5 results match your criteria: "and Tianjin Institute of Industrial Biotechnology[Affiliation]"
Engineered Imine Reductase for Asymmetric Synthesis of Dextromethorphan Key Intermediate.
Org Lett
May 2024
Key Laboratory of Engineering Biology for Low-carbon Manufacturing, National Engineering Research Center of Industrial Enzymes, National Center of Technology Innovation for Synthetic Biology, Tianjin Engineering Research Center of Biocatalytic Technology, and Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
()-1-(4-Methoxybenzyl)-1,2,3,4,5,6,7,8-octahydroisoquinoline (()-1-(4-methoxybenzyl)-OHIQ) is the key intermediate of the nonopioid antitussive dextromethorphan. In this study, ()-IR61-V69Y/P123A/W179G/F182I/L212V (M4) was identified with a 766-fold improvement in catalytic efficiency compared with wide-type IR61 through enzyme engineering. M4 could completely convert 200 mM of 1-(4-methoxybenzyl)-3,4,5,6,7,8-hexahydroisoquinoline into ()-1-(4-methoxybenzyl)-OHIQ in 77% isolated yield, with >99% enantiomeric excess and a high space-time yield of 542 g L day, demonstrating a great potential for the synthesis of dextromethorphan intermediate in industrial applications.
View Article and Find Full Text PDFIdentification and structure-based engineering of a dipeptidase CpPepD from Clostridium perfringens for the synthesis of l-carnosine.
J Biotechnol
June 2024
National Engineering Laboratory for Industrial Enzymes and Tianjin Engineering Research Center of Biocatalytic Technology, Key Laboratory of Engineering Biology for Low-carbon Manufacturing, National Center of Technology Innovation for Synthetic Biology, and Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, PR China. Electronic address:
l-Carnosine (l-Car), an endogenous dipeptide presents in muscle and brain tissues of various vertebrates, has a wide range of application values. The enzymatic preparation of l-Car is a promising synthetic method because it avoids the protection and deprotection steps. In the present study, a dipeptidase gene (CpPepD) from Clostridium perfringens with high l-Car synthetic activity was cloned and characterized.
View Article and Find Full Text PDFChemo-Enzymatic Strategy for the Efficient Synthesis of Steroidal Drugs with 10α-Methyl Group and a Side Chain at C17-Position from Biorenewable Phytosterols.
JACS Au
April 2024
National Engineering Research Center of Industrial Enzymes and Tianjin Engineering Research Center of Biocatalytic Technology, Key Laboratory of Engineering Biology for Low-carbon Manufacturing, National Center of Technology Innovation for Synthetic Biology, and Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Article Synopsis
- This study addresses the synthesis challenges of steroidal pharmaceuticals that require a 10α-methyl group, as natural steroid starting materials typically have a 10β-methyl group that is hard to convert.
- The researchers utilized a compound called HIP-IPA as an effective intermediate for producing steroidal active pharmaceutical ingredients (APIs) with a specific side chain.
- Genetic modifications in strains of ATCC 6841 led to successful transformations of phytosterols, resulting in a 62% yield of a key intermediate for dydrogesterone synthesis, highlighting a new pathway for developing steroid-based medicines using readily available biobased materials.
Uridine attenuates obesity, ameliorates hepatic lipid accumulation and modifies the gut microbiota composition in mice fed with a high-fat diet.
Food Funct
March 2021
School of Food Science and Technology, State Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China.
Uridine (UR) is a pyrimidine nucleoside that plays an important role in regulating glucose and lipid metabolism. The aim of this study was to investigate the effect of UR on obesity, fat accumulation in liver, and gut microbiota composition in high-fat diet (HFD)-fed mice. ICR mice were, respectively, divided into 3 groups for 8 weeks, that is, control (CON, n = 12), high fat diet (HFD, n = 16), and HFD + UR groups (0.
View Article and Find Full Text PDFHigh-throughput screening of microchip-synthesized genes in programmable double-emulsion droplets.
Nanoscale
March 2017
Department of Biomedical Engineering, Duke University, Durham, 27705, USA. and Department of Biomedical Engineering, Columbia University, New York, 10027, USA and Department of Systems Biology, Columbia University, New York, 10027, USA.
The rapid advances in synthetic biology and biotechnology are increasingly demanding high-throughput screening technology, such as screening of the functionalities of synthetic genes for optimization of protein expression. Compartmentalization of single cells in water-in-oil (W/O) emulsion droplets allows screening of a vast number of individualized assays, and recent advances in automated microfluidic devices further help realize the potential of droplet technology for high-throughput screening. However these single-emulsion droplets are incompatible with aqueous phase analysis and the inner droplet environment cannot easily communicate with the external phase.
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