[Analysis of a Chinese pedigree with hereditary spherocytosis caused by intron variation of SPTB gene].

To analyze a novel intronic variant in the SPTB gene and explore its effect on SPTB mRNA splicing. Clinical data of a child diagnosed with hereditary spherocytosis (HS) and admitted to the First Affiliated Hospital of Xi'an Jiaotong University in February 2022 were analyzed retrospectively. Whole genome sequencing was used to identify disease-causing mutations and the results were validated with Sanger sequencing, mRNA sequencing was used to determine the SPTB gene's mRNA expression level, and bioinformatics tools were used for splicing site prediction and analysis.

[Analysis of colonization rate and molecular characteristics of on tracheotomy wounds at early postoperational stage in neonates].

To analyze the colonization rate and molecular types of (SA) on the tracheotomy wounds of neonates at early postoperative stage in neonatal intensive care unit (NICU). This was a case series study. Patients who were admitted and underwent tracheotomy in NICU of Beijing Children's Hospital, Capital Medical University from January 1 2020 to December 31 2023 were enrolled.

[Glycemic control and influencing factors in children with type 1 diabetes mellitus in Xinjiang].

To investigate the glycemic control status and analyze its influencing factors in children with type 1 diabetes mellitus (T1DM) at a single center in Xinjiang. A cross-sectional survey was conducted. The clinical data of children with T1DM who were admitted to the Pediatric Center of the First Affiliated Hospital of Xinjiang Medical University from January 2019 to December 2023 were collected, including their general information, course of disease, insulin administration method, and glycosylated hemoglobin (HbA1c) levels.

[Analysis of perinatal factors for death or severe intraventricular hemorrhage within the first week of life in preterm infants with gestational age <32 weeks].

To analyze the incidence and perinatal factors of death or severe intraventricular hemorrhage (sIVH) within the first week of life in preterm infants with gestational age <32 weeks. Based on the online data platform of Sina-northern Neonatal Network (SNN), a case-control study was conducted using clinical data from 8 903 preterm infants with gestational age <32 weeks admitted to 35 neonatal intensive care units (NICU) between 2019 and 2023. Infants were classified by gestational age at birth into very preterm infants and extremely preterm infants.

[A survey on the time to reach full enteral feeding for preterm infants with gestational age <32 weeks in neonatal intensive care units of Chinese Neonatal Network].

To analyze the time to reach full enteral feedings (TFEF) among preterm infants with gestational age (GA)<32 weeks admitted to the neonatal intensive care units (NICU) of Chinese Neonatal Network (CHNN). This was a retrospective analysis based on the database from the CHNN 89 participating centers between January 1, 2019 and December 31, 2022. All 16 155 preterm infants with a GA <32 weeks and a birth weight <1 500 g, admitted to the NICU within 24 h after birth, hospitalization for at least 7 d and achieved full enteral feedings before discharge were included.

[Clinical characteristics and therapeutic experience with rivaroxaban in children with pneumonia associated with pulmonary thromboembolism].

To evaluate the efficacy and safety of rivaroxaban and investigate the clinical features of pneumonia (MPP) associated with pulmonary thromboembolism (PTE) in children. A case series study was conducted on 36 children, diagnosed with MPP associated with PTE and hospitalized in our institution from January 2020 to June 2024 of Department of Pediatric Respiratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University. Clinical data and follow-up information were collected to analyze their clinical characteristics, outcomes, and adverse events to rivaroxaban.

[Infectious pulmonary artery pseudoaneurysms in 3 children and literatures review].

To summarize the clinical characteristics and prognosis of infectious pulmonary artery pseudoaneurysm (IPAP) in pediatric patients. This case series study summarizes the clinical data of 3 children diagnosed with IPAP at the Children's Hospital of Chongqing Medical University from January 2015 to December 2024.A comprehensive literature review was conducted by searching Chinese databases (China National Knowledge Infrastructure, Wanfang Data Knowledge Service Platform, China VIP Database and Chinese Medical Journals Full Text Database) and the PubMed database through December 2024, using the keywords"infect""pseudoaneurysm""pulmonary artery".

[Clinical features of pneumonia in 10 children].

To summarize the clinical features of pneumonia (CPP) in children. Case series study. Clinical data of 10 children with CPP hospitalized in Department No.

Exploring marking methods for the predatory hoverfly Sphaerophoria rueppellii (Diptera: Syrphidae).

Hoverflies are essential to ecosystems, with adults serving as important pollinators and larvae preying on plant-feeding insects or recycling nutrients. Species like Sphaerophoria rueppellii are used in biocontrol programs to target aphid pests. To enhance these programs, markers can be used in a mark-release-recapture (MRR) method to track hoverfly feeding and oviposition sites.

Photoswitching Lanthanoid Luminescence with Diazocines.

