Lignin and Peptide Promote the Abundance and Activity of Arsenic Methylation Microbes in Paddy Soils.

Rice physiological straighthead disease is induced by microbially mediated arsenic methylation and usually regionally distributed in paddy soils. However, the biogeochemical mechanism underlying the geographic distribution of microbial communities harboring methylating genes () remains unclear. Herein, we revealed significant ( = 0.

Sorbitol induces flavonoid accumulation as a secondary signal via the nanoencapsulated SPc/lncRNA809-MmNAC17 module against Alternaria alternata in Malus micromalus.

Sorbitol is an important primary metabolite that serves as both a carbon source and signal to pathogens. The leaf diseases caused by Alternata alternata are particularly serious in crabapple (Malus micromalus). Here, we found that sorbitol can enhance the resistance of crabapple to A.

Tomato B-cell lymphoma2 (Bcl2)-associated athanogene 5 (SlBAG5) contributes negatively to immunity against necrotrophic fungus Botrytis cinerea through interacting with SlBAP1 and modulating catalase activity.

The evolutionarily conserved and multifunctional B-cell lymphoma2 (Bcl2)-associated athanogene proteins (BAGs), serving as co-chaperone regulators, play a pivotal role in orchestrating plant stress responses. In this study, the possible involvement of tomato SlBAG genes in resistance to Botrytis cinerea was examined. The SlBAG genes respond with different expression change patterns to B.

Optimizing storage conditions for 'Harlikar' apples: The role of 1-methylcyclopropene and harvest stage.

The 'Harlikar' apple, known for its larger fruit and rich flavor, faces challenges in post-harvest storage. Our study compared the storage characteristics of 'Harlikar' apples at three different harvest stages and found that stage II showed superior storage characteristics. At stage II, the application of 1-methylcyclopropene (1-MCP) proved particularly beneficial, resulting in the slowest decline in apple firmness.

Impact of the drying process on the flavor characteristics of two economically important laver species, Pyropia haitanensis and Neopyropia yezoensis.

This study investigated the impact of drying process on the flavor profiles of the economically significant Pyropia haitanensis and Neopyropia yezoensis, aiming to clarify how species differences and drying process influence volatile and non-volatile flavor compounds. Using GC × GC-TOFMS and LC-MS, the volatile metabolites, free amino acids (FAAs), and nucleotides were analyzed. A total of 2774 volatile components, including 76 odor-active compounds (OACs), were identified, with aldehydes, ketones, benzenoids, and pyrazines as dominant contributors.

Notoginsenoside R1 alleviates blue light-induced corneal injury and wound healing delay by binding to and inhibiting TRIB1.

Background: With the escalating use of digital devices, blue light (BL) exposure has emerged as a critical concern due to its potential to cause ocular damage. This study explores the protective effects of notoginsenoside R1 (NR1), a bioactive compound from Panax notoginseng (Burkill) F.H.

Phthalate esters decreased nutritional value of rice grains via redirecting glycolytic carbon flow from grain quality formation toward antioxidative defense.

The prevalence and persistence of phthalate esters (PAEs) in agricultural soils has garnered global attention. Assessing their potential impacts on crop yield and quality necessitates a thorough understanding of their risks. In this study, we elucidated the carbon flow-dependent mechanisms of the decreased grain quality upon exposure to PAEs through a soil-based rice cultivation experiment.

Global identification and regulatory network analysis reveal the significant roles of lncRNAs during anther and pollen development in Arabidopsis.

A high-throughput sequencing identified 1283 lncRNAs in anthers at different stages in Arabidopsis and their relationship with protein-coding genes and miRNAs during anther and pollen development were analyzed. Long non-coding RNAs (lncRNAs) are important regulatory molecules involved in various biological processes. However, their roles in male reproductive development and interactions with miRNAs remained elusive.

In-Cell Mass Spectrometry and Ultraviolet Photodissociation Navigates the Intracellular Protein Heterogeneity.

