11 results match your criteria: "a Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences[Affiliation]"
Dokl Biochem Biophys
August 2016
State Research Institute for Genetics and Selection of Industrial Microorganisms, 1 Dorozhnyi pr. 1, Moscow, 117545, Russia.
Electrochemical parameters of bacterial cells Shewanella oneidensis MR-1 were investigated. For registration of the direct electron transfer between S. oneidensis MR-1 and electrode, bacterial cells were pretreated with didodecyldimethylammonium bromide (DDAB), a synthetic membrane-like substance of polycationic nature that exhibits membrane-loosening properties.
View Article and Find Full Text PDFAnal Bioanal Chem
October 2015
Institute for Energy Problems of Chemical Physics of the Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334, Moscow, Russia.
Real-time feedback about dissected tissue during the neurosurgical procedure is strongly requested. A novel direct ionization mass spectrometric method for identifying pathological differences in tissues is proposed. The method is based on simultaneous extraction of tissue lipids and electrospray ionization which allows mass spectrometric data to be obtained directly from soft tissues.
View Article and Find Full Text PDFInt J Nanomedicine
June 2015
Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia.
Atomic force microscopy (AFM) was applied to carry out direct and label-free detection of gp120 human immunodeficiency virus type 1 envelope glycoprotein as a target protein. This approach was based on the AFM fishing of gp120 from the analyte solution using anti-gp120 aptamers immobilized on the AFM chip to count gp120/aptamer complexes that were formed on the chip surface. The comparison of image contrasts of fished gp120 against the background of immobilized aptamers and anti-gp120 antibodies on the AFM images was conducted.
View Article and Find Full Text PDFSAR QSAR Environ Res
February 2015
a Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow , Russia.
Protein-protein interactions (PPI) are involved in most of the essential processes that occur in organisms. In recent years, PPI have become the object of increasing attention in drug discovery, particularly for anti-HIV drugs. Although the use of combinations of existing drugs, termed highly active antiretroviral therapy (HAART), has revolutionized the treatment of HIV/AIDS, problems with these agents, such as the rapid emergence of drug-resistant HIV-1 mutants and serious adverse effects, have highlighted the need for further discovery of new drugs and new targets.
View Article and Find Full Text PDFExpert Rev Proteomics
February 2014
Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, 119121, Pogodinskaya Street 10, Moscow, Russia.
Today, proteomics usually compares clinical samples by use of bottom-up profiling with high resolution mass spectrometry, where all protein products of a single gene are considered as an integral whole. At the same time, proteomics of proteoforms, which considers the variety of protein species, offers the potential to discover valuable biomarkers. Proteoforms are protein species that arise as a consequence of genetic polymorphisms, alternative splicing, post-translational modifications and other less-explored molecular events.
View Article and Find Full Text PDFJ Chem Inf Model
February 2014
Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Building 10/8, Pogodinskaya Str., Moscow, 119121, Russia.
A new ligand-based method for the prediction of sites of metabolism (SOMs) for xenobiotics has been developed on the basis of the LMNA (labeled multilevel neighborhoods of atom) descriptors and the PASS (prediction of activity spectra for substances) algorithm and applied to predict the SOMs of the 1A2, 2C9, 2C19, 2D6, and 3A4 isoforms of cytochrome P450. An average IAP (invariant accuracy of prediction) of SOMs calculated by the leave-one-out cross-validation procedure was 0.89 for the developed method.
View Article and Find Full Text PDFJ Proteome Res
January 2014
Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, 10 Pogodinskaya Street, Moscow 119121, Russia.
We report the results obtained in 2012-2013 by the Russian Consortium for the Chromosome-centric Human Proteome Project (C-HPP). The main scope of this work was the transcriptome profiling of genes on human chromosome 18 (Chr 18), as well as their encoded proteome, from three types of biomaterials: liver tissue, the hepatocellular carcinoma-derived cell line HepG2, and blood plasma. The transcriptome profiling for liver tissue was independently performed using two RNaseq platforms (SOLiD and Illumina) and also by droplet digital PCR (ddPCR) and quantitative RT-PCR.
View Article and Find Full Text PDFBiochim Biophys Acta
January 2014
Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences (RAMS), Russia. Electronic address:
The Human Proteome Project (HPP) was started two years ago and the international consortia have elaborated a number of informational resources to harbor the HPP data. Selected informational resources are currently used to elaborate the HPP baseline metrics, which were introduced to estimate future contribution of HPP to the knowledge domain. We developed a Web-based tool Gene-centric Content Management System (GenoCMS) for comparing public resources to proprietary results by using the representation of proteins as color-coded catalog.
View Article and Find Full Text PDFBioinformatics
August 2013
Laboratory for Structure-Function Based Drug Design, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia.
Summary: Experimentally found gene expression profiles are used to solve different problems in pharmaceutical studies, such as drug repositioning, resistance, toxicity and drug-drug interactions. A special web service, DIGEP-Pred, for prediction of drug-induced changes of gene expression profiles based on structural formulae of chemicals has been developed. Structure-activity relationships for prediction of drug-induced gene expression profiles were determined by Prediction of Activity Spectra for Substances (PASS) software.
View Article and Find Full Text PDFThe final goal of the Russian part of the Chromosome-centric Human Proteome Project (C-HPP) was established as the analysis of the chromosome 18 (Chr 18) protein complement in plasma, liver tissue and HepG2 cells with the sensitivity of 10(-18) M. Using SRM, we have recently targeted 277 Chr 18 proteins in plasma, liver, and HepG2 cells. On the basis of the results of the survey, the SRM assays were drafted for 250 proteins: 41 proteins were found only in the liver tissue, 82 proteins were specifically detected in depleted plasma, and 127 proteins were mapped in both samples.
View Article and Find Full Text PDFJ Chem Inf Comput Sci
April 2003
Laboratory of Structure-Function Based Drug Design, V.N. Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, 10 Pogodinskaya Street, Moscow 119121, Russia.