Expert consensus on clinical genome sequencing interpretation and reporting.

Genome sequencing (GS) refers to a technology that comprehensively and systematically detects the DNA sequences of an individual's nuclear and mitochondrial genomes. It aims to identify genetic variants and investigate their roles in human health and disease progression. As an emerging diagnostic tool, GS offers significant support for clinical diagnosis due to its high throughput, accuracy, and comprehensiveness.

Tailoring foaming behavior of poly(lactic acid) by different geometric sizes of bio-based polyamide.

Naturally derived materials have attracted much attention owing to the demand of sustainability. In this work, bio-based polyamide was used to tailor the foaming behavior of poly(lactic acid) by dispersing into different geometric sizes. The results showed that both micro-sized spherical and fibrillar polyamide markedly shortened the half-crystallization time of poly(lactic acid) in isothermal crystallization process, while nanofibrils played a better role on promoting crystallization than submicron spheres.

Postharvest ripening-induced modification of cell wall polysaccharide affects plum phenolic bioavailability.

The mechanisms by which cell wall polysaccharides regulate phenolic release are essential to human health. Scanning electron microscopy (SEM), surface area and porosimetry analyzer, high-performance liquid chromatography (HPLC), and atomic force microscopy (AFM) indicated that compared to fresh plums, postharvest ripening reduced chain linearity in the homogalacturonan region of pectins and the degree of branching of RG-I; pectin and hemicellulose underwent solubilization and depolymerization by cell wall-degrading enzymes; and the specific surface area of cellulose was reduced by 19.5 %-26.

Insight into carbon structural variation from steam gasification of rice straw on enhancing hydrogen generation.

Converting biomass waste into hydrogen energy through gasification is a crucial pathway for producing "green hydrogen". In a fixed bed reactor, a representative biomass waste, rice straw (RS), was pyrolyzed at N, HO, CO, and O atmospheres to generate hydrogen. Solid C-13 Nuclear Magnetic Resonance Spectroscopy (C-NMR) and Fourier Transform infrared spectroscopy (FTIR) were employed to elucidate the carbon structure and functional groups of the samples.

HnRNPM inhibits pseudorabies virus replication by inducing apoptosis in infected cells.

Heterogeneous nuclear ribonucleoproteins (hnRNPs) are a family of RNA-binding proteins that play crucial roles in RNA processing, transcriptional regulation, nucleocytoplasmic transport, and apoptosis. As a member of the hnRNP family, heterogeneous nuclear ribonucleoprotein M (hnRNPM) has been implicated in diverse cellular processes, including the regulation of tumor-associated gene expression, promotion of angiogenesis, enhancement of tumor cell invasion and metastasis, and modulation of RNA virus replication. However, the interaction between hnRNPM and pseudorabies virus (PRV) remains unexplored.

Low salt protein-based emulsion: Health and quality win-win challenge.

Due to the natural salt solubility of proteins as emulsifiers, a large amount of salt is required to fill the emulsion system to facilitate the completion and stability of the emulsion emulsification process. High salt diets and edible products have been rejected and gradually replaced by health products dominated by low salt concepts. However, low salt conditions can make it difficult for emulsion systems to form or collapse.

A programmable multichannel chemiluminescence immunoassay sensor for automatic quantification of the total amount of fumonisin B, B and B.

A programmable multichannel chemiluminescence immunoassay sensor was developed based on the biotin-streptavidin system for the automatic quantification of the total amount of fumonisin B, B and B in maize samples. First, the key parameters, such as the reaction time and reagent dosage were optimized to improve the sensitivity. All reagents were subsequently added to the corresponding wells of the reagent strips, which were further sealed to create a total fumonisin quantification kit.

Exploring metabolite-mediated links between lipidome and deep vein thrombosis: Insights from Mendelian randomization analysis.

The aim was to investigate the causal relationship between the lipidome and deep vein thrombosis (DVT) while identifying and quantifying the role of metabolites as potential mediators. Two-sample Mendelian randomization (MR) analysis of lipid species (n = 7174) and DVT (6767 cases and 330,392 controls) was performed using pooled data from genome-wide association studies. In addition, we quantified the proportion of metabolite-mediated lipidomic effects on DVT using 2-step MR.

Postoperative pneumonia in isolated coronary artery bypass grafting: A comprehensive study of epidemiology, etiology, and disease burden from China (2020-2023).