Directly probing the heterogeneous conformations of intracellular proteins within their native cellular environment remains a significant challenge in mass spectrometry (MS). Here, we establish an in-cell MS and ultraviolet photodissociation (UVPD) strategy that directly ejects proteins from living cells into a mass spectrometer, followed by 193 nm UVPD for structural analysis. Applying this approach to calmodulin (CaM), we reveal that it adopts more extended conformations within living cells compared with purified samples , highlighting the unique influence of intracellular environments on protein folding.

Convergent evidence for the temperature-dependent emergence of silicification in terrestrial plants.

Research on silicon (Si) biogeochemistry and its beneficial effects for plants has received significant attention over several decades, but the reasons for the emergence of high-Si plants remain unclear. Here, we combine experimentation, field studies and analysis of existing databases to test the role of temperature on the expression and emergence of silicification in terrestrial plants. We first show that Si is beneficial for rice under high temperature (40 °C), but harmful under low temperature (0 °C), whilst a 2 °C increase results in a 37% increase in leaf Si concentrations.

Effect of soybean phosphatidylethanolamine-tamarind gum Maillard conjugate on physicochemical stability of water-in-oil emulsions.

Lipid oxidation hinders the development of water-in-oil (W/O) emulsions. This work aimed to determine the impact of soybean phosphatidylethanolamine (SP)/tamarind gum (TG) ratios on interface activity and anti-oxidant capacity of Maillard conjugates (MCs) in W/O emulsions. Results showed that grafting degree of MCs reached maximum with SP/TG ratio at 1:1 (43.

Plant-microbiome interactions for enhanced crop production under cadmium stress: A review.

Cadmium (Cd) is a toxic heavy metal that has detrimental effects on agriculture crops and human health. Both natural and anthropogenic processes release Cd into the environment, elevating its contents in soils. Under Cd stress, strong plant-microbiome interactions are important in improving crop production, but a systematic review is still missing.

Research note: Detection of IGF2BP2 gene polymorphism associated with growth and reproduction traits of pigeon.

The Insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) gene is closely associated with the diseases, fat deposition, and growth and reproduction traits in animals. Investigations into the relationship between the IGF2BP2 gene polymorphism and growth and reproduction traits provide valuable breeding information for enhancing productivity and economic gains in pigeon. In this study, the single nucleotide polymorphisms (SNPs) in 13 exons and the 3' untranslated region (3'UTR) of the IGF2BP2 gene in 292 female pigeons Tianxiang 1 strain were detected through Sanger sequencing, and their association with pigeons' growth and reproduction traits were explored.

Enantioselection behaviors and risk assessments of chiral pesticide ethiprole and its chiral metabolite ethiprole amide in five kinds of vegetables.

Ethiprole is a second-generation phenylpyrazole insecticide used in agricultural production as an alternative to fipronil due to its lower toxicity to bees. Ethiprole amide is chiral metabolite of ethiprole, but information regarding its formation and degradation in vegetables is limited. Here, the absolute configuration of ethiprole amide enantiomer was determined through circular dichroism, and the behaviors of chiral ethiprole and its metabolites in five kinds of vegetables were studied through field experiments.

Mining versatile feruloyl esterases: phylogenetic classification, structural features, and deep learning model.

Feruloyl esterases (FEs, EC 3.1.1.

A phytocytokine and its derived peptides in the frass of an insect elicit rice defenses.

Upon recognizing elicitors derived from herbivores, many plants activate specific defenses. Most of the elicitors identified thus far are from the oral secretions and egg-laying fluids of herbivores; in contrast, herbivore fecal excreta have been sparsely studied in this context. In this study, we identified elicitors in the frass of the striped stem borer (SSB; Chilo suppressalis) larvae using a combination of molecular and chemical analyses, bioactivity tests and insect performance bioassays.

Gut Microbiome and Metabolome Changes in Chronic Low Back Pain Patients With Vertebral Bone Marrow Lesions.

Background: Chronic low back pain (LBP) is a significant global health concern, often linked to vertebral bone marrow lesions (BML), particularly fatty replacement (FR). This study aims to explore the relationship between the gut microbiome, serum metabolome, and FR in chronic LBP patients.

Methods: Serum metabolomic profiling and gut microbiome analysis were conducted in chronic LBP patients with and without FR (LBP + FR,  = 40; LBP,  = 40) and Healthy Controls (HC,  = 31).

