Response surface optimization for recovery of polyphenols and carotenoids from leaves of using an ethanol-based solvent system.

Response surface methodology has been used to optimize the extraction conditions for total phenolics and carotenoids from leaves of . Solvent concentration (30%-100%), extraction temperature (30-60°C), and extraction time (30-90 min) were used as the independent variables. A second-order polynomial model produced a satisfactory fitting of the experimental data with regard to total phenolics (  = 84.