Objective: To investigate the epidemiological trends,etiological profiles and disease burden metrics related to postoperative pneumonia following isolated coronary artery bypass grafting (CABG).

Methods: A retrospective analysis was conducted on data from 518 patients who developed postoperative pneumonia following isolated CABG between January 1, 2020, and November 30, 2023.

Results: Postoperative pneumonia occurred at a rate of 11.

A fuzzy robust optimization model for dual objective forward and reverse logistics networks considering carbon emissions.

The inherent unpredictability within the low-carbon integrated supply chain logistics network complicates its management. This paper endeavours to address the challenge of designing a low-carbon logistics network within a context of uncertainty and with consideration of low-carbon policies. It also endeavours to identify locations of facilities and appropriate transportation routes between nodes.

The Relationship Between the Heterogeneity of Lumbar Vertebral Trabecular Bone Mineral Density Distribution and Osteoporotic Vertebral Fractures.

This study aims to investigate the relationship between the spatial distribution and heterogeneity of lumbar vertebral trabecular volumetric bone mineral density (vBMD) and osteoporotic vertebral fractures(OVF). This retrospective study included the L1 and L2 vertebrae of 143 participants with osteoporotic vertebral fractures and 429 age- and sex-matched no-fractured controls. Spatial distribution was assessed using the superior/middle and inferior/middle ratios, while heterogeneity was indicated by the quartile coefficient of variation (QCV) and interquartile range (IQR).

Innovative Syringe-Guided Barbed Sutures for Orbital Fat Repositioning: A Retrospective Study.

Objective: To investigate the clinical efficacy of a method that corrects tear trough deformity by subcutaneous fixation of orbital septal fat flaps using syringe-guided absorbable barbed sutures.

Methods: A retrospective analysis was conducted on the clinical data of 125 cases with lower eyelid bags accompanied by tear trough deformity who were treated in the Department of Plastic Surgery at Zhengzhou People's Hospital from 2020 to 2024. Among them, there were 13 males and 112 females, with an average age of 34.

Stimuli-Responsive Circularly Polarized Luminescence of Gold Clusters Based on Hydrogen-Bond Driven Intercluster Coupling.

Stimuli-responsive circularly polarized luminescence (CPL) metal clusters hold significant potential in high-security encryption and sensing applications, yet the exploration of hydrogen-bond-driven CPL-active metal clusters remains limited. Here, we report the synthesis of an enantiomeric pair of rhomboid Au4 clusters utilizing chiral R/S-4-hydroxymethyl-5-methyloxazole-2-thione (R/S-HMMT) ligands. Two enantiomeric pairs of self-assembled metal clusters R/S-Au4(HMMT)4-blue and R/S-Au4(HMMT)4-red were obtained, by constructing distinct intercluster hydrogen bonds through the use of different crystalline solvents.

Stacking Pressure Modulated Deposition and Dissolution of Zinc Anode.

Aqueous zinc-ion batteries (ZIBs) are emerging as a promising candidate for large-scale energy storage, offering enhanced safety and low costs. Nevertheless, the disordered growth of zinc dendrites has resulted in low coulombic efficiency and the dangers of short circuits, limiting the commercialization of ZIBs. In this study, a planar growth of zinc along the (002) direction is achieved by regulating the moderate initial stacking pressure during cell cycling and facilitating a larger zinc deposition particle size.

Unraveling Compressive Strain and Oxygen Vacancy Effect of Iridium Oxide for Proton-Exchange Membrane Water Electrolyzers.

Iridium-based electrocatalysts are commonly regarded as the sole stable operating acidic oxygen evolution reaction (OER) catalysts in proton-exchange membrane water electrolysis (PEMWE), but the linear scaling relationship (LSR) of multiple reaction intermediates binding inhibits the enhancement of its activity. Herein, the compressive strain and oxygen vacancy effect exists in iridium dioxide (IrO)-based catalyst by a doping engineering strategy for efficient acidic OER activity. In situ synchrotron characterizations elucidate that compressive strain can enhance Ir─O covalency and reduce the Ir─Ir bond distance, and oxygen vacancy (O) as an electronic regulator causes rapid adsorption of water molecules on the Ir and adjacent Ov (Ir─O) pair site to be coupled directly into O─O intermediates.

Exploring Carbon Dots for Biological Lasers.

Biological lasers, representing innovative miniaturized laser technology, hold immense potential in the fields of biological imaging, detection, sensing, and medical treatment. However, the reported gain media for biological lasers encounter several challenges complex preparation procedures, high cost, toxicity concerns, limited biocompatibility, and stability issues along with poor processability and tunability. These drawbacks have impeded the sustainable development of biological lasers.

