Identification and validation of transcriptome-wide association study-derived genes as potential druggable targets for osteoarthritis.

Aims: Osteoarthritis (OA) is a widespread chronic degenerative joint disease with an increasing global impact. The pathogenesis of OA involves complex interactions between genetic and environmental factors. Despite this, the specific genetic mechanisms underlying OA remain only partially understood, hindering the development of targeted therapeutic strategies.

Risk Prediction Models for Stress Urinary Incontinence in Pregnant and Postpartum Women: A Systematic Review and Critical Appraisal.

Background: Many studies have developed or validated prediction models to estimate the risk of stress urinary incontinence (SUI) in pregnant and postpartum women, but the quality of the model development and model applicability remains unknown.

Objectives: To systematically review and critically evaluate currently available prediction models for SUI in pregnant and postpartum women.

Search Strategy: Cochrane Library, EBSCO, PubMed, Web of Science, EMBASE, Chinese CNKI, Wanfang and VIP databases were searched from inception until February 2024.

Treat-And-Extend Versus Pro Re Nata Regimen of Intravitreal Conbercept Injection for Neovascular Age-Related Macular Degeneration: Results from COCOA, a Prospective, Open-Label, Multicenter, Randomized Phase IV Clinical Trial.

Purpose: To evaluate and contrast the effectiveness and safety of two conbercept treatment protocols-a three-dose treat-and-extend (3+T&E) regimen and a three-dose pro re nata (3+PRN) regimen-in Chinese patients diagnosed with neovascular age-related macular degeneration (nAMD).

Methods: Eligible patients, who had not undergone anti-VEGF intraocular injections within 3 months prior to enrollment, were randomly assigned to either the 3+T&E or 3+PRN regimen. The 3+T&E group received at least three monthly injections, with subsequent visit intervals extended based on disease activity assessment.

Tailoring OER/ORR activity in TMN catalysts through first-/second-shell nitrogen doping: a density functional theory investigation.

Proposing an effective modification strategy to optimize catalyst reaction potentials is crucial for enhancing catalytic performance. In this study, we employed a combined approach by adjusting nitrogen dopants in the first- and second-shell environments to tailor the OER/ORR reaction potentials of FeN, CoN, and NiN active centers. Using density functional theory simulations, we systematically compared the effects of first- and second-shell nitrogen dopants on the local atomic/electronic structures and catalytic activities.

Loss of N-6 adenine-specific DNA methyltransferase 1 leads to meiotic prophase abnormalities and male sub-fertility in mice.

Mammalian sexual reproduction critically relies on the generation of haploid gametes following a specialized cell division process known as meiosis. Here, we demonstrate that N-6 Adenine-Specific DNA methyltransferase 1 (N6AMT1) plays a crucial role in the progression of meiosis during spermatogenesis, as follows. N6AMT1 was expressed in germ cells throughout the entire process of spermatogenesis, with a peak in mRNA levels in spermatocytes at the prophase I stage of meiosis.

Cascade-Responsive Nanoprodrug Disrupts Immune-Fibroblast Communications for Potentiated Cancer Mechanoimmunotherapy.

The abnormal tumor mechanical microenvironment due to specific cancer-associated fibroblasts (CAFs) subset and low tumor immunogenicity caused by inefficient conversion of active chemotherapeutic agents are two key obstacles that impede patients with desmoplastic tumors from achieving stable and complete immune responses. Herein, it is demonstrated that FAP-αCAFs-induced stromal stiffness accelerated tumor progression by precluding cytotoxic T lymphocytes. Subsequently, a cascade-responsive nanoprodrug capable of re-educating FAP-αCAFs and amplifying tumor immunogenicity for potentiated cancer mechanoimmunotherapy is ingeniously designed.

Heteroatom Introduction and Electrochemical Reconstruction on Heterostructured Co-Based Electrocatalysts for Hydrogenation of Quinolines.

Electrocatalytic hydrogenation (ECH) of quinoline provides an eco-friendly and prospective route to achieve the highly value-added generation of 1,2,3,4-tetrahydroquinoline (THQ). Co element has been proven to be the efficient catalytic site for ECH of quinoline, but the rational regulation of the electronic structure of active Co site to improve the activity is still a challenge. Herein, the hierarchical core-shell structure consisting of NiCo-MOF nanosheets encapsulated Cu(OH) nanorods (Cu(OH)@CoNi-MOF) is constructed.

