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A combined treatment using ethylmethane sulfonate and ultraviolet light to compare amylase production by three Bacillus sp. isolates.
Prep Biochem Biotechnol
February 2019
a Istanbul Protein Reserach and Inovation Center (PROMER), Istanbul , Turkey.
Article Synopsis
- Three Bacillus species producing amylase enzymes were isolated from soil and identified using genetic analysis, with Bacillus subtilis showing the highest activity levels.
- Amylase production was measured at around 10 U/mL, with total protein at 15 mg/mL, and the enzyme activity was enhanced by 58% through combined treatments of ethylmethane sulfonate (EMS) and ultraviolet (UV) light.
- The study demonstrates that B. subtilis subtilis can be effectively utilized in industrial applications for producing amylase, highlighting a novel method to increase enzyme activity.