[Mechanisms of pethidine-induced vasodilatation].

Objective: To investigate the vasodilating effects of pethidine, particularly in association with intracellular calcium.

Methods: Aorta rings of Sprague-Dawley rats, with or without endothelium, were prepared in organ bath to measure the vascular tone. Pre-contractions by KCl (80 mmol/L) and phenylephrine (PhE) (10(-6)mol/L) were induced.

[Improvement of cardiomyocyte isolation for different purposes].

Objective: To improve the methods of isolating single ventricular myocytes for purposes of different experiments.

Methods: The cardiac ventricular myocytes were isolated enzymatically by Langendorff perfusion technique at constant flow rate. The patch clamp whole-cell recording, a spectrofluorometric method and a video tracking system were used to verify the basic electrophysiological properties, intracellular calcium transient and cell contraction of the single myocytes.

[Lipid-peroxidantion damage of embryo and placenta induced by artesunate in rats].

Objective: To examine the effect and mechanism of artesunate (Art) on embryo development.

Methods: Rat embryo and placental glutathione peroxidase (GSH-Px)and malondialdehyde (MDA) were identified by using DTNB (dithionitrobenzene) direct method and TBA (thiobarbituric acid). We investigated the damage of decidual cells caused by Art using cell culture techniques.

[Preparation of monoclonal antibodies against oh(8)dG and their biological characteristics].

Objective: To prepare monoclonal antibodies against oh(8)dG and to evaluate the relationship between Hp infection and oxidative DNA damage by detecting oh8dG in gastric mucosa.

Methods: BALB/C mice were immunized with BSA-oh(8)dG conjugate, monoclonal antibodies were prepared by hybridoma technique, the biological characteristics of antibodies were analysed by competitive ELISA, Western blot and immunohistochemistry.

Results: Two strains of hybridoma cell were obtained.