Flexible Patterned Fuel Cell Patches Stimulate Nerve and Myocardium Restoration.

The distribution of electrical potentials and current in exogenous electrostimulation has significant impacts on its effectiveness in promoting tissue repair. However, there is still a lack of a flexible, implantable power source capable of generating customizable patterned electric fields for in situ electrostimulation(electrical stimulation). Herein, this study reports a fuel cell patch (FCP) that can provide in situ electrostimulation and a hypoxic microenvironment to promote tissue repair synergistically.

Impregnating Lattice Sulfur into Iron Crystal Allows Anaerobic Degradation of Extracellular Antibiotic Resistance Genes.

Lattice sulfur-impregnated nanoscale zerovalent iron (S-nFe) has been recognized as a promising groundwater remediation agent. However, little information is available on its reactivity with ubiquitous extracellular antibiotic resistance genes (eARGs) in anaerobic groundwater, and how S content and speciation affect their interactions. Here, the efficient anaerobic degradation of eARGs by S-nFe (6 log within 5 min), resulting in completely inhibited transformation is showed.

Testing syndemic models along pathways to psychotic spectrum disorder: implications for population-level preventive interventions.

Background: Population-level preventive interventions are urgently needed and may be effective for psychosis due to social determinants. We tested three syndemic models along pathways from childhood adversity (CA) to psychotic spectrum disorder (PSD) and their implications for prevention.

Methods: Cross-sectional data from 7461 British men surveyed in 5 population subgroups.

Injectable Nanocomposite Hydrogels for Intervertebral Disc Degeneration: Combating Oxidative Stress, Mitochondrial Dysfunction, and Ferroptosis.

Intervertebral disc degeneration (IVDD) is a major cause of low back pain, where oxidative stress and mitochondrial dysfunction are key contributors. Additionally, ferroptosis, an iron-dependent form of cell death, is identified as a critical mechanism in IVDD pathogenesis. Herein, the therapeutic potential of gallic acid (GA)-derived PGA-Cu nanoparticles, enhanced with functional octapeptide (Cys-Lys-His-Gly-d-Arg-d-Tyr-Lys-Phe, SS08) to build the mitochondria-targeted nanoparticles (PGA-Cu@SS08), and embedded within a hydrogel matrix to form a nanocomposite hydrogel, is explored.

spores displaying GRA12 induce immunity against acute toxoplasmosis.

Background: () is a widely prevalent intracellular parasite that infects almost all warm-blooded animals and causes serious public health problems. The drugs currently used to treat toxoplasmosis have the disadvantage of being toxic and prone to the development of resistance, and the only licensed vaccine entails a risk of virulence restoration. The development of a safe and effective vaccine against is urgently needed.

TRP channels and breast cancer: the role of TRPs in the pathophysiological development.

TRP channels play important roles in regulating various physiological and pathological processes, including the progression of cancer. Several TRP channels mediate tumour development. This review focuses on the role of TRP channels in the development of breast cancer, including their involvement in proliferation, apoptosis, autophagy, metastasis, and angiogenesis.

Evaluating human exposure to antibiotic resistance genes.

Antibiotic resistance is an escalating global concern, leading to millions of annual fatalities. Antibiotic resistance genes (ARGs) present in bacteria equip them to withstand the effects of antibiotics. Intra- and interspecific ARGs transmission through horizontal gene transfer further exacerbates resistance dissemination.

Circulating short-chain and branched short-chain fatty acids and the risk of incident type 2 diabetes: findings from the 4C study.

Previous studies suggested that fecal short-chain fatty acids (SCFAs) and branched short-chain fatty acids (BCFAs) are associated with glucose regulation. However, the potential relationship between circulating SCFAs and BCFAs with incident diabetes risk in both men and women remains unidentified in prospective cohort studies. In this study, we examined a panel of nine serum SCFAs and BCFAs in 3414 subjects with incident diabetes, and matched normoglycemic controls from the China Cardiometabolic Disease and Cancer Cohort study.

Synergistic antifungal effects and mechanisms of amantadine hydrochloride combined with azole antifungal drugs on drug-resistant .

Introduction: The increasing resistance of () to conventional antifungal drugs poses a great challenge to the clinical treatment of infections caused by this yeast. Drug combinations are a potential therapeutic approach to overcome the drug- resistance of . This study explored the synergistic effects of amantadine hydrochloride (AMH) combined with azole antifungal drugs against drug-resistant and .

