Polarity-sensitive pyrene fluorescent probes for multi-organelle imaging in living cells.

Polarity-sensitive probes (PAS) were synthesized through the attachment of azetidine and sulfonyl substituents to the pyrene fluorescent core. The emission peaks and fluorescence lifetimes of these PAS probes exhibit high sensitivity to polarity, enabling the visualization of microenvironmental characteristics and dynamics across multiple organelles.

Lumbar intervertebral disc calcification with massive herniation: a case report.

An 18-year-old female patient presented with a 1-month history of low back pain, which had worsened and was accompanied by radiating pain in the right lower limb for half a month. She was admitted to our hospital with computed tomography and magnetic resonance imaging findings suggesting calcification of the L3/4 disc and a large intraspinal mass at the L2-4 level. The patient's symptoms did not improve with conservative treatment, and her muscle strength rapidly declined.

BMP4-GPX4 can improve the ferroptosis phenotype of retinal ganglion cells and enhance their differentiation ability after retinal stem cell transplantation in glaucoma with high intraocular pressure.

Activation of bone morphogenetic protein (BMP) 4 signaling promotes the survival of retinal ganglion cell (RGC) after acute injury. In this study, we investigated the role of the BMP4 signaling pathway in regulating the degeneration of retinal ganglion cells (RGCs) in a mouse glaucoma model and its potential application in retinal stem cell. Our results demonstrate that BMP4-GPX4 not only reduces oxidative stress and iron accumulation but also promotes neuroprotective factors that support the survival of transplanted RSCs into the host retina.

Radiation knowledge and anxiety levels among residents proximity to the world's first AP1000 nuclear power unit.

Objective: Assess the level of radiation-related knowledge (RRK) and nuclear energy-related knowledge (NERK) among residents near the Sanmen Nuclear Power Plant, the first project adopted the Advanced Passive Pressurized Water Reactor (AP1000) technology.

Methods: In this study, respondents were selected using stratified multi-stage random sampling for residents aged 18 years and above living within 30 kilometers of the Sanmen Nuclear Power Station. Respondents were surveyed face-to-face by investigators who received standardized training.

IS-flanked composite transposon harboring in multidrug-resistant Typhimurium.

Multidrug-resistant Typhimurium has emerged as a global public health concern. Asymptomatic gastrointestinal carriage is a key factor in the spread of antibiotic-resistant bacteria. However, it is challenging to obtain direct evidence of transfer of mobile genetic elements (MGEs).

Integrating AI·IoT-OAHPs with existing elderly care systems.

This letter addresses the integration of artificial intelligence and the Internet of Things-based older adult healthcare programs with existing community and institutional elderly care systems. It highlights the current disconnect leading to service duplication and resource inefficiencies, proposes multifaceted integration approaches, and underscores the importance of supportive policies. International examples are referenced to demonstrate successful models, emphasizing the need for coordinated care to enhance service delivery and optimize resource use.

Gut Microbiome and Metabolome Changes in Chronic Low Back Pain Patients With Vertebral Bone Marrow Lesions.

Background: Chronic low back pain (LBP) is a significant global health concern, often linked to vertebral bone marrow lesions (BML), particularly fatty replacement (FR). This study aims to explore the relationship between the gut microbiome, serum metabolome, and FR in chronic LBP patients.

Methods: Serum metabolomic profiling and gut microbiome analysis were conducted in chronic LBP patients with and without FR (LBP + FR,  = 40; LBP,  = 40) and Healthy Controls (HC,  = 31).

Prediction of posthepatectomy liver failure in patients with hepatocellular carcinoma through ultrasound elastography.

In this article, we comment on the article by Cheng published in recently. Posthepatectomy liver failure (PHLF) remains a leading cause of hepatectomy-related mortality and can be evaluated according to liver reserve function. Liver stiffness (LS) measured by ultrasonic elastography and spleen area demonstrate a strong correlation with hepatic proliferation, fibrosis, and portal vein congestion, thus indirectly reflecting liver reserve function.

