Effect of Moderate Red Meat Intake Compared With Plant-Based Meat Alternative on Psychological Well-Being: A 10-Wk Cluster Randomized Intervention in Healthy Young Adults.

Background: A healthy diet has been proposed to support good mental health, but the addition of either red meat or meat alternatives is nuanced.

Objectives: We aimed to determine if psychological and physiological well-being is differentially affected by consuming recommended weekly amounts of either lean red meat or plant-based meat alternatives (PBMAs) supplemented with a plant-rich diet.

Methods: The trial was a parallel 2-arm randomized intervention of 10 wk duration.

Structures of Polyacetoxybriaranes Extracted from the Sea Whip Octocoral Junceella fragilis.

In the present study, an algae-containing octocoral, Junceella fragilis, was subjected to chemical screening. The analysis resulted in the extraction of six polyacetoxybriaranes: a new compound, identified as fragilide Z (1), alongside previously identified analogs, which included 12-epi-fragilide G (2), fragilide P (3), junceellolide D (4), junceellonoid A (5), and juncin ZI (6). The structures of compounds 2-6 were investigated through single-crystal X-ray diffraction analysis, whereas that of 1 was examined through two-dimensional nuclear magnetic resonance analysis.

Diagnostic Accuracy of Computational Fluid Dynamics-Based Fractional Flow Reserve Derived From Coronary Angiography: The ACCURATE Study.

Background: Although fractional flow reserve (FFR) is the contemporary standard to detect hemodynamically significant coronary stenosis, it remains underused for the need of pressure wire and hyperemic stimulus. Coronary angiography-derived FFR could break through these barriers. The aim of this study was to assess the feasibility and performance of a novel diagnostic modality deriving FFR from invasive coronary angiography (AccuFFRangio) for coronary physiological assessment.

Transcriptome analysis and functional identification of transfer RNA-derived fragments in grape leaves exposed to UV-C radiation.

Transfer RNA-derived fragments (tRFs) are noncoding small RNAs derived from transfer RNAs (tRNAs) in microorganisms, animals and plants. In plants, tRFs are known to respond to environmental stimuli, including heat, oxidative stress and UV radiation; however, their specific functions in horticultural plants, such as grapevine, remain poorly understood. In this study, we used RNA-seq to identify differentially expressed genes (DEGs) in grape leaves exposed to UV-C radiation.

Transcriptional Reprogramming Deploys a Compartmentalized 'Timebomb' in Catharanthus roseus to Fend Off Chewing Herbivores.

The evolutionary arms race between plants and insects has led to key adaptive innovations that drive diversification. Alkaloids are well-documented anti-herbivory compounds in plant chemical defences, but how these specialized metabolites are allocated to cope with both biotic and abiotic stresses concomitantly is largely unknown. To examine how plants prioritize their metabolic resources responding to herbivory and cold, we integrated dietary toxicity bioassay in insects with co-expression analysis, hierarchical clustering, promoter assay, and protein-protein interaction in plants.

Reproductive toxicity and molecular mechanisms of benzo[a]pyrene exposure on ovary, testis, and brood pouch of sex-role-reversed seahorses (Hippocampus erectus).

As a flagship taxa for marine ecosystems, seahorses possess a unique reproductive strategy of "male pregnancy". They are severely threatened by marine petroleum-based pollution but the molecular mechanism involved remains unclear. We evaluated the toxic effects and mechanisms of sub-acute exposure to benzo[a]pyrene (BaP), a representative polycyclic aromatic hydrocarbon (PAH), at three environmental related dosages (0.

High leptin levels in blood are associated with chronic widespread pain in rheumatoid arthritis.

Background: High body mass index (BMI) has been shown to have an association with chronic widespread pain (CWP), both in Rheumatoid arthritis (RA) and in other pain conditions such as fibromyalgia. Research on the adipose tissue and it's adipokines, for example the well described leptin, is emerging. The objective of this study was to determine if there is an association between leptin levels in blood and CWP in patients with RA.

A Lithiophilic Donor-Acceptor Polymer Modified Separator for High-Performance Lithium Metal Batteries.

Lithium-ion batteries are approaching their theoretical limits. To achieve higher energy density, the development of lithium metal batteries (LMBs) is essential. However, uncontrolled ion transport and unstable solid electrolyte interface (SEI) layer are key factors inducing lithium dendrite growth, hindering the development of LMBs.

Cooperative Molecular Interaction-Based Highly Efficient Capturing of Ultrashort- and Short-Chain Emerging Per- and Polyfluoroalkyl Substances Using Multifunctional Nanoadsorbents.

