A CRISPR/Cas12a-based direct transverse relaxation time biosensor via hydrogel sol-gel transition for Salmonella detection.

This research developed a magnetic relaxation switching (MRS) biosensor based on hydrogel sol-gel transition and the CRISPR/Cas12a system (MRS-CRISPR) to detect Salmonella. Herein, the alkaline phosphatase (ALP) labeled with streptavidin was captured by the biotin-modified DNA on magnetic nanoparticles (MNPs) surface, which generated an acidic environment via enzymatic reaction to release Ca and induced the transformation of alginate sol to hydrogels. In contrast, Salmonella activated the trans-cleavage activity of the CRISPR/Cas12a system, interrupting the capture of ALP and the subsequent sol-gel transition.

Acinetobacter indicus Coharboring Tet(X6) and bla Isolated From Slaughterhouse Waste.

Objectives: Acinetobacter indicus is an important pathogen of nosocomial infection. The purpose of this study was to analyze the resistance and transmission of A. indicus strain AIBD14 isolated from slaughterhouse environment.

Molecular cloning and functional characterization of Caspase-8 in goldfish (Carassius auratus L.).

Apoptosis, a form of programmed cell death, is essential for maintaining homeostasis within the internal environment. Caspase-8, an initiator caspase, plays a pivotal role in activating the caspase cascade during the apoptotic process. This study cloned and expressed Caspase-8 from goldfish, aimed to investigate the role of Caspase-8 in the immune response of fish to bacterial infections, specifically those caused by Aeromonas hydrophila.

Gonadal transcriptome analysis of Opsariichthys bidens reveals sex-associated genes.

Opsariichthys bidens is a unique economically important freshwater fish in China. Male O. bidens grow faster than females, and male fish have attractive blue-green stripes on the body surface during the breeding period.

Extraction, purification, structural identification, and anti-senescent activity of novel pearl peptides on human dermal fibroblasts.

The purpose of the present study was to prepare novel anti-senescent peptides from pearls, characterize their primary sequence and secondary structure, and investigate their protective effects and molecular mechanisms towards D-galactose (D-gal)-induced senescence on human dermal fibroblasts (HDFs). Novel pearl peptides with a purity of 96.58 % and maximum yield of 3.

A comprehensive understanding of the influence and molecular mechanism of exeA on the pathogenicity in Aeromonas hydrophila.

Aeromonas hydrophila is a serious human and animal co-pathogenic bacterium. The type II secretion system (T2SS), a key virulence factor, is vital for the secretion of exotoxins from the bacterium. exeA gene is important for the assembly of the T2SS.

Effects of Different Levels of Antarctic Krill Oil on the Ovarian Development of .

Antarctic krill oil has been proven to be able to promote the ovarian development of crustaceans, but its optimal application dose and potential regulatory mechanism in are still unclear. In this study, five isonitrogenous and isolipidic diets with gradient additions of Antarctic krill oil (0%, 1.5%, 3%, 4.

Functional compounds of Torreya grandis nuts and their processing byproducts: Extraction process, health benefits, and food applications - A comprehensive review.

Torreya grandis (TG) nuts are highly valued for their rich content of bioactive compounds including lipids, proteins, carbohydrates, phenolic compounds, vitamins, and minerals, credited with numerous health benefits. In addition to their use in various culinary applications, such as baked goods and snacks, TG nuts are valuable source of high-quality oil rich in ω-3 and ω-6 unsaturated fatty acids, which has been shown to have anti-obesity, neuroprotective, and anti-diabetes effects. Besides, the byproducts from TG nuts processing, like the fruit aril and oil cake, are valuable sources of essential oils and proteins, respectively, with notable antioxidant, antimicrobial, and antifungal properties.

Effects of Nutrition Intensification on the Secondary Ovary Development and Oviposition of Redclaw Crayfish.

To further explore the impact of nutrient fortification on the ovarian secondary development of redclaw crayfish, four groups were set up: group ①, no feeding; group ②, formulated feeding; groups ③ and ④, formulated feed + nutrient bait. Results showed (1) the proportions of egg-bearing shrimp in groups ③ and ④. 2) The weight growth rate of group ① was the lowest, the maturation coefficient of unripe shrimp in group ④ was the highest, and the egg-holding rates in groups ③ and ④ were significantly higher than those of group ①.

Urine Sediment Detection Algorithm Based on Channel Enhancement and Deformable Convolution.

Urine sediment detection is a vital method in clinical urine analysis for evaluating an individual's kidney and urinary system health, as well as identifying potential diseases. Nevertheless, urine sediment images exhibit the characteristic of diverse shapes for the same category of targets. These characteristics pose a considerable challenge to the accurate identification of the visible components within the images.

Immunoprotective effect of recombinant Lactobacillus plantarum expressing largemouth bass virus MCP on largemouth bass.

Largemouth bass virus (LMBV) is an infectious pathogen that causes high mortality rates in largemouth bass, and outbreaks of this virus can significantly harm the aquaculture industry. Currently, no vaccine has been developed that can effectively prevent the transmission of LMBV. In this study, we constructed a recombinant Lactobacillus plantarum (L.

Research Progress on Antioxidant Peptides from Fish By-Products: Purification, Identification, and Structure-Activity Relationship.

Excessive reactive oxygen species (ROS) can lead to oxidative stress, which has become an urgent problem requiring effective solutions. Due to the drawbacks of chemically synthesized antioxidants, there is a growing interest in natural antioxidants, particularly antioxidant peptides. : By reviewing recent literature on antioxidant peptides, particularly those extracted from various parts of fish, summarize which fish by-products are more conducive to the extraction of antioxidant peptides and elaborate on their characteristics.

