Establishment of a Raman microsphere-based immunochromatographic method for the combined detection of influenza A and B viruses and SARS-CoV-2 antigen on a single T-line.

A simple and rapid method based on Raman microsphere immunochromatography was developed in this study for the simultaneous detection of influenza A and B viruses and SARS-CoV-2 on a single test T-line. Three types of Raman microspheres with different Raman characteristics were used as the signal sources and were labelled with monoclonal antibodies against FluA, FluB and SARS-CoV-2, respectively. A mixture of antibodies containing anti-FluA monoclonal antibody, anti-FluB monoclonal antibody and anti-SARS-CoV-2 was sprayed on the detection line (T), and goat polyclonal antibody to chicken (IgY) encapsulated on the quality control line (C), for qualitative detection of these three viruses by the double antibody sandwich method.

Predictive machine learning models based on VASARI features for WHO grading, isocitrate dehydrogenase mutation, and 1p19q co-deletion status: a multicenter study.

The objective of our study was to develop predictive models using Visually Accessible Rembrandt Images (VASARI) magnetic resonance imaging (MRI) features combined with machine learning techniques to predict the World Health Organization (WHO) grade, isocitrate dehydrogenase (IDH) mutation status, and 1p19q co-deletion status of high-grade gliomas. To achieve this, we retrospectively included 485 patients with high-grade glioma from the First Affiliated Hospital of Xinjiang Medical University, of which 312 patients were randomly divided into a training set (n=218) and a test set (n=94) in a 7:3 ratio. Twenty-five VASARI MRI features were selected from an initial set of 30, and three machine learning models - Multilayer Perceptron (MP), Bernoulli Naive Bayes (BNB), and Logistic Regression (LR) - were trained using the training set.

[Application of Demirjian and Chaillet method in age estimation of Uyghur and Han children and adolescents in Urumqi].

Purpose: To evaluate the applicability and accuracy of Demirjian and Chaillet method in estimating the actual age of Uygur and Han children and adolescents in Urumqi.

Methods: A total of 1144 orthopantomograms were included in the study, and the seven permanent teeth in the left jaw were divided into different stages according to two dental age estimation methods, and the dental age was converted to tooth age after checking the table and assigning points, and the dental age and its chronological age were compared with t test or rank sum test using SPSS 21.0 software package, and the accuracy of the two methods was evaluated by comparing the mean absolute error of the two methods.

Erratum: Overexpression of ZEB1 and YAP1 is related to poor prognosis in patients with gliomas with different IDH1 status.

[This corrects the article on p. 138 in vol. 16, PMID: 37559682.

Overexpression of ZEB1 and YAP1 is related to poor prognosis in patients with gliomas with different IDH1 status.

Objective: Whether there is a correlation between zinc-finger E-box-binding homolog 1 (ZEB1) and Yes-associated protein 1 (YAP1) with clinical outcome in gliomas remains unclear. Hence, this study aimed to investigate the effects of ZEB1 and YAP1 on the prognosis of human gliomas and its relationship with the isocitrate dehydrogenase 1 (IDH1) gene state.

Methods: Immunohistochemical staining was used to record the expression levels of ZEB1, YAP1, and p-YAP1 in 122 cases of low-grade glioma (LGGs) and 69 cases of glioblastoma (GBMs).

Protective effects of Chinese herbal monomers against ischemia-reperfusion injury.

Objectives: Ischemia-reperfusion injury is a complicated pathologic process that involves multiple factors including oxidative stress, endoplasmic reticulum stress, calcium overload, inflammatory response, disturbances in energy metabolism, apoptosis, and some newly-described forms of programmed cell death (e.g., necroptosis, autophagy, pyroptosis, patanatos, and ferroptosis).

[Mechanism of Cistanches Herba in treatment of cancer-related fatigue based on network pharmacology and experimental verification].

This study aimed to explore the mechanism of Cistanches Herba in the treatment of cancer-induced fatigue(CRF) by network pharmacology combined with in vivo and in vitro experiments to provide a theoretical basis for the clinical medication. The chemical constituents and targets of Cistanches Herba were searched from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP). The targets of CRF were screened out by GeneCards and NCBI.

Liposomes loaded with dual clinical photosensitizers for enhanced photodynamic therapy of cervical cancer.

