Sensitive phosphorimetric determination of bumetanide in human urine with its inhibition effect on the formation of [Fe-morin]3+ complex.

A novel solid substrate-room temperature phosphorimetry (SS-RTP) was developed for determination of bumetanide (BMTN). It was validated by determining selectivity, linearity, accuracy, precision, and signal to noise ratio (S/N) for analysis. And all the experiments presented in this work were based on that BMTN inhibited the formation of [Fe-morin](3+) ([FeR](3+)) complex by the reaction between Fe(3+) and R, which led to severe quenching of room temperature phosphorescence (RTP) signal.

8-Quinolineboronic acid as a potential phosphorescent molecular switch for the determination of alpha-fetoprotein variant for the prediction of primary hepatocellular carcinoma.

8-Quinolineboronic acid phosphorescent molecular switch (8-QBA-PMS) in the "off" state emitted weak room temperature phosphorescence (RTP) of 8-QBA on the acetylcellulose membrane (ACM) with the perturbation of Pb(2+). When 8-QBA-PMS was used to label concanavalin agglutinin (Con A) to form 8-QBA-PMS-Con A based on the reaction between -OH of 8-QBA-PMS and -COOH of Con A, 8-QBA-PMS turned "on" automatically due to its structure change, and RTP of the system increased 2.7 times.

Exploitation of phosphorescent labelling reagent of fullerol-fluorescein isothiocyanate and new method for the determination of trace alkaline phosphatase as well as forecast of human diseases.

A new phosphorescent labelling reagent consisting of fullerol, fluorescein isothiocyanate and N,N-dimethylaniline (F-ol-(FITC)(n)-DMA) was developed. The mode of action is based on the reactivity of the active -OH group in F-ol with the -COOH group of FITC to form an F-ol-(FITC)(n)-DMA complex containing several FITC molecules. F-ol-(FITC)(n)-DMA increased the number of luminescent molecules in the biological target of WGA-AP-WGA-F-ol-(FITC)(n)-DMA (WGA and AP are wheat germ agglutinin and alkaline phosphatase, respectively) which improved the sensitivity using solid substrate room temperature phosphorimetry (SSRTP) detection.

Solid substrate-room temperature phosphorimetry for the determination of trace protein using ion association complex [Cu(BPY)2]2+ . [(Fin)2]2- as a phosphorescent probe.

Fluorescein (HFin) emitted strong and stable room temperature phosphorescence (RTP) on filter paper after set at 50 degrees C for 10 min using Li(+) as the ion perturber. HFin existed as Fin(-) when the pH value was in the range of 5.45-7.

Solid substrate-room temperature phosphorimetry for the determination of residual clenbuterol hydrochloride based on the catalysis of sodium periodate oxidizing eosine Y.

Clenbuterol hydrochloride (CLB) could catalyze NaIO(4) oxidation of eosine Y (R), which caused the room temperature phosphorescence (RTP) signal of R to quench sharply. The DeltaI(P) (= I(P2)-I(P1), I(P2) was RTP intensities of reagent blank and I(P1) was RTP intensities of test solution) of the system was directly proportional to the content of CLB. According to that academic thought, a new solid substrate-room temperature phosphorimetry (SS-RTP) for the determination of trace CLB has been established.

Determination of trace mercury by solid substrate room temperature phosphorescence quenching method based on lead carboxymethyl cellulose (Pb(CMC)(2)) particles containing luminescent salicyl fluorones molecules.

Luminescent particles of lead carboxymethyl cellulose (Pb(CMC)(2)), which contains salicyl fluorones (THBF), Pb(CMC)(2)-THBF, were synthesized by sol-gel method. Pb(CMC)(2)-THBF can emit intense and stable solid substrate room temperature phosphorescence (SS-RTP) on filter paper. EDTA can chelate the Pb(2+) in Pb(CMC)(2)-THBF, causing it decompose into aqueous soluble components PbY(2-), CMC(-) and THBF, and these components can react with Hg(2+) to form (CMC)(2)Hg-THBF, causing decrease of phosphorescence intensity.

Determination of trace mercury by solid substrate-room temperature phosphorimetry based on catalytic effect of Hg(2+) on formation of the ion association complex [Fe(bipy)(3)](2+)[(FinBr(4))(2)](2-).

A new solid substrate-room temperature phosphorimetry method for the determination of trace mercury has been established. It bases on the fact that in acetic acid medium, Hg(2+) ion can catalyze the substitute reaction of CN(-) ligand in [Fe(CN)(6)](4-) by 2,2'-bipyridyl (bipy), and the resultant [Fe(bipy)(3)](2+) cation can react with FinBr(4)(-) anion of tetrabromofluorescein (HFinBr(4)) to form ion association complex [Fe(bipy)(3)](2+)[(FinBr(4))(2)](2-) which can emit phosphorescent signal on filter paper substrate. Under the optimum condition, the linear dynamic range of this method is 1.

Determination of trace alpha-fetoprotein variant by affinity adsorption solid substrate-room temperature phosphorimetry.

