Engineered collagen-binding bone morphogenetic protein-2 incorporated with platelet-rich plasma accelerates lumbar fusion in aged rats with osteopenia.

In aged individuals, osteopenia is a great concern for achieving solid spinal fusion. Spinal malunion could lead to various implant-related complications and reduce postoperative quality of life. This study aims to investigate the efficacy of collagen-binding bone morphogenetic protein-2 (CBD-BMP-2) on the treatment of lumbar inter-transverse defects and to explore whether platelet-rich plasma could help CBD-BMP-2 to achieve a better outcome in terms of osteogenesis in senile rats with osteopenia.

The Society for Translational Medicine: clinical practice guidelines for mechanical ventilation management for patients undergoing lobectomy.

Patients undergoing lobectomy are at significantly increased risk of lung injury. One-lung ventilation is the most commonly used technique to maintain ventilation and oxygenation during the operation. It is a challenge to choose an appropriate mechanical ventilation strategy to minimize the lung injury and other adverse clinical outcomes.

Synthesis and Evaluation of (18)F-labeled Pyridaben Analogues for Myocardial Perfusion Imaging in Mice, Rats and Chinese mini-swine.

This study reports three novel (18)F-labeled pyridaben analogues for potential myocardial perfusion imaging (MPI). Three precursors and the corresponding nonradioactive compounds were synthesized and characterized. The radiolabeled tracers were obtained by substituting tosyl with (18)F.

Endovascular treatment for ruptured and unruptured vertebral artery dissecting aneurysms: a meta-analysis.

Background: Different endovascular modalities have been applied to the treatment of vertebral artery dissecting aneurysms, the most commonly used being internal trapping and stent-assisted coiling, although the ideal treatment remains controversial.

Objective: To perform a meta-analysis to study clinical outcomes of patients with vertebral artery dissecting aneurysms who were treated with internal trapping or stent-assisted coiling.

Materials And Methods: We conducted a meta-analysis of eight retrospective studies that compared internal trapping with stent-assisted coiling for the treatment of vertebral artery dissecting aneurysms.

Synthesis and bioevaluation of 4-chloro-2-tert-butyl-5-[2-[[1-[2-[(18) F]fluroethyl]-1H-1,2,3-triazol-4-yl]methyl]phenylmethoxy]-3(2H)-pyridazinone as potential myocardial perfusion imaging agent with PET.

This study reports the synthesis and characterization of 4-chloro-2-tert-butyl-5-[2-[[1-[2-[(18) F]fluroethyl]-1H-1,2,3-triazol-4-yl]methyl]phenylmethoxy]-3(2H)-pyridazinone ([(18) F]Fmp2) for myocardial perfusion imaging (MPI). The tosylate precursor and non-radioactive compound [(19) F]Fmp2 were synthesized and characterized by infrared, (1) H-NMR, (13) C-NMR, and mass spectra (MS). The radiotracer [(18) F]Fmp2 was obtained by one-step nucleophilic substitution of tosyl with (18) F, and evaluated as an MPI agent in vitro and in vivo.

Interaction between Neuromelanin and Alpha-Synuclein in Parkinson's Disease.

Parkinson's disease (PD) is a very common neurodegenerative disorder characterized by the accumulation of α-synuclein (α-syn) into Lewy body (LB) inclusions and the loss of neuronmelanin (NM) containing dopamine (DA) neurons in the substantia nigra (SN). Pathological α-syn and NM are two prominent hallmarks in this selective and progressive neurodegenerative disease. Pathological α-syn can induce dopaminergic neuron death by various mechanisms, such as inducing oxidative stress and inhibiting protein degradation systems.

The diagnosis of spinal dural arteriovenous fistulas.

Study Design: Retrospective consecutive case series.

Objective: To review and analyze clinical presentations and radiological imaging of 326 consecutive patients with spinal dural arteriovenous fistula (SDAVF) from 2 institutions.

Summary Of Background Data: The clinical presentations of SDAVF are nonspecific.