The relationship between negative emotions and adjustment disorder in young adults: the mediating role of rumination and insomnia.

Introduction: This study aimed to investigate the influence of negative emotions on adjustment disorder (AjD) in young adults, focusing on the mediating role of rumination and insomnia.

Methods: The study recruited 2015 young patients (aged 18-35) receiving treatment at the Psychosomatic Medicine Department of the Affiliated Encephalopathy Hospital of Zhengzhou University from February 2023 to March 2024. Participants completed the Depression Anxiety and Stress Scale (DASS), Ruminative Responses Scale (RRS), Insomnia Severity Scale (ISI), and Adjustment Disorder - New Module 20(ADNM-20) to assess negative emotions, rumination thinking, sleep status, and AjD.

Advances in targeting protein S-palmitoylation in tumor immunity and therapy.

S-palmitoylation is a reversible and dynamic post-translational modification of proteins. A palmitoyl group is covalently attached to a cysteine residue of the protein by a thioester link. It regulates the transcription and expression of downstream target genes and cell signaling, influencing cellular functions.

[The regulation and mechanism of apolipoprotein A5 on myocardial lipid deposition].

The current study aimed to clarify the roles of apolipoprotein A5 (ApoA5) and milk fat globule-epidermal growth factor 8 (Mfge8) in regulating myocardial lipid deposition and the regulatory relationship between them. The serum levels of ApoA5 and Mfge8 in obese and healthy people were compared, and the obesity mouse model induced by the high-fat diet (HFD) was established. In addition, primary cardiomyocytes were purified and identified from the hearts of suckling mice.

The DNA N6-adenine methylation target gene GhMAF1 promotes fiber initiation at the base of Gossypium ovules.

DNA methylation consists of 5-methylcytosine and N6-methyl deoxyadenosine (6mA) and is crucial in plant development. However, its specific role and potential mechanism to initiate cotton fibers remain unclear. This study employed Oxford Nanopore Technologies (ONT) sequencing to analyze DNA methylation alterations in ZM24 and ZM24 fuzzless-lintless (ZM24fl) during fiber initiation.

Engineered extracellular vesicles promote the repair of acute kidney injury by modulating regulatory T cells and the immune microenvironment.

Background: Acute kidney injury (AKI) is a common and severe clinical condition. However, the underlying mechanisms of AKI have not been fully elucidated, and effective treatment options remain limited. Studies have shown that immune cells play a critical role in AKI, with regulatory T cells (Tregs) being one of the most important immunosuppressive lymphocytes.

Development and preliminary validation of the evaluation scale for nurses' core emergency response abilities in sudden major infectious disease outbreaks.

Background: In recent years, the escalating frequency and intensity of infectious disease outbreaks have underscored the profound severity and global ramifications of public health challenges confronting healthcare systems. As frontline responders during emerging infectious disease crises, nurses' core emergency response competencies critically influence the timeliness and efficacy of outbreak prevention and control measures. Currently, standardized assessment instruments for evaluating nurses' core emergency response capabilities during major infectious disease outbreaks remain underdeveloped.

Study on the dynamic prediction and optimum regulation scheme of water resource carrying capacity in the yellow river basin.

The dynamic change in regional water resource carrying capacity (WRCC) is an important factor in formulating an optimal water resource allocation scheme. To accurately predict the dynamic change in WRCC and formulate the optimal regulation scheme in a river basin, this paper proposes a diagnostic index system of WRCC based on the mutual feeding mechanism of economic-society, water resources and eco-environment. The multi-methods including support vector machine (SV) and system dynamics (SD), back propagation neural network (BP), etc.

Transcriptome combined with single-cell sequencing explored prognostic markers associated with T cell exhaustion characteristics in head and neck squamous carcinoma.

Head and neck squamous cell carcinoma (HNSC) ranks among the most prevalent cancers worldwide, characterized by significant heterogeneity and a complex immune microenvironment. T cell exhaustion is pivotal in the pathogenesis of HNSC, where depleted T cells exhibit reduced proliferative capacity and diminished effector function, facilitating tumor immune escape and subsequent disease progression. A thorough understanding of the primary mechanisms driving T cell depletion within the tumor microenvironment is essential for enhancing the efficacy of immunotherapeutic approaches in HNSC, with profound implications for patient outcomes.