The short-chain (C to C) and ultrashort-chain (C to C) per- and polyfluoroalkyl substances (PFAS) are bioaccumulative, carcinogenic to humans, and harder to remove using current technologies, which are often detected in drinking and environmental water samples. Herein, we report the development of nonafluorobutanesulfonyl (NFBS) and polyethylene-imine (PEI)-conjugated FeO magnetic nanoparticle-based magnetic nanoadsorbents and demonstrated that the novel adsorbent has the capability for highly efficient removal of six different short- and ultrashort-chain PFAS from drinking and environmental water samples. Reported experimental data indicates that by capitalizing the cooperative hydrophobic, fluorophilic, and electrostatic interaction processes, NFBS-PEI-conjugated magnetic nanoadsorbents can remove ∼100% short-chain perfluorobutanesulfonic acid within 30 min from the water sample with a maximum absorption capacity of ∼234 mg g.

Nomograms Predicting Survival, Recurrence and Beneficiary Identification of Adjuvant Chemotherapy in Treatment-naïve Patients with Rectal Cancer who Underwent Upfront Curative Resection: A multi-institutional study.

Objective: To create and validate nomograms predicting overall survival and recurrence in treatment-naïve rectal cancer (RC) patients who underwent upfront surgery.

Background: Although multidisciplinary treatment is standard for locally advanced RC, understanding surgical efficacy is important for determining indications for perioperative adjuvant therapy.

Methods: RC patients who underwent upfront surgery at the Japanese Society for Cancer of the Colon and Rectum institutions were analyzed.

Comparative Efficacy of Neurofeedback Interventions for Attention-Deficit/Hyperactivity Disorder in Children: A Network Meta-Analysis.

Objective: This study aimed to synthesize and encapsulate findings from recent research (May 1, 2018 to August 1, 2023) on neurofeedback interventions for children diagnosed with attention deficit hyperactivity disorder (ADHD).

Methods: A comprehensive search was conducted across major databases and platforms, including randomized controlled trials s focusing on children aged 5-11 years with ADHD. The inclusion was broad, not restricted by ADHD subtype, gender, IQ, socioeconomic status, or coexisting conditions.

Multi-Disciplinary Investigation Identifies Increased Potency of Ethyl-Parathion Inhaled Within a Soil-Dust Matrix to Cause Acetylcholinesterase-Dependent Molecular Impacts.

Neurotoxicity investigations of inhaled organophosphorus pesticide (OP), ethyl-parathion (EP), were conducted in Sprague Dawley rats comparing exposures to EP volatilized at 0, 1, 10, and 20mg/m versus EP incorporated into soil dust (5mg/m) at 0, 0.0095, 0.09, and 0.

Blood flow dynamics in the arterial and venous parts of the capillary.

Although there is currently sufficient information on various parameters of capillary blood flow, including the average values of blood velocity, there is no data on the dynamics of velocity and the mechanisms of its modulation in various parts of the capillary. The main idea of this work is to develop a tool and image data processing to study the characteristics of the capillary blood flow dynamics. In this study, using the developed method of high-speed videocapillaroscopy, the red blood cells (RBC) velocities in the arterial and venous parts of the nailfold capillaries were compared and a time-frequency analysis of the dynamics of the velocity signals with the calculation of phase coherence was performed.

Roles of spermary-specific carboxylesterases in Nilaparvata lugens reproduction: Opposite between insecticide-induced upregulation and resistance-associated overexpression.

Seminal fluid proteins (SFPs) in insect males are critical for reproduction, being transported into female ovary. In Nilaparvata lugens, a significant rice pest, seven spermary-specific carboxylesterases (CarEs) were found abundantly in SFPs, with over-expression in males of an imidacloprid-resistant (RES) strain compared to a susceptible (SUS) strain. This study aimed to evaluate roles of spermary-specific CarEs in N.

Baseline tritium measurements in Thailand's water bodies: Supporting sustainable nuclear energy development.

Tritium, a radioactive isotope produced naturally through cosmic radiation interactions and anthropogenically through nuclear weapons testing, poses potential environmental risks, particularly within the water cycle. This study measured tritium concentrations in surface water across Thailand to establish a baseline dataset for monitoring potential contamination from nuclear activities and accidents. Surface water samples were collected from 14 large reservoirs during the wet season in October 2023 and the dry season in February 2024, providing a total of 28 samples.

Transient receptor potential vanilloid 4 gene-deficiency attenuates the inhibitory effect of 5,6-dihydroxy-8Z,11Z,14Z,17Z-eicosatetraenoic acid on vascular permeability in mice.