Nuclear translocation of alpha-synuclein increases susceptibility of MES23.5 cells to oxidative stress.

α-Synuclein (α-syn) and oxidative stress play pivotal roles in the pathogenesis of Parkinson's disease (PD). However, the mechanisms underlying the interaction between α-syn and oxidative stress remain poorly understood. The present study provides evidence to suggest that the nuclear translocation of α-syn increases death of dopaminergic neurons in response to oxidative stress.

Age-related decline in motor behavior and striatal dopamine transporter in cynomolgus monkeys.

Advanced human aging is associated with progressive declines of motor function and a risk factor for Parkinson's disease, which mainly involves central nigrostriatal dopaminergic system. The present study investigated age-related changes in motor behaviors and alterations of the number of nigrostriatal dopaminergic terminals in non-human primates. A total of 30 cynomolgus monkeys (Macaca fascicularis) of age 3.

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry combined with magnetic beads for detecting serum protein biomarkers in parkinson's disease.

Background: Biomarkers for neurodegenerative diseases are essential to facilitate disease diagnosis. Application of proteomics has greatly hastened the search for novel biomarkers. In this study, new potential biomarkers were discovered, and a diagnostic pattern was established for idiopathic Parkinson's disease (PD) by using proteomic technology.

Impairment and compensation coexist in amnestic MCI default mode network.

Mild cognitive impairment (MCI) is the transitional, heterogeneous continuum from healthy elderly to Alzheimer's disease (AD). Previous studies have shown that brain functional activity in the default mode network (DMN) is impaired in AD patients. However, altering DMN activity patterns in MCI patients remains largely unclear.

Effect of nonlinear three-dimensional optimized reconstruction algorithm filter on image quality and radiation dose: validation on phantoms.

A new technique called the nonlinear three-dimensional optimized reconstruction algorithm filter (3D ORA filter) is currently used to improve CT image quality and reduce radiation dose. This technical note describes the comparison of image noise, slice sensitivity profile (SSP), contrast-to-noise ratio, and modulation transfer function (MTF) on phantom images processed with and without the 3D ORA filter, and the effect of the 3D ORA filter on CT images at a reduced dose. For CT head scans the noise reduction was up to 54% with typical bone reconstruction algorithms (H70) and a 0.

Genetic association between low-density lipoprotein receptor-related protein gene polymorphisms and Alzheimer's disease in Chinese Han population.

Alzheimer's disease (AD) is the most common neurodegenerative disorders in the elderly. Low-density lipoprotein receptor-related protein (LRP), as a receptor of apolipoprotein E (APOE), APP, and alpha2 macroglobulin (alpha2-M), keeps the balance between degeneration and production of beta-amyloid protein (Abeta) clearance. Its gene had been defined as a candidate gene for AD, but the results were not universal.

[Effects of epimedium flavonoids on synapse related protein in brains of dementia transgenic mice].

Objective: To investigate changes of synapse related protein, such as synaptophysin (SYP) and postsynaptic dense material 95 (PSD-95), in brains of 10 months old transgenic mice, and the effects of Epimedium flavonoids (EF) on expression of SYP and PSD-95 in brain of 10 months old of APP transgenic mice.

Methods: The mice of drug treated group were administered intragastrically by EF (at low doses of 0.03 and high dose of 0.

[Dysembryoplastic neuroepithelial tumor: a clinicopathologic and immunohistochemical study].

Objective: To study the clinicopathologic features, immunophenotype and histogenesis of dysembryoplastic neuroepithelial tumor (DNT).

Methods: Fourteen cases of DNT were retrieved from the archival files of the Department. The histopathologic features and immunohistochemical findings were retrospectively studied.

[Aberrant immunophenotypes in 126 patients with acute leukemia].

The existence of leukemia aberrant immunophenotypes (LAIP) has been suggested to be a valuable tool for the detection of minimal residual disease (MRD), as they could distinguish leukemic cells from normal hematopoietic progenitors. This study was purposed to analyze the characteristics of LAIP in acute leukemia and further explore the proportion of different types of LAIP in acute leukemia patients. Flow cytometry (FCM) with four color and CD45/SSC gating were used to detect the antigen expression in samples of bone marrow from 126 patients with acute leukemia.

cDNA cloning, prokaryotic expression and purification of rat alpha-synuclein.