Study on the impact of rural public sports facilities and instructors on residents' participation in sports activities in China.

Objective: This study aims to explore how rural public sports facilities and their instructors influence the participation of rural residents in sports activities under the background of China's rural revitalization strategy. The goal is to provide strategies for the effective use and management of rural sports facilities, thereby encouraging rural residents to actively participate in sports activities, improve their quality of life, and support comprehensive rural revitalization.

Methods: A cross-sectional study design was used, employing a stratified sampling method to distribute questionnaires to 5,000 residents in the eastern, central, western, and southern regions of China.

Daily physical activity, coffee and energy drink consumption, and sleep patterns among Chinese elite professional esports athletes: a case study of Zhejiang Regans Gaming.

Purpose: Esports is increasingly recognized as both a legitimate sport and profession. However, evidence on the health behaviors of esports athletes remains limited. Due to the nature of their activity, these athletes face occupational health risks associated with prolonged sedentary behavior, sleep deprivation, and excessive energy drink consumption.

Parkin characteristics and blood biomarkers of Parkinson's disease in WPBLC study.

Background: The exact mechanisms of PD are unclear, but Parkin-mediated mitophagy dysfunction is believed to play a key role. We investigated whether blood levels of Parkin and other biomarkers are linked to the risk of developing PD.

Methods: Baseline blood measures of Parkin and other biomarkers, including Homocysteine, carcinoembryonic antigen, Urea, total proteins, total cholesterol, creatine kinase, and albumin, were collected from 197 clinically diagnosed Parkinson's disease participants and 107 age-matched healthy controls in Wenzhou Parkinson's Biomarkers and Living Characteristics study.

Genomic identification of a pair of multidrug-resistant but non-pathogenic serovar Goldcoast isolates in southeast China.

Introduction: is an important foodborne pathogen that can induce severe diseases such as gastrointestinal disease and typhoid fever. Accumulating evidence revealed that 's resistance to antibiotics also seriously affects human health. Pathogenic serovar Goldcoast ( Goldcoast) was first detected in 2010 in China and was predicted to have an increasing tendency.

High prevalence of carbapenem-resistant and identification of a novel VIM-type metallo-β-lactamase, VIM-92, in clinical isolates from northern China.

Carbapenem-resistant (CRPA) has become a serious global health concern due to the limited treatment options. The primary resistance mechanism in CRPA involves the production of metallo-β-lactamases (MBLs), making MBL-producing a significant component of CRPA cases. To understand the prevalence of CRPA in hospitals in northern China, we conducted a preliminary screening and identification of CRPA in 143 clinical isolates of collected from various departments of a tertiary hospital between 2021 and 2023, analyzing CRPA resistance trends in certain regions of northern China during this period.

Corrigendum: Inhibition effect of AGEs formation by the two novel peptides EDYGA and DLLCIC derived from .

[This corrects the article DOI: 10.3389/fnut.2025.

mutations in with negative MPT64 antigen assay results from a tertiary hospital in Southeastern China.

Background: MPT64 protein is an effective marker for detecting (MTB) in liquid culture and clinical tissue samples. However, some MTB clinical isolates test negative for this antigen because of varied mutation types across different regions.

Methods: DNA samples of MPT64 antigen assay-negative MTB strains were collected from a tertiary hospital from January 2016 to January 2024, and gene mutations were detected by sequencing.

Citrate as a safe and effective alternative to heparin for catheter locking: a systematic review and meta-analysis of randomized controlled trials.

Background: Consensus on the use of citrate vs.heparin for catheter locking remains elusive, with ongoing controversy. This meta-analysis investigates the efficacy and safety of citrate lock solutions compared to heparin lock solutions in preventing catheter-related complications.

Dose-dependent Relationship between Alcohol Consumption and the Risks of Hepatitis B Virus-associated Cirrhosis and Hepatocellular Carcinoma: A Meta-analysis and Systematic Review.

Background And Aims: The quantitative effects of alcohol consumption on cirrhosis and hepatocellular carcinoma (HCC) in hepatitis B virus (HBV) infection are unknown. This study aimed to establish a dose-dependent model of alcohol consumption on the risks of cirrhosis and HCC.

Methods: PubMed, Embase, the Cochrane Library, Web of Science, and four Chinese databases were searched for studies published from their inception to 15 May 2024.