[Relationship of polymorphism of LMP2 gene to insulin-dependent diabetes mellitus and DR3 gene].

Objective: To investigate the relationship of polymorphism of large multifunctional protease (LMP) 2 genes to insulin-dependent diabetes mellitus (IDDM) and DR3 gene.

Methods: The polymorphism of LMP2 genes was identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 68 IDDM patients and 71 healthy controls. IDDM patients and healthy controls were respectively divided into 2 groups according to DR3 genotyping.

[A modified technique for normothermic bloodless hepatic resection. Experimental and clinical studies].

On the basis of experimental study of canine normothermic total hepatic vascular blockade (NTHVB) through portal decompression by means of jugulo-splenic shunting. We proposed a critical period of the hepatic ischemic time with NTHVB be 45 minutes. The conventional technique of Heaney's bloodless hepatic resection was modified, including the cancellation of the synchronous blockade of the abdominal aorta and effective application of limited hepatic ischemic time to the critical manipulation during operation through the combination of various techniques for hepatectomy.