, we compared animal protein hydrolysate (APH), which has a high protein digestibility-corrected amino acid score (PDCAAS) and low molecular weight, with casein as a control group to investigate the effects and mechanisms of sarcopenia improvement, with a particular focus on the gut-muscle axis.

Could Social Network Influence the Body Perception and Develop Dysmorphophobia?

The concept of body beauty has evolved significantly over the years, reflecting cultural shifts, societal norms, and changing ideals. Unlike the gradual evolution of body perception seen in the past eras, the advent of social media has accelerated the pace of change dramatically. The virtual realm, while offering platforms for self-expression, has also bred a culture of comparison, unrealistic standards, and heightened self-consciousness.

Study on the Shape Characteristics and the Allometry of Leaves for Greenhouse Management.

orchids are highly economical ornamental potted plants. Controlling their production schedule requires information on the leaf development characteristics of the orchids. leaves affect the plant's photosynthesis, respiration, and transpiration.

Next-Generation Organic Beauty Products Obtained from Algal Secondary Metabolites: A Sustainable Development in Cosmeceutical Industries.

Algae lay over most of the earth's habitats, and it is said that there are more algal cells in water than there are stars in the sky. They are among the wealthiest marine resources that are to be deemed harmless, with hardly any deleterious consequences. Recently, they have received a lot of consideration to be used in cosmeceuticals.

A prognostic nomogram for the cancer-specific survival of white patients with invasive melanoma at BANS sites based on the Surveillance, Epidemiology, and End Results database.

Objective: The purpose of this study was to develop a comprehensive nomogram for the cancer-specific survival (CSS) of white patients with invasive melanoma at back, posterior arm, posterior neck, and posterior scalp (BANS) sites and to determine the validity of the nomogram by comparing it with the conventional American Joint Committee on Cancer (AJCC) staging system.

Methods: This study analyzed the patients with invasive melanoma in the Surveillance, Epidemiology, and End Results (SEER) database. R software was used to randomly divide the patients into training and validation cohorts at a ratio of 7:3.

AAV-mediated delivery of a Sleeping Beauty transposon and an mRNA-encoded transposase for the engineering of therapeutic immune cells.

Engineering cells for adoptive therapy requires overcoming limitations in cell viability and, in the efficiency of transgene delivery, the duration of transgene expression and the stability of genomic integration. Here we report a gene-delivery system consisting of a Sleeping Beauty (SB) transposase encoded into a messenger RNA delivered by an adeno-associated virus (AAV) encoding an SB transposon that includes the desired transgene, for mediating the permanent integration of the transgene. Compared with lentiviral vectors and with the electroporation of plasmids of transposon DNA or minicircle DNA, the gene-delivery system, which we named MAJESTIC (for 'mRNA AAV-SB joint engineering of stable therapeutic immune cells'), offers prolonged transgene expression, as well as higher transgene expression, therapeutic-cell yield and cell viability.

Thunb. Fruit Extract Attenuates Psoriatic Skin Inflammation in Tumor Necrosis Factor--Stimulated Human Keratinocyte HaCaT Cells .

Thunb. fruit (HDF) is traditionally used for treating liver diseases and alcohol poisoning. The purpose of this study was to explore the effects of HDF on hyperproliferation, levels of inflammatory cytokines, and signaling mechanisms in human psoriatic keratinocyte HaCaT cells.

Profiling of Secondary Metabolites of Optimized Ripe Ajwa Date Pulp ( L.) Using Response Surface Methodology and Artificial Neural Network.

The date palm ( L.) is a popular edible fruit consumed all over the world and thought to cure several chronic diseases and afflictions. The profiling of the secondary metabolites of optimized ripe Ajwa date pulp (RADP) extracts is scarce.

Recovery from Bell's palsy after treatment using uncultured umbilical cord-derived mesenchymal stem cells: A case report.

Background: Bell's palsy is an idiopathic facial palsy with an unknown cause, and 75% of patients heal spontaneously. However, the other 25% of patients continue experiencing mild or severe disabilities, resulting in a reduced quality of life. Currently, various treatment methods have been developed to treat this disease.

The genetic basis of the leafy seadragon's unique camouflage morphology and avenues for its efficient conservation derived from habitat modeling.

