Characterization of the pathogenicity of a isolate from the Mariana Trench.

is an important opportunistic pathogen widely distributed in the environment. In this study, we reported the isolation and characterization of a isolate, MB1, from the Challenger Deep of the Mariana Trench. MB1 is aerobic, motile, and able to form endospores.

Temporal-spatial pattern and driving factors of cultivated land use transition at country level in Shaanxi province, China.

The county-level Cultivated Land Use Form index (CLUF) in Shaanxi province in 2000, 2005, 2010, and 2015 was measured with the entropy method and the linear combination method. Then, the spatial differentiation characteristics and driving mechanism of CLUF were characterized and identified through exploratory spatial data analysis, standard deviation ellipse model, kernel density estimation, multiple linear, and spatial regression analysis. The conclusions drawn from empirical results were as follows.

Characterization of a Deep Sea Isolate: Genomic and Pathogenic Features.

is a group of Gram-positive bacteria belonging to the group and used in some cases as probiotics or biocontrol agents. To our knowledge, from the deep sea (depth >1,000 m) has not been documented. Here, we report the isolation and characterization of a strain, P18, from a deep sea hydrothermal field.

Human adenylate kinase 6 regulates WNK1 (with no lysine kinase-1) phosphorylation states and affects ion homeostasis in NT2 cells.

Adenylate kinase 6 (AK6), a nucleus localized phosphotransferase in mammalians, shows ubiquitously expression and broad substrate activity in different tissues and cell types. Although the function of AK6 has been extensively studied in different cancer cell lines, its role in mammalian germline is still unknown. Here we showed that knockdown of AK6 inhibits cell proliferation and promotes cell apoptosis in human testicular carcinoma (NT2 cells).

Complete mitochondrial genome of the jellyfish, (Goette, 1886) (Cnidaria, Scyphozoa) and the phylogenetic relationship in the related species.

The complete mitochondrial genome sequences of giant jellyfish , a scyphozoan species inhabiting the Bohai Sea water in China, is firstly described and analyzed in this research. The mitogenome is a circular molecule 16,964 bp in length, including 13 protein-coding genes (Cox 1, Cox2, Atp 8, Atp 6, Cox 3, ND2, ND5, ND 6, ND3, ND4L,ND1,ND4, Cob), 2 tRNAs (trnW, trnM), 2 rRNA genes (small subunit RNA and large subunit RNA). The neighbor-joining (NJ) phylogenetic tree in the related species showed that is close to .

Exploring community evolutionary characteristics of microbial populations with supplementation of green tea extracts in microbial fuel cells.

This first-attempt study deciphered combined characteristics of species evolution and bioelectricity generation of microbial community in microbial fuel cells (MFCs) supplemented with green tea (GT) extracts for biomass energy extraction. Prior studies indicated that polyphenols-rich extracts as effective redox mediators (RMs) could exhibit significant electrochemical activities to enhance power generation in MFCs. However, the supplementation of GT extract obtained at room temperature with significant redox capabilities into MFCs unexpectedly exhibited obvious inhibitory effect towards power generation.

Influence of textile dye and decolorized metabolites on microbial fuel cell-assisted bioremediation.

As known, decolorized metabolites (DMs) were capable to act as electron shuttles (ESs) to enhance color removal of textile dye(s); however, optimal manipulation of such advantages to microbial fuel cell (MFC)-assisted dye decolorization for industrial practicability were still remained open to be disclosed. The novelty of this work was to disclose such DMs-supplementing strategies for the most promising reductive decolorization in MFC-assisted bioremediation. Quantitative assessment clearly indicated that MFCs coupled to DMs accumulation was economically-feasible strategy of bioaugmentation and biostimulation.

Deciphering synergistic characteristics of microbial fuel cell-assisted dye decolorization.

This study provided a novel evaluation scheme to quantitatively reveal "synergistic" stimulation of microbial fuel cell (MFC)-assisted dye decolorization for industrial practicability. This work also disclosed why dye decolorization was more electrochemically favorable during simultaneous bioelectricity generation and dye decolorization (SBG&DD). Quantitative assessment upon stimulating effects of different decolorized metabolites on BG and DD alone was also implemented for conclusive remarks.

