Construction of an Efficient Engineered Strain for Chaetoglobosin A Bioresource Production from Potato Starch Industrial Waste.

Chaetoglobosin A (CheA), a typical structure of the cytochalasin family, exhibits outstanding efficacy against a variety of tumor cells and plant pathogens. However, its low yield and high production cost are major obstacles limiting its wide application. In order to increase CheA yield, an engineered strain was established by overexpressing , the gene encoding the MFS family's efflux pump, on chassis cells lacking , which have been shown to act as a negative regulator of CheA biosynthesis.

The Overall Quality Changes of Chinese Sauced Ducks at Different Stages During Processing and Storage.

This study reveals the physicochemical, microbial, flavor, and sensory changes in sauced duck from the marinating phase to the end of storage, divided into six stages (stages A-F). The changes in color, total plate count, total volatile basic nitrogen, and thiobarbituric acid reactive substance at different stages were clarified. We utilized 16S rRNA gene sequencing, GC-IMS, and GC-MS to explore the changes in bacterial flora, fatty acid composition, and flavor characteristics.

β-Ionone Treatment Enhances the Antioxidant Capacity in Postharvest Broccoli ( L. var. ) by Maintaining the Levels of Bioactive Substances.

Broccoli is prone to nutrient loss during postharvest storage due to its high respiratory metabolism. In this study, we investigated the effects of 0.1 mm β-ionone on bioactive substances and antioxidant capacity during postharvest storage of broccoli.

Overview of Glycometabolism of Lactic Acid Bacteria During Freeze-Drying: Changes, Influencing Factors, and Application Strategies.

Lactic acid bacteria (LAB) play a vital role in food fermentation and probiotics microeconomics. Freeze-drying (FD) is a commonly used method for preserving LAB powder to extend its shelf life. However, FD induces thermal, osmotic, and mechanical stresses that can impact the glycometabolism of LAB, which is the process of converting carbohydrates into energy.

The Effects of Nisin Treatment on the Phenylpropanoid and Physiological Mechanisms of Fresh-Cut Pumpkin.

Pumpkin is rich in nutritional value, and it can be eaten as a vegetable or as a staple food, making it popular among modern consumers. However, after fresh cutting, pumpkins are susceptible to moisture loss, softening, microbial contamination, and browning, all of which significantly compromise their quality during storage. Therefore, it is essential to develop effective preservation techniques for maintaining the quality of fresh-cut pumpkins.

Effects of Cold Post-Fermentation Process on Microbial Diversity and Biogenic Amines in Protease-Assisted Fermented .

This study investigated the effects of enzyme-assisted low-temperature cold fermentation on 's microbial diversity, biogenic amine (BA) formation, and physicochemical properties. The results showed that the enzyme-assisted fermentations for both room- and low-temperature groups (RTEF30 and LTEF20, respectively) significantly increased total acid (TA), amino nitrogen (NH3-N), and enzyme activity compared to the non-enzyme fermentation at room-temperature post-fermentation (RTNF30). This indicated that enzyme-assisted fermentation effectively overcame challenges associated with low-temperature fermentation of .

The Effect of Roasting on Oil Content, Fatty Acids, Bioactive Compounds and Mineral Contents of Purslane ( L.) Seeds.

In this study, the effect of oven and microwave roasting at different times on oil content, total phenol, flavonoid, fatty acids, phenolic components and mineral contents of purslane seeds was investigated. The total phenolic quantities of the purslane seeds roasted in the oven and microwave were characterized to be between 252.0 ± 1.

Preparation of Temperature Resistant Terpolymer Fracturing Fluid Thickener and Its Working Mechanism Study via Simulation Methods.

To enhance oil and gas recovery, a novel hydrophobic terpolymer was synthesized via free radical polymerization. The terpolymer consists of acrylamide, acrylic acid, and hydrophobic monomers, and is used as a hydraulic fracturing fluid thickener for freshwater environments. Hydrophobic groups were introduced into terpolymer to improve its tackiness and temperature resistance.

Developing Eco-Friendly, High-Performance Soy Protein Plywood Adhesive via Core-Shell Hybridization and Borate Chemistry.

Developing eco-friendly, high-performance adhesives is crucial for sustainable industrial applications but remains a significant challenge. Herein, a synergistic strategy combining core-shell hybridization and borate chemistry was employed to fabricate a multifunctional soy protein (SPI) adhesive with excellent adhesion. Specifically, a reactive core-shell hybrid (POSS-U) was synthesized via free-radical polymerization using octavinyl-POSS as the core and urushiol (U) as the shell.