The 3.5-generation dendrimers (3.5G-D)-porphyrin (P) dual luminescent molecule (3.

Determination of bioactive matter by affinity adsorption solid substrate-room temperature phosphorimetry based on lectin labeled with self-ordered ring of eosin Y.

A new phosphorescent labeling reagent named self-ordered ring (ESOR) of eosin Y (E) was developed. And the application of the determination of bioactive matter by affinity adsorption solid substrate-room temperature phosphorimetry (AA-SS-RTP) based on ESOR labeling lectin was studied. Results showed that pink and homogeneous ESOR could be formed by E on polyamide membrane (PAM) in the presence of cetyltrimethylammonium bromide (CTAB) and ammonia water.

[Flow-injection chemiluminescence determination of procaterol hydrochloride].

A new flow injection chemiluminescences method for the determination of procaterol hydrochloride has been developed. It is based on the chemiluminescence reaction of procaterol hydrochloride with Ce (IV)-rhodamine B in sulphuric-chlorhydric acid medium and the experimental fact of the enhancement of chemiluminescence by surfactant CTMAB. The concentration of the analyte shows a good linear relationship with the produced luminescence intensity in the range of 2.

Development of a benzene vapour sensor utilizing chemiluminescence on Y2O3.

Chemiluminescence (CL) was observed when benzene vapour passed through the surface of Y(2)O(3), TiO(2), Y(2)O(3)-V(2)O(5), TiO(2)-Y(2)O(3), Y(2)O(3)-Cr(2)O(3), Y(2)O(3)-Al(2)O(3) and TiO(2)-Al(2)O(3), with air as the carrier gas. The strongest CL intensity was found with Y(2)O(3) as the catalyst. A novel benzene sensor based on this kind of CL was developed.

Determination of trace glucose and forecast of human diseases by affinity adsorption solid substrate-room temperature phosphorimetry based on triticum vulgaris lectin labeled with dendrimers-porphyrin dual luminescence molecule.

In this paper, 3.5-generation polyamidoamine dendrimers (3.5G-D)-porphyrin (P) dual luminescence molecule (3.

Solid substrate-room temperature phosphorimetry for the determination of trace terbutaline sulfate based on its inhibition oxidation of rhodamine 6G by sodium periodate.

When 1.00 mol l(-1) I(-) is used as ion perturber, rhodamine 6G (Rh 6G) can emit strong and stable room temperature phosphorescence (RTP) on filter paper substrate in KHC(8)H(4)O(4)-HCl buffer solution (pH = 3.50), heated at 70 degrees C for 10 min.

Determination of trace alpha-fetoprotein variant by affinity adsorption solid substrate-room temperature phosphorimetry and its application to the forecast of human diseases.

In the presence of ion perturber LiAc, 4-generation polyamidoamine dendrimers (4G-D) could emit strong and stable room temperature phosphorescence (RTP) signal at lambda(max)(ex)/lambda(max)(em) = 511.8/675.3 nm on nitrocellulose membrane (NCM), and Triton X-100 could sharply enhance the RTP signal of 4G-D.

Determination of trace formaldehyde by solid substrate-room temperature phosphorescence quenching method based on the rose bengal-potassium bromate-Tween-80 system.

A new method for the determination of trace formaldehyde by solid substrate-room temperature phosphorescence quenching method has been proposed. It is based on the facts that rose bengal (R) can emit intense and stable room temperature phosphorescence on the solid substrate of filter paper (SS-RTP). Potassium bromate (KBrO(3)) can oxidize R, which causes the quenching of RTP.

Solid substrate-room temperature phosphorescence method for the determination of trace Mn(II) based on oxidizing reaction of hydrogen peroxide using alpha,alpha'-bipyridine as sensitizer.

A new solid substrate-room temperature phosphorescence (SS-RTP) method for the determination of trace manganese (II) has been established. It bases on the fact that fullerol (R) emits strong and stable room temperature phosphorescence (RTP) on filter paper substrate. H2O2 can oxidize R to cause the SS-RTP quenching.

Determination of trace mercury by solid substrate-room temperature phosphorimetry quenching method based on catalytic effect of Hg2+ on formation of the ion association complex [Sn(XO)6]4+.[(Fin)4].

A new method for the determination of trace mercury by solid substrate-room temperature phosphorimetry (SS-RTP) quenching method has been established. In glycine-HCl buffer solution, xylenol orange (XO) can react with Sn4+ to form the complex [Sn(XO)6]4+. [Sn(XO)6]4+ can interact with Fin- (fluorescein anion) to form the ion associate [Sn(XO)6]4+.

Determination of trace selenium by solid substrate-room temperature phosphorescence enhancing method based on potassium chlorate oxidizing phenyl hydrazine-1,2-dihydroxynaphthalene-3,6-disulfonic acid system.