Photodynamic therapy (PDT) has become a potential anti-cancer strategy owing to its negligible invasiveness, low toxicity, and high selectivity. The photosensitizer (PS) plays an indispensable role in PDT. Herein, a novel type of PS (Ce6-MB@Lips) which can be excited by a near-infrared (NIR) laser was designed and synthesized.

[Influencing factors of nutritional status and change in 50 patients with oral squamous cell carcinoma during treatment].

Purpose: To investigate the changes of nutritional status in patients with oral squamous cell carcinoma(OSCC) and analyze the influencing factors during treatment.

Methods: Anthropometry (weight, BMI, waistline, middle circumference of left and right upper arms) and laboratory index(serum prealbumin, serum albumin, transferrins, 25-hydroxyvitamin D) were measured to represent the nutritional status of 50 patients with OSCC before operation, two days, one month and three months after operation. SPSS 24.

Effect of hemodynamic changes on the risk of intracranial aneurysm rupture: a systematic review and meta-analysis.

Objective: In this study, the hemodynamic parameters of ruptured intracranial aneurysms (IAs) in various studies were summarized and analyzed to provide predictive parameters for IA rupture in clinical work.

Methods: We searched PubMed, Web of science, Embase, and Cochrane databases for articles published before December 2021 to collect data on hemodynamic parameters associated with IA rupture. Differences in wall shear stress (WSS), oscillatory shear index (OSI), and low wall shear stress area (LSA) between ruptured and unruptured IAs in the literature were summarized and analyzed, and the standardized mean difference (SMD) of 95% CI was calculated by Review Manager 5.

What is the Potential Interplay between Microbiome and Tumor Microenvironment in Oral Squamous Cell Carcinomas?

Oral cancer, with an around 50% mortality rate, is one of the most common malignancies world-wide. It is often detected in advanced or terminal stage and has a poor prognosis, although substantial progress in cancer management. Microbiome has become an increasingly recognized factor that may contribute to the cancerous development.

Polymer encapsulated clinical ICG nanoparticles for enhanced photothermal therapy and NIR fluorescence imaging in cervical cancer.

Photothermal therapy (PTT) is a popular tumor therapy method, which is based on efficient photothermal nanoagents (PTNs). Clinical Indocyanine Green (ICG), as a Food and Drug Administration (FDA) approved agent, is an often-used PTN, meanwhile it is also a good near-infrared (NIR) fluorescence contrast agent. However, the further applications of ICG in biomedical fields are limited due to its poor stability.

Predictive power of high-resolution vessel wall magnetic resonance imaging in ischemic stroke.

Background And Objective: Intracranial atherosclerotic disease (ICAD) is a key contributor to ischemic stroke and has a high recurrence rate. This study aimed to investigate the function of high-resolution vessel wall MRI (HR-VW-MRI) and evaluate plaque characteristics in patients with ICAD.

Methods: A consecutive series of patients with ICAD who underwent HR-VW-MRI were enrolled, and imaging measurements were acquired.

[Effect of conservative treatment on jaw symmetry of children with unilateral condylar fracture].

Purpose: To investigate the effect of conservative treatment of unilateral condylar fractures on jaw symmetry in children.

Methods: Twenty-four children with unilateral condylar fractures were divided into control group and experimental group according to the treatment methods, with 12 cases in each group. The control group received surgery, and the experimental group received conservative treatment with intermaxillary traction combined with occlusal pads.

Circ_0016418 promotes melanoma development and glutamine catabolism by regulating the miR-605-5p/GLS axis.

Background: Malignant melanoma is a skin cancer with a high rate of metastasis. Numerous circular RNAs (circRNAs) have been shown to play vital roles in melanoma. This research aimed to investigate the role and molecular basis of circ_0016418 in melanoma progression.

Downregulation of microRNA-519 enhances development of lung cancer by mediating the E2F2/PI3K/AKT axis.

MicroRNA-519 (miR-519) acts as an inhibitor in different kinds of tumors. The current study was set to probe the function of miR-519 in lung cancer and to explore the potential molecular mechanism. The expression difference of miRNAs between lung cancer and paracancerous tissues was analyzed by microarray.

MiR-28-5p promotes human glioblastoma cell growth through inactivation of FOXO1.

