Fine mapping of TFL, a major gene regulating fruit length in snake gourd (Trichosanthes anguina L).

Fruit length is a crucial agronomic trait of snake gourd (Trichosanthes anguina L); however, genes associated with fruit length have not been characterised. In this study, F snake gourd populations were generated by crossing the inbred lines, S and S (fruit lengths: 110 and 20 cm, respectively). Subsequently, bulk segregant analysis, sequencing, and fine-mapping were performed on the F population to identify target genes.

An artificial intelligence method for comprehensive evaluation of preschool education quality.

The evolution in the quality of teaching for preschool education is worth studying. In this article, we solved the qualitative problems in the comprehensive quality evaluation by suggesting a method of quantitative combination and establishing a set of indicators suitable for the comprehensive quality evaluation of students in the kindergarten. According to the experience summed up by previous scholars, the weight of each index is obtained by an analytic hierarchy process.

The further characterization of the peptide specifically binding to gastric cancer.

Targeting peptide has been considered to be useful as a small molecule probe leading to multifunctional properties for both imaging detection and targeting therapy. Thus, the identification of novel targets is urgently needed to develop innovative agents to effectively control gastric cancer metastasis and progression. Previously, we reported a novel 12-mer peptide, GP-5 (IHKDKNAPSLVP), binding to gastric carcinoma (GC) cells specifically and sensitively, and it was screened by using a phage displayed peptide library and primarily analyzed.

Screening of a specific peptide binding to esophageal squamous carcinoma cells from phage displayed peptide library.

To select a specifically binding peptide for imaging detection of human esophageal squamous cell carcinoma (ESCC), a phage-displayed 12-mer peptide library was used to screen the peptide that bind to ESCC cells specifically. After four rounds of bio-panning, the phage recovery rate gradually increased, and specific phage clones were effectively enriched. The 60 randomly selected phage clones were tested using cellular enzyme-linked immunosorbent assay (ELISA), and 41 phage clones were identified as positive clones with the over 2.

Room temperature ionic liquids enhanced the speciation of Cr(VI) and Cr(III) by hollow fiber liquid phase microextraction combined with flame atomic absorption spectrometry.

A new method for the speciation of Cr(VI) and Cr(III) based on enhancement effect of room temperature ionic liquids (RTILs) for hollow fiber liquid phase microextraction (HF-LPME) combined with flame atomic absorption spectrometry (FAAS) was developed. Room temperature ionic liquids (RTILs) and diethyldithiocarbamate (DDTC) were used enhancement reagents and chelating reagent, respectively. The addition of room temperature ionic liquids led to 3.

Antioxidant Activities of Plumbagin and Its Cu (II) Complex.

Plumbagin and its Cu (II) complex [Cu (plumbagin)(2)]·H(2)O have been synthesized, and their antioxidant activities towards the inhibitory effect on DPPH free radical, reducing power, total antioxidant capacity, and inhibition on lipid peroxidation were investigated. Plumbagin and its Cu (II) complex were found to exhibit scavenging activities on DPPH radical with the inhibitory rate of 41% and 24%, respectively. The reducing power of plumbagin was outstanding at the concentrations of 1.

PCR-DGGE as a supplemental method verifying dominance of culturable microorganisms from activated sludge.

To verify the dominance of microorganisms in wastewater biological treatment, PCR-DGGE (denaturing gradient gel electrophoresis) was performed as a supplementary support method for screening of the dominant microorganisms from activated sludge. Results suggest that the dominant microorganisms in activated sludge are primarily responsible for strengthening its effectiveness as a biological treatment system, followed by the non-main dominant microorganisms, whereas the non-dominant microorganisms showed no effects. The degree of microbial abundance present on the profile of PCR-DGGE was in line with the treatment efficiency of augmented activated sludge with isolated cultures, suggesting that PCR-DGGE can be used as an effective supplementary method for verifying culturable dominant microorganisms in activated sludge of coking wastewater.

[A comparison between the effects on DNA-protein cross-links of Saccharomyces and E. coli induced by formaldehyde].

To explore the effect of formaldehyde on DNA-protein cross-links (DPC) in eucaryotic cells and prokaryotic cells, Pichia pastoris and E. coli DH5alpha were chosen as materials to evaluate the amount of DPC induced by liquid formaldehyde in vivo by the method of KCl-SDS assay. The results showed that formaldehyde could not induce DPC at low dose(25micromol/L,P > 0.