A new method for the determination of trace selenium based on solid substrate-room temperature phosphorimetry (SS-RTP) has been established. This method was based on the fact that in HCl-KCl buffer solution, potassium chlorate could oxidize phenyl hydrazine to form chloridize diazo-ion after being heated at 100 degrees C for 20 min, and then the diazo-ion reacted with 1,2-dihydroxynaphthalene-3,6-disulfonic acid to form red azo-compound which could emit strong room temperature phosphorescence (RTP) signal on filter paper. Selenium could catalyze potassium chlorate oxidizing the reaction between phenyl hydrazine and 1,2-dihydroxynaphthalene-3,6-disulfonic acid, which caused the sharp enhancement of SS-RTP.

Determination of trace lead by solid substrate room temperature phosphorescence enhancing method based on heavy atom effect and dissoluble manganese supramolecule containing rhodamine 6G luminescent particles.

Dissoluble manganese supramolecule containing rhodamine 6G luminescent particles (M2) are synthesized, based on dissoluble manganese supramolecule (M1) doping rhodamine 6G (R.6G), by crystalline method. The particle diameters of M1 and M2 determined by ETM are both of micron degree.

Determination of trace silver by solid substrate-room temperature phosphorescence quenching method based on double catalytic system of meta-nitrophenyfluorone-polyoxyethylene-chromium-potassium bromate-beta-cyclodextrin.

A new solid substrate-room temperature phosphorescence (SS-RTP) quenching method for the determination of trace silver has been established. It is based on the fact that when using Mg(2+) as ion perturber and beta-CD as surfactant, the system of meta-nitrophenyfluorone (R)-polyoxyethylene-Cr(III) can emit strong and stable room temperature phosphorescence signal on filter paper whose surface is modified by polyvinyl alcohol (PVA)-H(3)BO(3)-NaOH. Ag(I) can catalyze KBrO(3) oxidizing R-PEO-Cr(III) system which causes the quenching of SS-RTP.

Determination of trace copper by solid substrate-room temperature phosphorescence quenching method based on activating effect of alpha,alpha'-dipyridyl on Vitamin C reducing beryllon.

A new solid substrate-room temperature phosphorescence quenching method for the determination of trace copper has been established. It is based on the fact that beryllon (R) can emit strong and stable solid substrate-room temperature phosphorescence on the filter paper, and Vitamin C (Vc) reduces R to non-phosphorescent compound that leads to solid substrate-room temperature phosphorescence (SS-RTP) quenching of R, and alpha,alpha'-dipyridyl can activate copper catalyzing Vitamin C reducing R. The DeltaI(p) of the system with alpha,alpha'-dipyridyl is 3.

Solid-substrate room-temperature phosphorescence immunoassay based on an antibody labeled with nanoparticles containing dibromofluorescein luminescent molecules and analytical application.

Luminescent 20-nm silicon dioxide nanoparticles containing dibromofluorescein (D; particles denoted D-SiO(2)) were synthesized by the sol-gel method. In the presence of Pb(Ac)(2) as a heavy atom perturber, the particle can emit the intense and stable room temperature phosphorescence(RTP) signal on a polyamide membrane at the wavelength of lambdaex(max)/lambdaem(max) = 460/623 nm for D. Our research indicates that the specific immune reaction between goat-anti-human IgG antibody labeled with D-SiO(2) and human IgG can be carried out quantitatively on a polyamide membrane, and the phosphorescence intensity was evidently enhanced after the immunoreaction.

Determination of trace silver by solid substrate room temperature phosphorescence quenching method based on lead carboxymethyl cellulose particles (Pb(CMC)2) containing luminescent salicyl fluorenes molecules.

Luminescent particles of lead carboxymethyl cellulose (Pb(CMC)2) containing salicyl fluorones (THBF), Pb(CMC)2-THBF were synthesized by the sol-gel method, using sodium carboxymethyl cellulose (NaCMC) as precursor and Pb2+ as precipitant. Pb(CMC)2-THBF can emit the intense and stable solid substrate room temperature phosphorescence (SS-RTP) on filter paper. And EDTA can chelate Pb2+ in Pb(CMC)2-THBF, causing it to decompose into aqueous soluble components PbY2-, CMC- and THBF, which can react with Ag+ to form Ag(CMC)2-THBF, causing the decrease of phosphorescence intensity.

Determination of traces of bismuth by quenching of solid-substrate room-temperature phosphorescence from morin-labeled silicon dioxide nano-particles.

Silicon dioxide nano-particles, diameter 50 nm, containing morin (morin-SiO2) have been synthesized by the sol-gel method. They emit strong and stable room-temperature phosphorescence (SS-RTP) on filter paper as substrate, and bismuth can quench the intensity of the SS-RTP. On this basis a new morin-SiO2 solid-substrate room-temperature phosphorescence-quenching method has been established for determination of traces of bismuth.

[Chemiluminescence method for determination of dipyridamole].

A new system for the determination of dipyridamole with flow injection chemiluminescence was described. It is based on the chemiluminescence reaction of dipyridamole-potassium permanganate with rhodamine B. Tween-80 was found to be an enhancer of the chemiluminescence reaction.