The unique photophysical properties of the lanthanoids provide the core functionality for various high-tech applications including optoelectronic materials, lasers, anti-counterfeiting dyes or tools for biological sciences. Combination of these luminophores with photoswitches has the potential to make them remote controllable and adds new layers of complexity to their applications. We herein report that such a photomodulation of EuIII, TbIII and YbIII luminescence is possible with the aid of diazocine photoswitches.

Electric-Dipole Coupling Ion-Dipole Engineering Induced Rational Solvation-Desolvation Behavior for Constructing Stable Solid-State Lithium Metal Batteries.

Solid-state polymer electrolytes (SPEs) with high ionic conductivity, a wide voltage window, and an ultra-stability electrolyte/electrode interface are essential for practical applications of solid lithium-metal batteries but particularly challenging. The key to overcoming these long-term obstacles lies in the rational design of the Li+ solvation-desolvation behavior in SPEs. Herein, we propose an electric-dipole coupling ion-dipole strategy to modulate the Li+ solvation structure and enhance Li+ desolvation kinetics.

Asymmetric Structural Design in Cobaloxime-Integrated Covalent Organic Frameworks to Facilitate Photocatalytic Overall Water Splitting​.

Herein, we report a hydrogen-bonded supramolecular hybrid composed of a cobaloxime complex and a covalent organic framework (COF) that achieves photocatalytic overall water splitting. The designed COF features ternary units and dual linkages, which induce asymmetric linkers. Upon photoexcitation, this asymmetry facilitates directed electron transfer and spatially separated redox sites, resulting in a long-lived charge-separated state.

Design and Synthesis Of Heptadecane-Linked 1,2,4-Triazoles As Antimicrobial Agents.

Superbugs, as bacterial resistance species, resist known antibiotics. In this study, twenty novel 1,2,4-triazole-functionalized piperazine-stearic acid derivatives 6a-p were designed, synthesized, and characterized via spectroscopic analyses, evaluating their antimicrobial potential against clinically relevant bacterial and fungal strains. Notably, five derivatives (6a, 6c, 6m, 6n, and 6o) demonstrated superior antimicrobial efficacy compared to gentamicin standard, with compound 6n showing the most potent activity (MIC = 3.

Comparative analysis of mortality patterns and treatment strategies in thalassaemia and sickle cell disease patients: A 12-year study.

This study examined mortality rates among 2475 patients with thalassaemia and sickle cell disease (SCD) per year over 12 years in Greece, recording 335 deaths (27.92/year), with an overall mortality rate of 1.13% per year.

Synthesis and Properties of Cyclic Imide Extended Diazocines: Tweezer-Like, Rigid Photoswitches with Large Switching Amplitudes.

Diazocines are bridged azobenzenes with improved photophysical properties. One of the main features is the fact that, unlike the azobenzenes, the Z form is more stable than the E isomer. Another important property is the more rigid structure (tricyclic), which precisely defines the molecular movement.

Directed Evolution of Fluorescent Genetically Encoded Biosensors: Innovative Approaches for Development and Optimization of Biosensors.

Fluorescent protein-based biosensors are indispensable molecular tools in cell biology and biomedical research, providing non-invasive dynamic measurements of metabolite concentrations and other cellular signals. Traditional methods for developing these biosensors rely on rational design, but directed evolution methods offer a more efficient alternative. This review discusses recent advancements in the development of biosensors using directed evolution, including methods for optimizing domain fusions, sequence optimization, and new screening and selection systems.

Analysis of N,N-dimethyltryptamine (DMT) and its metabolites using LC-MS/MS for forensic purposes.

Ayahuasca contains N,N-dimethyltryptamine (DMT), the primary alkaloid responsible for its psychedelic effects. DMT oxidative deamination yields indole-3-acetic acid (IAA) as the predominant metabolite, while N-oxidation produces N,N-dimethyltryptamine-N-oxide (DMT-NO) as the second most abundant metabolite. An LC-MS/MS method was developed and validated to quantify DMT, IAA, and DMT-NO in human plasma, as well as DMT and DMT-NO in human urine.

Efficient Photosynthesis of Hydrogen Peroxide from Water and Air over Water-Dispersible Anthraquinone-Based Porous Aromatic Frameworks.

Photosynthesis of hydrogen peroxide from earth-abundant water and air over organo-based semiconducting materials is a promising alternative to the traditional anthraquinone (AQ) method. However, the generally hydrophobic nature of organic semiconductors has led to their poor dispersibilities in aqueous systems, which built huge barriers for photon capture and reactant contact in water-based photocatalysis. Aiming at this issue, this study reports the facile synthesis of AQ-based porous aromatic frameworks (AQ-PAFs) by coupling of AQ fragments with thiophene-derived linkers via robust carbon-carbon bonds.