Exploring the noncovalent interaction between β-lactoglobulin and flavonoids under nonthermal process: Characterization, physicochemical properties, and potential for lycopene delivering.

The poor structure stability and low bioavailability of lycopene (LY) hampers the wide application in food field. Thus, it is crucial to explore novel deliver carrier for LY based on protein-flavonoid complexes. In this study, the noncovalent interaction mechanism between β-lactoglobulin (β-LG) and flavonoids (apigenin (API), luteolin (LUT), myricetin (MY), apigenin-7-O-glucoside, luteolin-7-O-glucoside, and myricetrin) under ultrasound treatment was explored.

Plinabulin exerts an anti-proliferative effect via the PI3K/AKT/mTOR signaling pathways in glioblastoma.

Objectives: Plinabulin, a marine-derived anticancer drug targeting microtubules, exhibits anti-cancer effects on glioblastoma cells. However, its therapeutic potential, specifically for glioblastoma treatment, remains underexplored. This study aims to elucidate the mechanisms by which plinabulin exerts its effects on glioblastoma cells.

Ultra-small curcumin-ruthenium coordination polymer nanodots prevent renal ischemia-reperfusion injury and the progression to chronic kidney disease.

Renal ischemia-reperfusion (IR) induces tissue hypoxia, resulting in disrupted energy metabolism and heightened oxidative stress. These factors contribute to tubular cell damage, which is a leading cause of acute kidney injury (AKI) and can progress to chronic kidney disease (CKD). The excessive generation of reactive oxygen species (ROS) plays a crucial role in the pathogenesis of AKI.

Regulation of involucrin and signalling pathway in scleroderma epidermal keratinocytes.

Introduction: Systemic sclerosis is a complex disease characterized by the fibrosis and vasculopathy.

Aim: We aimed to assess scleroderma by examining involucrin, an early terminal differentiation marker of epidermal keratinocytes.

Material And Methods: Immunolocalization of involucrin was performed in healthy controls and patients with scleroderma lesions by using an immunofluorescence (IF) assay.

Association of CSF soluble TREM1 levels with hippocampal atrophy in cognitively impaired older adults.

Background: Recent studies have shown that cerebrospinal fluid (CSF) levels of soluble triggering receptor expressed on myeloid cells 1 (sTREM1) are elevated in individuals with Alzheimer's disease (AD), though the relationship between CSF sTREM1 and hippocampal atrophy remains to be elucidated. The primary aim of this study was to investigate the association between CSF sTREM1 levels and longitudinal changes in hippocampal volumes, and to determine if this relationship is moderated by cognitive status.

Methods: We included 576 participants, comprising 152 cognitively unimpaired (CU) and 424 cognitively impaired (CI) individuals.

Silent cerebral lesions after catheter ablation for atrial fibrillation using cryoballoon, hotballoon, laserballoon and radiofrequency catheters: a Bayesian network meta-analysis.

Background: Catheter ablation (CA) is an effective therapeutic option for patients with symptomatic atrial fibrillation (AF). Previous studies have reported silent cerebral lesions (SCLs) detected by magnetic resonance imaging (MRI) after different CA techniques; however, the results were controversial. Therefore, we performed this network meta-analysis (NMA) to assess the incidence of SCLs after cryoballoon, hotballoon, laserballoon, and radiofrequency ablation (RFA).

Neurotransmitters crosstalk and regulation in the reward circuit of subjects with behavioral addiction.

Behavioral addictive disorders (BADs) have become a significant societal challenge over time. The central feature of BADs is the loss of control over engaging in and continuing behaviors, even when facing negative consequences. The neurobiological underpinnings of BADs primarily involve impairments in the reward circuitry, encompassing the ventral tegmental area, nucleus accumbens in the ventral striatum, and prefrontal cortex.

Co-Delivery of Dacarbazine and miRNA 34a Combinations to Synergistically Improve Malignant Melanoma Treatments.

Purpose: The incidence of malignant melanoma (MM) has risen over the past three decades, and despite advancements in treatment, there is still a need to improve treatment modalities. This study developed a promising strategy for tumor-targeted co-delivery of Dacarbazine (DTIC) and miRNA 34a-loaded PHRD micelles (Co-PHRD) for combination treatment of MM.