Static Behavior of Post-Installed High-Strength Large-Bolt Shear Connector with Fabricated Hybrid Fiber-Reinforced Concrete/Ordinary Concrete Deck.

Recent research indicates that high-strength bolts could be more effectively and efficiently used to connect steel girders and fabricated decks or retrofit existing composite girders than headed studs. To reduce the number of bolt shear connectors and, thus, further accelerate the construction of composite girders, high-strength large bolts could be an excellent alternative, resulting in greater concrete stress below the bolt. Also, hybrid fiber-reinforced concrete (HFRC) has better tensile ductility and strength than that of ordinary concrete (OC).

Microscopic Mechanical Properties and Physicochemical Changes of Cement Paste Exposed to Elevated Temperatures and Subsequent Rehydration.

The effect of elevated temperatures and subsequent rehydration on the microscopic mechanical properties and physicochemical changes of cement pastes was investigated. Cement pastes with different grades (CEM I 42.5, CEM I 52.

Strain Dependent Evolution of Microstructure and Texture During Cold Rolling of Ferritic Stainless Steel: Experiments and Visco-Plastic Self-Consistent Modeling.

In the present work, the microstructure and texture evolution of ferritic stainless steel during unidirectional cold rolling were investigated, and the Visco-Plastic Self-Consistent (VPSC) polycrystal model was used for the simulation of texture during cold rolling. Comparison of different interaction models was made to obtain a model that better reproduces the texture evolution of ferritic stainless steels. The as-received hot-rolled samples were unidirectionally cold rolled in a laboratory rolling mill, and the thickness was reduced by 30%, 60% and 80%.

Research on the Improvement of High-Temperature Performance of Asphalt by Waste Scallop Shell Powder.

In order to utilize a large amount of waste scallop shells in road engineering and develop environmentally friendly and high-performance asphalt, the impact of scallop shell powder (SSP) on the high temperature performance of base asphalt and styrene-butadiene-styrene (SBS)-modified asphalt was evaluated in this study. The effects of SSP on the conventional properties of base asphalt and SBS-modified asphalt were investigated according to penetration, softening point, ductility, and rotational viscosity at 135 °C, and recommended dosing amounts were given, respectively. The high-temperature rheological properties of SSP-modified asphalt were evaluated according to temperature sweep and multiple stress creep and recovery (MSCR) tests.

Microstructural Evolution and Mechanical Performance of A500 Bulletproof Steel Joints Welded with Austenitic and Ferritic Filler Materials.

This study examines the microstructural evolution and mechanical properties of A500 bulletproof steel joints welded with austenitic stainless steel (ER371) and ferritic (T91) filler materials. While austenitic fillers are traditionally used in bulletproof steel welding to prevent cracking and hydrogen embrittlement, their lower hardness creates a potential weakness in welded joints. This research explores an alternative approach using a newly developed ferritic filler material to achieve strength matching with the base material.

The Relationship Between Social Anxiety and Depression Among Rural High School Adolescents: The Mediating Role of Social Comparison and Social Support.

: This study aimed to explore the impact of social anxiety on depression among high school students and examine the parallel mediating roles of social comparison and social support. : A total of 806 rural high school students were surveyed using the Short Mood and Feelings Questionnaire, the Social Phobia Inventory, the Chinese version of the Social Comparison Orientation Scale, and the Multidimensional Scale of Perceived Social Support. : Social anxiety and social comparison were significantly and positively correlated with depression, while social support exhibited a significant negative correlation with depression.

Kinsenoside Suppresses DGAT1-Mediated Lipid Droplet Formation to Trigger Ferroptosis in Triple-Negative Breast Cancer.

Triple-negative breast cancer (TNBC) presents limited therapeutic options and is characterized by a poor prognosis. Although Kinsenoside (KIN) possesses a wide range of pharmacological activities, its effect and mechanism in TNBC remain unclear. The objective of this research was to explore the therapeutic effectiveness and the molecular mechanisms of KIN on TNBC.

Development of a Pre-Modification Strategy to Overcome Restriction-Modification Barriers and Enhance Genetic Engineering in for Nisin Biosynthesis.