Objective: Glioblastomais is one of the main universal, primary brain cancers, in adults, that has an extremely poor clinical prognosis and a median living period of 12-15 months, accounting for nearly 3-4% of all cancer-related deaths. MicroRNAs (miRNAs) play key roles in cancer pathogenesis by binding the specific and complementary sequences of the 3'UTR of target mRNAs to regulate protein synthesis. Therefore, recognizing functional miRNAs and the fundamental molecular mechanisms will offer novel evidences for the progress of targeted malignancy interferences.

Regulation of calcium pump through Notch/Jagged/Hes signaling pathway in canine model of chronic atrial fibrillation.

Objective: Using a canine model of atrial fibrillation (AF) induced by chronic pacing of left atrial fibrillation, the present study aimed to investigate the protein expression and content change of Notch-1 and its downstream target genes including Hes1, Jagged1, and SERCA2a in the atrial myocardium of canines with chronic AF. Furthermore, the correlation between Notch-1/Hes1/Jagged1 and the SERCA2a calcium pump was also analyzed.

Methods: Ten healthy Beagle dogs including both males and females, aged 7-9 years were randomly divided into a sham group (n = 5) and AF group (n = 5).

Associations between single nucleotide polymorphisms in miR-221, self-reported essential hypertension, and interactions between genetic and environmental factors: a multiethnic study in China.

This case-control study explored the relationship between SNPs in miR-221 and self-reported essential hypertension, as well as interactions between genetic and environmental factors, in a multiethnic Chinese cohort. A MassArray analysis was performed to genotype 462 patients with essential hypertension and 442 healthy participants. The association between four SNPs in miR-221 and essential hypertension risk was determined by investigating the differences in allelic and genotypic frequencies between case and control groups using PLINK version 1.

Epigenetic silencing of miR-137 induces resistance to bicalutamide by targeting TRIM24 in prostate cancer cells.

Prostate cancer is an important hormone-dependent cancer affecting men. In the initial stages, prostate cancer is often treated using hormone therapy, including bicalutamide. Despite the initial effectiveness of this therapy, the tumor eventually acquires resistance, resulting in recurrence of castration-resistant prostate cancer (CRPC).

[Clinical characteristics of oral and maxillofacial space infection in patients with different ages].

Purpose: To analyze the general information,clinical symptoms, etiology of infection, and complications of oral and maxillofacial space infection in patients with different ages, in order to provide references for prevention of complications.

Methods: Three hundred and forty-eight patients with oral and maxillofacial space infection treated in the Oncology Department of Oral and Maxillofacial Surgery of the First Affiliated Hospital of Xinjiang Medical University were retrospectively analyzed from March 2007 to Feburary 2017. Statistical analysis was performed with SPSS 20.

[Analysis of related factors affecting treatment results in 126 patients with oral and maxillofacial space infection].

Purpose: This study was aimed to retrospectively investigate the related factors of oral and maxillofacial space infection in 126 patients.

Methods: The clinical data of 126 patients with oral and maxillofacial space infection were collected from the Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Xinjiang Medical University during the period of 2015 to 2017. The clinical features (e.

[Logistic regression analysis of risk factors of temporomandibular disorder in undergraduates of Xinjiang Medical University].

Purpose: To investigate the prevalence of temporomandibular disorders (TMD) in undergraduates of Xinjiang Medical University and analyse its possible risk factors.

Methods: A sample of 700 medical students included 244 males and 456 females was selected from Xinjiang Medical University and underwent examination of temporomandibular joint, questionnaire survey. Their average age was 20.

[Distribution of WNT10A gene rs10177996 polymorphism between Han and Uygur populations in Xinjiang area].

Purpose: To explore the characteristics of distribution of WNT10A gene rs10177996 polymorphism between Han and Uygur populations in Xinjiang area.

Methods: A cross-sectional survey on 154 Han individuals in Urumqi area and 134 Uygur individuals in Kashgar area was performed. Buccal epithelial cells were harvested using Cotton swab scraping, and DNA was extracted by special kit.

LincRNA-p21 sponges miR-18b to promote the progression of diabetic nephropathy.

Little is known about the role of lincRNA-p21 in the development of diabetic nephropathy. The aim of the present study was to investigate the level of lincRNA-p21 in diabetic nephropathy, and explore its underlying mechanism. The current study revealed that down-regulation of lincRNA-p21 could alleviate pathological changes of diabetic nephropathy in mice.