The leafy seadragon certainly is among evolution's most "beautiful and wonderful" species aptly named for its extraordinary camouflage mimicking its coastal seaweed habitat. However, limited information is known about the genetic basis of its phenotypes and conspicuous camouflage. Here, we revealed genomic signatures of rapid evolution and positive selection in core genes related to its camouflage, which allowed us to predict population dynamics for this species.

Tyrosinase inhibitory activity of Sargassum fusiforme and characterisation of bioactive compounds.

Introduction: Sargassum fusiforme (Harvey) Setchell, also known as Tot (in Korean) and Hijiki (in Japanese), is widely consumed in Korea, Japan, and China due to its health promoting properties. However, the bioactive component behind the biological activity is still unknown.

Objectives: We aimed to optimise the extraction conditions for achieving maximum tyrosinase inhibition activity by using two sophisticated statistical tools, that is, response surface methodology (RSM) and artificial neural network (ANN).

Identification and quantification of photodegradation products of disposed expanded polystyrene buoy used in aquaculture.

This study investigated the chemicals extracted from an EPS buoy used in aquaculture, which were subsequently collected from a recycling center. It was observed that the chemicals generated upon photodegradation make disposed buoys more toxic. Analysis of the extracted chemicals revealed the presence of 37 compounds, with four compounds quantitatively determined.

The histologic reaction and permanence of hyaluronic acid gel, calcium hydroxylapatite microspheres, and extracellular matrix bio gel.

Background: The filling materials on the beauty market can be classified into three types: natural biological materials, synthetic polymer materials, and composites containing bioactive substances. However, comparative experimental data is lacking to compare their biological responses and permanence.

Aims: The main object of this study was to evaluate the biological response of these three types of fillers to provide a theoretical basis for clinical application.

Photoprotective Effect of Fermented and Aged Mountain-Cultivated Ginseng Sprout () on Ultraviolet Radiation-Induced Skin Aging in a Hairless Mouse Model.

Interest in foods that promote inner beauty increases with increases in exposure to ultraviolet (UV) rays and with improvements in quality of life. This study was performed to evaluate the efficacy of fermented and aged mountain-cultivated ginseng sprouts (FAMCGSs), which have higher anti-inflammatory and antioxidant effects compared to mountain-cultivated ginseng sprouts (MCGSs), as an inner beauty enhancing food. The effect of orally administered FAMCGSs on UV type B (UVB) radiation-induced skin aging was investigated in a hairless mouse model through analyzing skin parameters including epidermal thickness, transepidermal water loss (TEWL), roughness, moisture, elasticity, and collagen contents.

Prolonged activity of the transposase helper may raise safety concerns during DNA transposon-based gene therapy.

DNA transposon-based gene delivery vectors represent a promising new branch of randomly integrating vector development for gene therapy. For the side-by-side evaluation of the c and systems-the only DNA transposons currently employed in clinical trials-during therapeutic intervention, we treated the mouse model of tyrosinemia type I with liver-targeted gene delivery using both transposon vectors. For genome-wide mapping of transposon insertion sites we developed a new next-generation sequencing procedure called streptavidin-based enrichment sequencing, which allowed us to identify approximately one million integration sites for both systems.

Biotechnological interventions and production of galanthamine in Crinum spp.

Genus Crinum L. is a member of the Amaryllidaceae family having beautiful, huge, ornamental plants with umbels of lily-like blooms that are found in tropical and subtropical climates all over the world. For thousands of years, Crinum has been used as a traditional medicine to treat illnesses and disorders.

Preparation of Multicolor Biomass Carbon Dots Based on Solvent Control and Their Application in Cr(VI) Detection and Advanced Anti-Counterfeiting.

Multicolor fluorescent carbon dots (CDs) have received widespread attention due to their excellent fluorescence performance and promising prospects in anti-counterfeiting and sensing detection. To date, most of the multicolor CDs synthesized are derived from chemical reagents; however, the overuse of chemical reagents during the synthesis process will pollute the environment and limit their application. Herein, multicolor fluorescent biomass CDs (BCDs) were prepared by a one-pot ecofriendly solvothermal method, with spinach as the raw material based on solvent control.

Antioxidant, Tyrosinase, α-Glucosidase, and Elastase Enzyme Inhibition Activities of Optimized Unripe Ajwa Date Pulp () Extracts by Response Surface Methodology.

The Ajwa date ( L., Arecaceae family) is a popular edible fruit consumed all over the world. The profiling of the polyphenolic compounds of optimized unripe Ajwa date pulp (URADP) extracts is scarce.