In-house preparation and evaluation of (125)I-histamine progesterone tracer for radioimmunoassay of progesterone.

The progesterone tracer for the development of in-house radioimmunoassay was prepared in two steps clearly described. First, activation of 11α-hemisuccinate progesterone (progesterone-11 α HS) and labeling of histamine with (125)I, and second, conjugation of activated 11α-HS progesterone with iodinated histamine. The purification of the tracer was carried out by gel filtration on Sephadex G-25.