14 results match your criteria: "Zhejiang Universitygrid.13402.34 School of Medicine[Affiliation]"

Two-Color CRISPR Imaging Reveals Dynamics of Herpes Simplex Virus 1 Replication Compartments and Virus-Host Interactions.

J Virol

December 2022

Department of Cell Biology and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang Universitygrid.13402.34 School of Medicine, Hangzhou, China.

Real-time imaging tools for single-virus tracking provide spatially resolved, quantitative measurements of viral replication and virus-host interactions. However, efficiently labeling both parental and progeny viruses in living host cells remains challenging. Here, we developed a novel strategy using the CRISPR-Tag system to detect herpes simplex virus 1 (HSV-1) DNA in host cells.

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Cefiderocol is a novel siderophore cephalosporin that displays activity against Gram-negative bacteria. To establish cefiderocol susceptibility levels of Acinetobacter baumannii strains from China, we performed susceptibility testing and genomic analyses on 131 clinical isolates. Cefiderocol shows high activity against the strains.

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Treatment of osteomyelitis is still challenging, as conventional antibiotic therapy is limited by the emergence of resistant strains and the formation of biofilms. Sonoantimicrobial chemotherapy (SACT) is a novel therapy of low-frequency and low-intensity ultrasound (LFLIU) combined with a sonosensitizer. Therefore, in our study, a sonosensitizer named emodin (EM) was proposed to be combined with LFLIU to relieve acute osteomyelitis caused by methicillin-resistant Staphylococcus aureus (MRSA) through antibacterial and antibiofilm effects.

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Here, a program was designed to surveil the colonization and associated infection of OXA-232-producing carbapenem-resistant Klebsiella pneumoniae (CRKP) (OXA-232-CRKP) in an intensive care unit (ICU) and to describe the epidemiological characteristics during surveillance. Samples were sourced from patient and environment colonization sites in the ICU from August to December 2019. During the surveillance, 106 OXA-232-CRKP strains were isolated from 8,656 samples of colonization sites, with an average positive rate of 1.

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Longitudinal 16S rRNA Sequencing Reveals Relationships among Alterations of Gut Microbiota and Nonalcoholic Fatty Liver Disease Progression in Mice.

Microbiol Spectr

June 2022

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang Universitygrid.13402.34 School of Medicine, Hangzhou, China.

Nonalcoholic fatty liver disease (NAFLD) is a prevalent and progressive disease spectrum ranging from nonalcoholic fatty liver (NAFL) to nonalcoholic steatohepatitis (NASH), yet there is no effective treatment and efficient noninvasive diagnostic method for NASH. The present study investigated the longitudinal alternations of gut microbiota in the Western diet (WD) induced murine NAFLD model using 16S rRNA sequencing. Evident steatosis and inflammation were detected in the liver at the 8th and 12th week, while prompted hepatic oxidative injury and fibrosis were found at the 16th week.

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Emergence of Colistin Resistance Gene - in Isolates Recovered from Fecal Samples of Chickens, Slaughterhouse Workers, and a Nearby Resident.

Microbiol Spectr

April 2022

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital of Medical School, College of Medicine, Zhejiang Universitygrid.13402.34, Hangzhou, China.

The wide spread of plasmid-borne mobilized colistin resistance () genes from animals to humans broadly challenges the clinical use of polymyxins. Here, we evaluated the incidence of a recently reported variant, -, in animals and humans in the same area. Our results revealed the presence of novel -carrying plasmids in two Klebsiella pneumoniae isolates from chickens, one Escherichia coli isolate from slaughterhouse workers, and a chromosome-borne gene in Enterobacter kobei from a healthy resident in the same region.

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Neuronal miR-138 Represses HSV-2 Lytic Infection by Regulating Viral and Host Genes with Mechanistic Differences from HSV-1.

J Virol

May 2022

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang Universitygrid.13402.34 School of Medicine, Hangzhou, Zhejiang, China.

Herpes simplex virus 2 (HSV-2) establishes latent infection in dorsal root ganglion (DRG) neurons after productive (lytic) infection in peripheral tissues. A neuron-specific microRNA, miR-138, favors HSV-1 latency by repressing viral and host and genes, yet the role of miR-138 in HSV-2 infection was unknown. The ICP0 mRNAs of HSV-1, HSV-2, and chimpanzee herpesvirus each have one to two canonical miR-138 binding sites.

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Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) is a threat to global public health. We characterized a sequence type 17 (ST17) K. pneumoniae clinical isolate that was resistant to carbapenems and belonged to serotype KL38/O2.

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Article Synopsis
  • Carbapenem-resistant Pseudomonas aeruginosa (CRPA) is increasingly problematic in hospitals, particularly in China, with a rise in strains that are resistant to the antibiotic ceftazidime-avibactam (CZA).
  • A novel variant of the KPC gene, named KPC-90, was identified as the cause of CZA resistance in a specific CRPA strain, indicating a genetic change due to drug exposure.
  • The combination of the KPC-90 mutation and the overexpression of an efflux pump in the resistant strain suggests a significant evolution in antibiotic resistance mechanisms, highlighting the need for better monitoring to control the spread of resistance.
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Dose-Dependent Relationship between Protection of Thioacetamide-Induced Acute Liver Injury and Hyperammonemia and Concentration of Lactobacillus salivarius Li01 in Mice.

Microbiol Spectr

December 2021

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang Universitygrid.13402.34 School of Medicine, Hangzhou, China.

Recently, probiotics have been widely used as an adjuvant therapy to cure, prevent, or improve certain diseases. However, no research has been carried out into the dose of probiotics, especially the maximum dose. Therefore, the effective and safe dosage of probiotics needs to be studied.

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Here, we characterized the mechanisms resulting in the development of KPC-71-mediated resistance to ceftazidime-avibactam (CZA) during treatment of carbapenem-resistant Klebsiella pneumoniae (CRKP) infections. CZA-susceptible and CZA-resistant K. pneumoniae strains, namely, KP357 and KP697, were isolated from the same patient.

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Article Synopsis
  • - The study analyzed 374 carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates from seven hospitals in China, discovering that 40.4% produced Klebsiella pneumoniae carbapenemase (KPC), with around 50.3% of KPC-PA isolates resistant to ceftazidime-avibactam (CAZ-AVI).
  • - Researchers identified various plasmid types associated with KPC-PA and found that mobile genetic elements were crucial for their transmission; additionally, plasmid curing restored susceptibility to CAZ-AVI in some strains.
  • - The findings indicate that KPC-PA poses a growing clinical threat, highlighting the need for cautious use of CA
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The emergence of daptomycin-resistant (DAP-R) Staphylococcus aureus strains has become a global problem. Point mutations in are the main cause of daptomycin (DAP) treatment failure. However, the impact of these specific point mutations in methicillin-resistant S.

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The rapid spread of the gene is a major public health concern. Here, we describe the multidrug-resistant Proteus mirabilis strain XH1653, which contains a novel SXT/R391 integrative and conjugative element (ICE), harboring two tandem copies of and 21 other resistance genes. XH1653 was resistant to all antibiotics tested, apart from aztreonam.

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