FPBA-modified magnetic nanoparticles combined with Au@AgNPs for label-free SERS detection of foodborne pathogens in milk.

We report a low-cost and highly sensitive label-free SERS biosensor for pathogen detection. Herein, this study prepared 4-formylphenylboric acid (FPBA) functionalized magnetic nanoparticles to adsorb pathogenic bacteria through boric acid affinity principle, and used aptamer modified Au@AgNPs as SERS substrate to specifically combine with pathogenic bacteria to form a sandwich structure. The pathogenic bacteria were detected by portable Raman spectrometer for SERS detection, and the fingerprint signals of pathogenic bacteria were analyzed by principal component analysis (PCA) to achieve the purpose of classification and identification of pathogenic bacteria.

Integration of three non-interfering SERS probes combined with ConA-functionalized magnetic nanoparticles for extraction and detection of multiple foodborne pathogens.

A sandwich-structured SERS biosensor has been constructed for simultaneous detection of multiple pathogenic bacteria, consisting of non-interfering SERS probes for bacterial labeling and ConA-functionalizd magnetic nanoparticles for bacteria extraction. A the preparation method of PP3 SERS probe with high Raman activity is reported for the first time. Since the PP3 SERS probe has a very strong Raman peak at 2081 cm in the "Raman silent region," the mixed SERS probe formed with MP1 and DP2 can meet the needs of multiple foodborne pathogen detection.

Recent advancements in microfluidic chip biosensor detection of foodborne pathogenic bacteria: a review.

Foodborne diseases caused by pathogenic bacteria pose a serious threat to human health. Early and rapid detection of foodborne pathogens is an urgent task for preventing disease outbreaks. Microfluidic devices are simple, automatic, and portable miniaturized systems.