We investigated whether an anti-inflammatory lipid metabolite named 5,6-DiHETE reduces vascular permeability by inhibiting TRPV4 channels in vivo. In wild-type (WT) mice, histamine-induced dye extravasation was reduced by pre-administration of 5,6-DiHETE. In TRPV4-deficient mice, extravasation and histamine-induced edema were already reduced, and 5,6-DiHETE had no additional effect.

Phase separation of MYB73 regulates seed oil biosynthesis in Arabidopsis.

MYB family transcription factors (TFs) play crucial roles in plant development, metabolism, and responses to various stresses. However, whether MYB TFs are involved in regulating fatty acid biosynthesis in seeds remains largely elusive. Here, we demonstrated that transgenic Arabidopsis (Arabidopsis thaliana) plants overexpressing MYB73 exhibit altered FATTY ACID ELONGATION1 (FAE1) expression, seed oil content, and seed fatty acid composition.

Enhancing D-lactic acid production from non-detoxified corn stover hydrolysate via innovative F127-IEA hydrogel-mediated immobilization of T15.

Background: The production of D-lactic acid (D-LA) from non-detoxified corn stover hydrolysate is hindered by substrate-mediated inhibition and low cell utilization times. In this study, we developed a novel temperature-sensitive hydrogel, F127-IEA, for efficient D-LA production using a cell-recycle batch fermentation process.

Results: F127-IEA exhibited a porous structure with an average pore size of approximately 1 μm, facilitating the formation of stable clusters within the gel matrix.

Exploring the phylogenetic framework and trait evolution of Impatiens through chloroplast genome analysis.

Background: The genus Impatiens, which includes both annual and perennial herbs, holds considerable ornamental, economic, and medicinal value. However, it posed significant challenges for taxonomic and systematic reconstruction. This was largely attributed to its high intraspecific diversity and low interspecific variation in morphological characteristics.

Disturbing egg yolk protein structure via pH-shifting treatment for interface reorganization: Improving solubility to enhance oil-water interface adsorption and emulsification properties.

This study explored the impact of varying alkalinity levels in pH-shifting treatments on egg yolk protein (EYP) emulsification and investigated the underlying oil-water interface adsorption mechanism. Increasing alkaline pH-shifting treatment exposed more hydrophobic groups within EYP, altering its tertiary structure. Moreover, pH-shifting treatment reduced solution particle size (P < 0.

Estimation of PEMFC optimal parameters based on an improved butterfly optimization algorithm.

This paper introduces a novel butterfly optimization algorithm, called the spiral search and dynamic crossover based butterfly optimization algorithm (SCBO), for parameter estimation in proton exchange membrane fuel cell (PEMFC) models. To enhance the global performance of the butterfly algorithm, a spin-search strategy is incorporated to expand its exploration range, while an adaptive factor is introduced to strike a balance between exploration and exploitation. Additionally, a dynamic crossover operation is integrated to enhance solution diversity, addressing the algorithm's tendency to converge to local optima.

Complete genome sequence of bacteriophage SeKF_80, isolated from wastewater in British Columbia.

is a Gram-negative inhabitant of the gastrointestinal tract of warm-blooded animals and commonly implicated in foodborne illness. Here, we describe the isolation of phage SeKF_80. The 89,965 bp genome contains 174 predicted coding sequences with 44 predicted functions.

Effect of changes in skin properties due to diabetes mellitus on the titration period of transdermal fentanyl: single-center retrospective study and diabetic animal model study.

Background: In the dose titration of transdermal fentanyl to prevent unrelieved pain, it is important to consider not only dose adjustment, but also the titration period, which is influenced by the time required to reach the steady state. Many patients with cancer pain experience comorbidities that might affect the skin properties and influence transdermal absorption. We hypothesized that skin changes due to diabetes mellitus (DM) would affect the titration period of transdermal fentanyl.

USP21-EGFR signaling axis is functionally implicated in metastatic colorectal cancer.

The emerging role of ubiquitin-specific peptidase 21 (USP21) in stabilizing Fra-1 (FOSL1) highlights its involvement in promoting colorectal cancer (CRC) metastasis. Additionally, a reciprocal link between EGFR signaling and Fra-1 activation has been identified, mediated through matrix metalloproteinases (MMPs). However, the functional implications of the USP21-EGFR signaling axis in metastatic CRC (mCRC) are not fully understood.

MiR-378 exaggerates angiogenesis and bone erosion in collagen-induced arthritis mice by regulating endoplasmic reticulum stress.

Rheumatoid arthritis (RA) is a chronic autoimmune disorder marked by pain, inflammation, and discomfort in the synovial joints. It is critical to understand the pathological mechanisms of RA progression. MicroRNA-378 (miR-378) is highly expressed in the synovium of RA patients and positively correlated with disease severity, but its function and underlying mechanisms remain poorly understood.