Objective To clone the cDNA of rat alpha-Syn gene, investigate its prokaryotic expression and produce purified recombinant rat alpha-Syn protein. Methods Rat alpha-Syn cDNA was amplified from the rat brain total RNA by RT-PCR and was cloned into pGEX-4T-1, a prokaryotie expressing vector. The recombinant plasmid containing rat alpha-Syn gene was transformed into E.

[Effect of 6-hydroxydopamine on the levels of phosphorylation of signal transduction and transcription factor 1 and 3: experiment with dopaminergic neurons].

Objective: To observe the effects of 6-hydroxydopamine (6-OHDA) on the levels of phosphorylation of signal transducer and activator of transcription (STAT) 1 and STAT3, and the role of STASD in the neurotoxicity of 6-OHDA on dopaminergic neurons so as to help study the treatment of Parkinson's disease.

Methods: Dopaminergic neurons, human neuroblastoma cells of the line SH-SY5Y were cultured and were treated with 6-OHDA of the concentrations of 50, 100, 200, and 400 micromol/L respectively. SH-SY5Y cells not treated with 6-OHDA were used as control group.

Effect of alpha-synuclein on the promoter activity of tyrosine hydroxylase gene.

Objective: To approach the associated mechanism by which alpha-synuclein (alpha-Syn) might regulate the metabolism of dopamine.

Methods: A DNA fragment, located at -495 to +25 of the human tyrosine hydroxylase (TH) gene, was amplified by PCR and inserted into the pGL(3)-Basic luciferase reporter vector. The recombinant plasmid pGL(3)-THprom was transfected into a dopaminergic cell line MES23.

[Correlation of cholesterol 24-hydroxylase and ATP-binding cassette transporter A1 polymorphisms with Alzheimer's disease].

Objective: To investigate the association of the single nucleotide polymorphisms (SNPs) A-->G in the intron 2 of cholesterol 24-hydroxylase (CYP46) gene and G-->A (R219K) in the exon 6 of ATP-binding cassette transporter A1 (ABCA1) gene with sporadic Alzheimer's disease (SAD) in the Han Chinese population.

Methods: Peripheral blood samples were collected from 168 SAD patients, 74 males and 94 females, aged 74 +/- 8 (52 - 95), with an average age at onset of 69 +/- 7 (47 - 86), and sex-, and age-matched 215 healthy persons. PCR-restriction fragment length polymorphism (PCR-RFLP) was used to detect the genotypes.

Altered functional connectivity of primary visual cortex in early blindness.

In early blindness, the primary visual area (PVA) loses the ability to process visual information, and shifts to working on the processing of somatosensory input, auditory input, and some higher-level cognitive functions. It has not yet been investigated whether such functional changes can lead to alterations of the functional connectivity between the PVA and other brain areas in resting state. The purpose of this study is to investigate the differences in the functional connectivity of the PVA between early blind and sighted subjects using resting functional MRI data.

Plasticity of the corticospinal tract in early blindness revealed by quantitative analysis of fractional anisotropy based on diffusion tensor tractography.

Early visual deprivation may induce plastic changes, not only in the visual system, but also in the remaining sensory systems, secondary to altered experience in these spared modalities. Most of previous studies were focused on the plasticity of cortical areas of sensory modalities, but little attention was paid to the plasticity of motor system and white matter fiber tracts. Our purpose is to investigate the plasticity of the corticospinal tract (CST) in early blindness by tract-based quantitative analysis of fractional anisotropy (FA).

[Advancement in the methods for gene dosage analysis].

Gene dosage determination is increasingly important for the study of both genome variation and rearrangement associated with complex diseases. Large genomic duplications and deletions are increasingly found as the causes. Methods such as PCR or sequencing are usually qualitative rather than quantitative.