Two-level delivery systems based on CaCO cores for oral administration of therapeutic peptides.

Two-level systems for oral delivery of therapeutic peptides were developed; the carriers consist of CaCO cores included into alginate granules. Such systems were first used for the delivery of low molecular weight drugs. It was shown that efficiency of encapsulation of peptides depends on their pI value, hydrophobicity, characteristics of the compounds used for doping CaCO cores, their surface potential and the techniques employed for loading peptides into the first-level carriers.

Alginate-containing systems for oral delivery of superoxide dismutase. Comparison of various configurations and their properties.

The regularities of release of therapeutic antioxidant enzyme superoxide dismutase (SOD) from various alginate-based delivery systems (DS) into simulated gastric and intestinal fluids were determined. The following systems were used: Ca-alginate granules (AG) prepared by various methods, porous carbonate cores with multilayer polyelectrolyte coating as well as the new two-level DS (Ca-AG containing carbonate cores loaded with proteins). The influence of the method of granule preparation, composition of gelation bath and ionic composition of the simulated fluids on release profiles of the protein from different DS was revealed.