Methods: To construct the dual drug-loaded delivery system Co-PHRD, poly (L-arginine)-poly (L-histidine)-polylactic acid (PLA) was employed as a building block.

Correlation between dental fluorosis risk and bone-specific alkaline phosphatase, osteocalcin, matrix metalloproteinase and parathyroid hormone in children.

Background: The aim of this study was to investigate the association between dental fluorosis occurrence in children and bone metabolism-related indicators, including bone-specific alkaline phosphatase (BALP), osteocalcin (OC), matrix metalloproteinase (MMP-2, MMP-9, MMP-20), and parathyroid hormone (PTH).

Methods: A total of 189 cases of school-age children who underwent health examinations in our hospital were enrolled, according to the presence or absence of dental fluorosis. They were divided into the fluorosis group (n=97) and fluoride-free group (n=92), and the serum BALP, OC, MMP-2, MMP-9, MMP-20, and PTH levels of the two groups were compared and relevant clinical data were collected.

Influence mechanism of serum free immunoglobulin light chain on pulmonary inflammatory response and serum levels of inflammatory factors in patients with chronic obstructive pulmonary disease.

Background: The pathogenesis of chronic obstructive pulmonary disease (COPD) is not fully understood. This work aimed to demonstrate the role of serum free light chains (sFLC) in the pulmonary inflammatory response of COPD patients and its relationship with serum inflammatory cytokine (IC) levels.

Methods: Eighty subjects were enrolled, including 40 COPD patients (COPD group) and 40 healthy controls (control group).

Case report: special imaging features in a uremic patient with intracranial infection caused by , almost misdiagnosed as demyelinating disease.

is a very rare pathogen that causes intracranial infection. It is commonly found in immunocompromised patients and is resistant to multiple antibiotics. In this case report, we present a case of human central nervous system infection caused by , which was initially misdiagnosed as demyelinating disease due to the specific imaging findings.

Ocular inoculation of toad venom: toxic cataract and proteomic profiling.

Purpose: To report a singular case of cataract caused by toad venom inoculation and to scrutinize the pathological mechanisms through proteomic sequencing of the lens specimen.

Methods: A young Chinese male presented with progressively deteriorating vision in his right eye subsequent to a history of toad venom inoculation. He was diagnosed with a toxic cataract, and underwent phacoemulsification cataract surgery.

Temperature-Enhanced Purine Metabolism-Based Versatile SERS Platform for Rapid Clinical Pathogens Diagnosis and Drug-Resistant Assessment.

Label-free surface-enhanced Raman spectroscopy (SERS) combined with machine learning (ML) techniques presents a promising approach for rapid pathogen identification. Previous studies have demonstrated that purine degradation metabolites are the primary contributors to SERS spectra; however, generating these distinguishable spectra typically requires a long incubation time (>10 h) at room temperature. Moreover, the lack of attention to spectral variations between strains of the same bacterial species has limited the generalizability of ML models in real-world applications.

Response of an obligate CAM plant to competition and increased watering intervals.

Climate change has exacerbated precipitation variability, profoundly impacting vegetation dynamics and community structures in arid ecosystems. There remains a notable knowledge gap regarding the ecological effects of altered precipitation on crassulacean acid metabolism (CAM) plants and their interactions with other photosynthetic types. This study investigated the response of the typical obligate CAM plant Orostachys fimbriata to extended watering intervals (WI4-WI8) and various competitive patterns (M-M) with the C grass Melilotus officinalis and the C grass Setaria viridis through greenhouse experiments.

Every Face Has a Name: Individuation Training Reduces Implicit Racial Bias.

Addressing racial bias in early childhood is crucial for fostering inclusivity and reducing social inequalities. This study examined the effectiveness of individuation training in reducing racial bias among Canadian preschool-aged children and explored how interracial contact might influence changes in children's implicit anti-Black bias. A total of 113 preschool-age children (60 females, M = 5.