Due to the barriers imposed by the restriction-modification (RM) system, Nisin-producing industrial strains of often encounter low transformation efficiency, which seriously hinders the widespread application of genetic engineering in non-model . Herein, we present a novel pre-modification strategy (PMS) coupled with optimized plasmid delivery systems designed to systematically evade RM barriers and substantially improve Nisin biosynthesis in . Through the use of engineered strains with methylation profiles specifically optimized for C20, we have effectively evaded RM barriers, thereby facilitating the efficient introduction of large Nisin biosynthetic gene clusters into .

Functional Characterization of , , and in Cucumber Defense Against .

Vascular tissue development plays a pivotal role in plant growth and defense against biotic stress. Root-knot nematodes, particularly (), are globally distributed phytopathogens that cause severe economic losses in a variety of vascular plants. In this study, three vascular bundle development-related genes, including , , and , were identified in cucumber.

Comprehensive Analysis of the Synergistic Effects of Bimetallic Oxides in CoM/γ-AlO (M = Cu, Fe, or Ni) Catalysts for Enhancing Toluene Combustion Efficiency.

Catalytic combustion is an efficient and economic technology for eliminating volatile organic compounds (VOCs) in industrial environments. This study evaluated the synergistic catalytic properties of bimetallic oxides, viz., CoM/γ-AlO (M = Cu, Fe, or Ni), for improving the combustion efficiency of toluene.

Determination of Urolithin A in Health Products by Ultra-High-Performance Liquid Chromatography.

This study establishes and validates a novel ultra-high-performance liquid chromatography (UHPLC) method for the determination of urolithin A content in health products, a bioactive compound with potential anti-aging properties. Given the lack of standardized analytical methods for urolithin A in health products, this research addresses a critical gap in quality control. The method employs a methanol-water mobile phase, optimized gradient elution, and a specialized UPLC column (ACQUITY UPLC CSH Fluoro Phenyl) to achieve high resolution and specificity in the separation of urolithin A from its impurities.

Structural Characterization of Polysaccharides from Noni ( L.) Juice and Their Preventive Effect on Oxidative Stress Activity.

Polysaccharides are very promising molecules in the field of pharmacotherapy. Knowing this, the aim of this study was to extract, characterize, and evaluate the action of the polysaccharides in noni juice, using biological models of Type 2 diabetes mellitus processes. In this study, one polysaccharide named NJSPd-1 was separated from fermented noni fruit juice.

2D Porous TiC MXene as Anode Material for Sodium-Ion Batteries with Excellent Reaction Kinetics.

Sodium-ion batteries (SIBs) are a promising electrochemical energy storage system but face great challenges in developing fast-charging anodes. MXene-based composites are a new class of two-dimensional materials that are expected to be widely used in SIB energy storage due to their excellent electrical conductivity and stable structure. However, MXenes tend to experience interlayer stacking during preparation, which can result in poor electrochemical performance and a lower actual capacity compared to the theoretical value.

A Comprehensive Review of the Phytochemistry, Pharmacology and Other Applications of Euphorbiae Humifusae Herba.

Euphorbiae Humifusae Herba (EHH) is a globally distributed plant commonly utilized in traditional Chinese medicine (TCM) and health food within China. The dried aerial parts of EHH are well-recognized as health foods for the management of diarrhea and other intestinal diseases. Known for its therapeutic effects, such as heat-clearing, detoxification, blood cooling, hemostasis, dampness, elimination, and jaundice relief, EHH has yielded 197 bioactive compounds identified to date, including terpenoids, flavonoids, phenolic acids, tannins, alkaloids, sterols, lactones, coumarins, and other constituents, with flavonoids and terpenoids, highlighting its wide-ranging pharmacological properties and medicinal significance.

Versatile Tunable Terahertz Absorption Device Based on Bulk Dirac Semimetals and Graphene.

We employed the CST Microwave Studio software 2020 and the FDID algorithm for simulation. We have designed a terahertz broadband absorber based on Dirac semimetals and graphene, achieving continuous broadband absorption with a rate exceeding 80% over the range from 7.6776 to 9.

Per- and Polyfluoroalkyl Substances (PFAS) in Consumer Products: An Overview of the Occurrence, Migration, and Exposure Assessment.

Per- and polyfluoroalkyl substances (PFASs) have been widely used in the production of consumer products globally due to the excellent water and oil resistance and anti-fouling properties. The multiple toxic effects of some PFASs also pose a threat to human health and ecosystem, and the frequent use of certain consumer products increased the risk of human exposure to PFASs. More data on the occurrence, concentration, and migration of PFASs in consumer products is urgently needed to address the possible risks posed by exposure to consumer products.