Three in one: A multifunctional oxidase-mimicking Ag/MnO nanozyme for colorimetric determination, precise identification, and broad-spectrum inactivation of foodborne pathogenic bacteria.

A multifunctional oxidase-mimicking Ag/MnO was prepared, catalyzing the 3, 3', 5, 5'-tetramethylbenzidine (TMB) chromogenic reaction. Six foodborne pathogenic bacteria species, including Escherichia coli, Staphylococcus aureus, Salmonella enterica, Listeria monocytogenes, Bacillus cereus, and Cronobacter sakazakii, were observed to differentially inhibit its oxidase-like activity, resulting in decelerating the TMB chromogenic reaction. Owing to these properties, the following achievements were achieved: colorimetric determination of these bacteria with high sensitivity can be achieved using Ag/MnO + TMB reaction system; precise identification of these bacteria at different concentrations, including individual bacterium, binary mixtures, and even multivariate mixtures, can be effectively realized by combining the Ag/MnO-based colorimetric sensor array with principal component analysis (PCA); broad-spectrum inactivation of these bacteria can be remarkably realized through catalyzation of Ag/MnO to generate superoxide anion free radicals.

A Chromosome-level genome assembly of giant river prawn (Macrobrachium rosenbergii).

The giant river prawn (Macrobrachium rosenbergii) is one of the most widely cultured crustacean species. In recent years, its aquaculture has faced challenges, including the degradation of germplasm resources and the emergence of viral diseases. Genomic information can be a valuable resource for developing molecular breeding programs for this important aquaculture species.

Colorimetric detection of Staphylococcus aureus with enhanced sensitivity based on phage covalently immobilized CoO nanozyme through synergistic inhibition effect.

Staphylococcus aureus (S. aureus) is a common foodborne pathogen, posing a serious threat to public health. Consequently, it is crucial to establish a platform for sensitive and specific determination of S.

Effect of different feeding regimens on physiological indicators, intestinal transcriptome, and bacterial flora of mandarin fish (Siniperca chuatsi).

Mandarin fish (Siniperca chuatsi) represents a typical carnivorous freshwater economic fish in China. Recently, the study of their feeding behavior to acclimate formulated diets has become a research focus. This study evaluated the effects of various diets on the body composition, nutritional content, digestive enzyme activity, gene expression, and gut microbiota of mandarin fish.

Genome assembly of redclaw crayfish (Cherax quadricarinatus) provides insights into its immune adaptation and hypoxia tolerance.

Background: The introduction of non-native species is a primary driver of biodiversity loss in freshwater ecosystems. The redclaw crayfish (Cherax quadricarinatus) is a freshwater species that exhibits tolerance to hypoxic stresses, fluctuating temperatures, high ammonia concentration. These hardy physiological characteristics make C.

Lipid Regulatory Element Interact with on Mitochondrial Bioenergetics in Bovine Adipocyte Differentiation and Lipometabolism.

The gene is a critical factor in animal physiological processes and has been shown to affect insulin resistance and fat accumulation in mammals. Nevertheless, little research has been conducted on its precise functions in lipid metabolism and adipogenic differentiation in beef cattle. This study analyzed the expression of and miR-199a-3p during bovine preadipocyte differentiation.

Identification of COMMD gene family in large yellow croaker (Larimichthys crocea): Immune response induced by Pseudomonas plecoglossicida infection and acute hypoxia stress.

The COMMD (Copper Metabolism gene MURR1 Domain) gene family consists of 10 members, which are involved in various biological processes such as copper and sodium transport, NF-κB activity and cell cycle progression. However, the study of COMMD gene family in large yellow croaker (Larimichthys crocea) is largely unknown. In this study, 10 COMMD gene family members (named LcCOMMDs) were successfully identified from large yellow croaker.

Inhibition of Fusarium graminearum growth and spore germination by a Streptomyces amritsarensis strain capable of killing and growing on Microcystis scum.

Aims: Developing energy-saving and ecofriendly strategies for treating harvested Microcystis biomass.

Methods And Results: Streptomyces amritsarensis HG-16 was first reported to effectively kill various morphotypes of natural Microcystis colonies at very high cell densities. Concurrently, HG-16 grown on lysed Microcystis maintained its antagonistic activity against plant pathogenic fungus Fusarium graminearum.

Regulation and Response Mechanism of Acute Low-Salinity Stress during Larval Stages in Based on Multi-Omics Analysis.

is an essential species for freshwater economic aquaculture in China, but in the larval process, their salinity requirement is high, which leads to salinity stress in the water. In order to elucidate the mechanisms regulating the response of to acute low-salinity exposure, we conducted a comprehensive study of the response of exposed to different salinities' (0‱, 6‱, and 12‱) data for 120 h. The activities of catalase, superoxide dismutase, and glutathione peroxidase were found to be significantly inhibited in the hepatopancreas and muscle following low-salinity exposure, resulting in oxidative damage and immune deficits in .

Genome-wide analysis reveals the contributors to fast molecular evolution of the Chinese hook snout carp ().

Variations in molecular evolutionary rate have been widely investigated among lineages and genes. However, it remains an open question whether fast rate of molecular evolution is driven by natural selection or random drift, and how the fast rate is linked to metabolic rate. Additionally, previous studies on fast molecular evolution have been largely restricted to concatenated matrix of genes or a few specifically selected genes, but less is known for individual genes at the genome-wide level.