Exploring redox-mediating characteristics of textile dye-bearing microbial fuel cells: thionin and malachite green.

Prior studies indicated that biodecolorized intermediates of azo dyes could act as electron shuttles to stimulate wastewater decolorization and bioelectricity generation (WD&BG) in microbial fuel cells (MFCs). This study tended to explore whether non-azo textile dyes (i.e.

Deciphering characteristics of bicyclic aromatics--mediators for reductive decolorization and bioelectricity generation.

This first-attempt study quantitatively assessed electron-mediating characteristics of bicyclic aromatics - 1-amino-2-naphthol, 4-amino-1-naphthol (i.e., decolorized intermediates of azo dyes - orange I and II) for color removal and power generation in MFCs.

Sphingosine 1-phosphate antagonizes the effect of all-trans retinoic acid (ATRA) in a human colon cancer cell line by modulation of RARβ expression.

All-trans retinoic acid (ATRA) is a promising therapeutic agent, but exhibits low efficacy against human cancers. We investigated the effect of sphingosine-1-phosphate (S1P) on ATRA activity in human colon cancer HT-29 cells. S1P antagonized ATRA activity on HT-29 cell proliferation and retinoic acid receptor beta (RARβ) expression.

Exploring new strains of dye-decolorizing bacteria.

This study unveiled a new strategy to explore new indigenous strains with excellent decolorization capabilities from freshwaters and seawaters. Two new bacterial decolorizers DX2b and SH7b, which have the capability to decolorize textile dyes, were isolated from Cross-Strait Taiwan and China. According to PCR-augmented 16S rRNA gene analyses for strain identification, >99% of nucleotide sequences in isolated strains were identical to type strains Rahnella aquatilis, Acinetobacter guillouiae, Microvirgula aerodenitrificans, and Pseudomonas sp.

Comparative and functional analysis of testis-specific genes.

The testis is the special male gonad responsible for spermatogenesis and steroidogenesis with complex gene expressions. Characterizing and comparing the testis-specific genes in different species can reveal key genes related to testis specific functions and provide supplementary information for study of human testis function. We screened testis-specific genes from Unigene libraries, total 317, 449 and 147 testis-specific genes were identified for human, mouse and rat, respectively.

Mapping of the human testicular proteome and its relationship with that of the epididymis and spermatozoa.

The testis produces male gametes in the germinal epithelium through the development of spermatogonia and spermatocytes into spermatids and immature spermatozoa with the support of Sertoli cells. The flow of spermatozoa into the epididymis is aided by testicular secretions. In the epididymal lumen, spermatozoa and testicular secretions combine with epididymal secretions that promote sperm maturation and storage.

Exploring characteristics of bioelectricity generation and dye decolorization of mixed and pure bacterial cultures from wine-bearing wastewater treatment.

This study uncovered microbial characteristics of bioelectricity generation and dye decolorization in single-chamber microbial fuel cells (MFCs) using activated sludge for wine-containing wastewater treatment. Phylogenetic tree analysis on 16S rRNA gene fragments indicated that the predominant strains on anodic biofilm in acclimatized MFCs were Gamma-Proteobacteria Aeromonas punctata NIU-P9, Pseudomonas plecoglossicida NIU-Y3, Pseudomonas koreensis NIU-X8, Acinetobacter junii NIU-Y8, Stenotrophomonas maltophila NIU-X2. Our findings showed that the current production capabilities of these pure strains were only ca.

Comparative study on characteristics of azo dye decolorization by indigenous decolorizers.

This study provides a first attempt to explore indigenous strains with excellent decolorization capability from the most biodiverse region in Taiwan for dye-bearing wastewater treatment. Bacterial isolates were obtained via serial selections under selection pressure of the fungicide nystatin and model textile dye(s). According to profiles of protein expression and PCR-augmented 16S rRNA gene analyses for strain identification, >99% of nucleotide sequences in isolated strains were identical to type strains Aeromonas hydrophila, Klebsiella pneumoniae, Enterobacter cancerogenus, Proteus hauseri, Acinetobacter johnsonii.