High-throughput light sheet imaging of adult and larval Parkinson's disease model using a low-cost optofluidic device and a fluorescent microscope.

Recent advancements at the interface of microfluidics technology and light sheet fluorescence microscopy have opened the door for high-throughput and high-content investigation of disease models. In this paper, we report on the development of a simple, miniaturized, and low-cost optofluidic platform that can be added to a conventional inverted fluorescent microscope for continuous light sheet imaging of transgenic worm populations with high lateral and axial resolutions of 1.1 µm and 2.

COVID-19 Outbreaks in Canada and the Crisis of Migrant Farmworkers' Social Reproduction: Transnational Labour and the Need for Greater Accountability Among Receiving States.

In 2020, migrant farmworkers in Canada, cast as essential to sustaining the national food supply, experienced relatively high COVID-19 infection rates. Taking Southern Ontario as its focus, this article reveals how the federal government response to COVID-19 in agriculture perpetuated the effects of longstanding laws and policies requiring migrant farmworkers, circumscribed in their ability to politically mobilize on account of their institutionalized deportability, to shoulder disproportionate amounts of economic, social, and health risks. Centering the transnational character of migrant farmworkers' renewal, it identifies meaningful interventions to limit the structural disempowerment of migrant farmworkers and the externalization of their social reproduction.

Donor-acceptor-acceptor-type near-infrared fluorophores that contain dithienophosphole oxide and boryl groups: effect of the boryl group on the nonradiative decay.

The use of donor-π-acceptor (D-π-A) skeletons is an effective strategy for the design of fluorophores with red-shifted emission. In particular, the use of amino and boryl moieties as the electron-donating and -accepting groups, respectively, can produce dyes that exhibit high fluorescence and solvatochromism. Herein, we introduce a dithienophosphole -oxide scaffold as an acceptor-spacer to produce a boryl- and amino-substituted donor-acceptor-acceptor (D-A-A) π-system.

A proof of concept application of aptachain: ligand-induced self-assembly of a DNA aptamer.

A challenge for the use of aptamers as biosensors is how to signal the occurrence of their ligand binding event into a signal that can be exploited in a detection scheme. Here, we present the concept of "aptachain" formation, where an aptamer is split into two overlapping or staggered strands and assembles into an extended oligomer upon ligand binding. This assembly of aptamers can then be used as a way to detect ligand binding by the aptamer.

A revision of the cleptoparasitic bee genus Latreille for Nearctic species, north of Mexico (Hymenoptera, Apidae).

Herein, the cleptoparasitic (cuckoo) bee genus (Hymenoptera: Apidae) is revised for species occurring in North America, north of Mexico, and an updated checklist of all species known to occur in Canada and the United States of America is provided with comprehensive descriptions, diagnoses, and a single dichotomous key (using the same couplets for both sexes) to aid in their identification. To increase their recognition among North American naturalists, English common names are also proposed for all North American . A total of 43 species is confirmed as present in the region, 15 of which are newly recognized.

Distance measures and optimization spaces in quantitative fatty acid signature analysis.

Quantitative fatty acid signature analysis has become an important method of diet estimation in ecology, especially marine ecology. Controlled feeding trials to validate the method and estimate the calibration coefficients necessary to account for differential metabolism of individual fatty acids have been conducted with several species from diverse taxa. However, research into potential refinements of the estimation method has been limited.

Sweeping beauty: is grassland arthropod community composition effectively estimated by sweep netting?

Arthropods are critical ecosystem components due to their high diversity and sensitivity to perturbation. Furthermore, due to their ease of capture they are often the focus of environmental health surveys. There is much debate regarding the best sampling method to use in these surveys.