A multilayered regulatory network mediated by protein phosphatase 4 controls carbon catabolite repression and de-repression in Magnaporthe oryzae.

Carbon catabolite repression (CCR) and de-repression (CCDR) are critical for fungal development and pathogenicity, yet the underlying regulatory mechanisms remain poorly understood in pathogenic fungi. Here, we identify a serine/threonine protein phosphatase catalytic subunit, Pp4c, as essential for growth, conidiation, virulence, and the utilization of carbohydrates and lipids in Magnaporthe oryzae. We demonstrate that the protein phosphatase 4 complex (Pp4c and Smek1 subunits), the AMP-activated protein kinase (AMPK) Snf1, and the transcriptional regulators CreA (repressor) and Crf1 (activator) collaboratively regulate the utilization of non-preferred carbon sources.

Alternative splicing of vascular calcification: Insights, opportunities, and challenges.

Vascular calcification(VC) significantly increases the risk of cardiovascular events, leading to thickening of the myocardium and arteries, coronary heart disease, heart failure, and potentially triggering myocardial infarction and sudden cardiac death. Although VC is a reversible process, there are currently no methods or medications in clinical practice that can completely reverse or cure it. The current treatment strategies primarily focus on slowing the progression of VC and exploring new diagnostic and therapeutic approaches, making the identification of early diagnostic markers for VC particularly important.

Health risk assessment of dietary cadmium exposure based on cadmium bioavailability in food: Opportunities and challenges.

Long-term exposure to Cd through contaminated food can lead to multiple adverse health effects on humans. Although previous studies have covered global food Cd concentrations and dietary Cd exposures across different populations, there are increasing concerns regarding the adequacy of current food Cd safety standards to protect populations from adverse health effects. Moreover, incorporation of Cd relative bioavailability (Cd-RBA) in foods improves the accuracy of health risk assessment.

Carnivorous fishes can possess higher mercury levels than omnivorous fishes: A selective detection and efficient removal strategy for mercury using magnetic melamine-platinum composites fabricated by supermolecular self-assembly.

Mercury (II) ion (Hg) as highly toxic heavy metal may be accumulated in aquatic ecosystems and animals species so as to enter human body to conduct health harm. To ensure the safety of fishes food, hence, it is of great interest to evaluate the Hg levels in different kinds of fishes as well as Hg removal in aquaculture tailwater. In this article, a selective colormetric detection and efficient removal strategy has been developed for Hg ions by the controlled supermolecular self-assembly of melamine (MA)-platinum (Pt) composites onto mesoporous FeO carriers.

First Report of Causing Black Leaf Spot on in China.

Chinese yam ( Turcz.), known for its nutrient-rich underground tubers, is both a food source and a traditional Chinese medicinal plant. It offers significant nutritional and medicinal benefits.

The Genomics Revolution Drives a New Era in Entomology.

Thanks to the fast development of sequencing techniques and bioinformatics tools, sequencing the genome of an insect species for specific research purposes has become an increasingly popular practice. Insect genomes not only provide sets of gene sequences but also represent a change in focus from reductionism to systemic biology in the field of entomology. Using insect genomes, researchers are able to identify and study the functions of all members of a gene family, pathway, or gene network associated with a trait of interest.

Chrysanthemum extract mitigates high-fat diet-induced inflammation, intestinal barrier damage and gut microbiota disorder.

An effective intervention for obesity without side effects is needed. Chrysanthemum may be the preferred choice due to its influence in the improvement of glycolipid metabolism. This study assessed the efficacy of chrysanthemum and its flavonoids in mitigating high-fat diet (HFD) induced obesity, focusing on the integrity of the intestinal barrier, inflammation, and gut microbiota.

Measurement of multidifferential cross sections for dijet production in proton-proton collisions at .

A measurement of the dijet production cross section is reported based on proton-proton collision data collected in 2016 at by the CMS experiment at the CERN LHC, corresponding to an integrated luminosity of up to 36.3 . Jets are reconstructed with the anti- algorithm for distance parameters of and 0.