Rho GTPase Activating Protein 24 (ARHGAP24) Silencing Promotes Lung Cancer Cell Migration and Invasion by Activating β-Catenin Signaling.

BACKGROUND Rho GTPase activating protein (RhoGAPs) is an important negative regulator of the Rho signaling pathway that is involved in tumorigenesis in liver, colon, and renal cancer. However, the mechanism by which Rho GTPase activating protein 24 (ARHGAP24) regulates cell invasion and migration of lung cancer has not been fully explained. MATERIAL AND METHODS In this study, ARHGAP24 expression in lung cancer tissues and cell lines was measured by immunohistochemical and Western blot analysis.

A Descriptive Qualitative Study of Home Care Experiences in Parents of Children with Tracheostomies.

Purpose: The number of pediatric patients who require a long-term tracheal tube at home is gradually increasing. Studies have demonstrated that the parents of these children report high levels of stress, anxiety and other negative emotions as early as shortly after discharge from the hospital. The purpose of this study is to describe the home care experiences of parents of children with tracheostomies during the transition from hospital to home in China to more effectively address their needs.

Upregulation of miR-181a impairs lipid metabolism by targeting PPARα expression in nonalcoholic fatty liver disease.

Recent studies have reported elevated expression of miR-181a in patients with non-alcoholic fatty liver disease (NAFLD), suggesting that it may play an important role in liver lipid metabolism and insulin resistance. We aimed to investigate the effect of miR-181a in lipid metabolism and find new treatments for NAFLD. The expression level of miR-181a in NAFLD patient serum and a palmitic acid (PA)-induced NAFLD cell model was examined by Q-PCR.

Multiple integrin ligands provide a highly adhesive and osteoinductive surface that improves selective cell retention technology.

Among various bone tissue engineering strategies, selective cell retention (SCR) technology has been used as a practical clinical method for bone graft manufacturing in real time. The more mesenchymal stem cells (MSCs) are retained, the better the osteoinductive microenvironment provided by the scaffold, which in turn promotes the osteogenesis of the SCR-fabricated bone grafts. Integrin receptors are crucial to cell-matrix adhesion and signal transduction.

MicroRNA-101 protects bladder of BOO from hypoxia-induced fibrosis by attenuating TGF-β-smad2/3 signaling.

Bladder outlet obstruction is a common disease, which always evokes urinary bladder wall remodeling significantly. It has been suggested that bladder outlet obstruction can make the bladder progression from inflammation to fibrosis, and hypoxia may play a vital role. It has been found the expression of microRNA-101 varied in bladder after BOO.

First identification of nonsense mutations in autosomal dominant focal segmental glomerulosclerosis.

Recently, a novel heterozygous missense mutation c.T1421G (p. L474R) in the gene encoding podocalyxin was identified in an autosomal dominant focal segmental glomerulosclerosis (AD-FSGS) pedigree.

Imaging-Related Risk Factors for Bleeding Complications of US-Guided Native Renal Biopsy: A Propensity Score Matching Analysis.

Purpose: To evaluate imaging-related hemorrhagic risk factors for ultrasound (US)-guided native kidney biopsy.

Materials And Methods: A retrospective review was conducted of adult patients who underwent US-guided native kidney biopsy at a single center between January 2006 and March 2016 and identified 37 of 551 patients (6.72%) with postbiopsy bleeding complications, including 11 major complications (2.

Early life exposure to famine and reproductive aging among Chinese women.

Objective: To assess the effect of early life exposure to famine, as endured during 1959 to 1961 in China, on reproductive aging in adult women.

Methods: Between 2011 and 2012, 2,868 women born around the Chinese famine period (1956-1964) were enrolled in this study from three communities in China. Age at natural menopause was obtained retrospectively from a structured questionnaire.

Different distributions of interstitial cells of Cajal and platelet-derived growth factor receptor-α positive cells in colonic smooth muscle cell/interstitial cell of Cajal/platelet-derived growth factor receptor-α positive cell syncytium in mice.

Aim: To investigate the distribution and function of interstitial cells of Cajal (ICCs) and platelet-derived growth factor receptor-α positive (PDGFRα) cells in the proximal and distal colon.

Methods: The comparison of colonic transit in the proximal and distal ends was performed by colonic migrating motor complexes (CMMCs). The tension of the colonic smooth muscle was examined by smooth muscle spontaneous contractile experiments with both ends of the smooth muscle strip tied with a silk thread.

Identification of 8 novel gene variants in primary hyperoxaluria in 21 Chinese children with urinary stones.

Purpose: We analyzed primary hyperoxaluria (PH) genotype and phenotype in Chinese children. Vitamin B response in the patients with genetically confirmed PH1 was also studied.

Methods: We, respectively, analyzed 80 children with urinary stones.

Peripherally inserted central catheter-related complications in infants with intestinal failure.

Background And Objectives: For delivery of parenteral nutrition (PN), long-term central access is often required in infants with intestinal failure (IF). Compared to central venous catheters (CVCs), peripherally inserted central catheters (PICCs) are less invasive, as they are smaller, and they can even be placed without general anesthesia. In this study, we report the complications of long-term use of PICCs, and compare our results with previously published research.

Efficiency of maternal-fetal transfer of perfluoroalkyl and polyfluoroalkyl substances.

Perfluoroalkyl and polyfluoroalkyl substances (PFASs) can be transferred from a mother to her fetus during pregnancy and adversely affect fetal development. However, the efficiency and influencing factors of PFASs maternal-fetal transfer remain unclear. We measured the levels of six perfluoroalkylcarboxylates, three perfluoroalkylsulfonates, and one sulfonamide in 369 pairs of maternal and umbilical cord serum and examined the transplacental transfer efficiency (TTE) of PFASs by the functional group and carbon chain length in a prospective birth cohort in Shandong, China.

Spectrum of Variants in 389 Chinese Probands With Familial Exudative Vitreoretinopathy.

Purpose: To identify potentially pathogenic variants (PPVs) in Chinese familial exudative vitreoretinopathy (FEVR) patients in FZD4, LRP5, NDP, TSPAN12, ZNF408, and KIF11 genes.

Methods: Blood samples were collected from probands and their parent(s). Genomic DNA was analyzed by next-generation sequencing, and the sequence of selected variants were validated by Sanger sequencing.

Biochanin A Induces S Phase Arrest and Apoptosis in Lung Cancer Cells.

Lung cancer is among the most common malignancies with a poor 5-year survival rate reaching only 16%. Thus, new effective treatment modalities and drugs are urgently needed for the treatment of this malignancy. In this study, we conducted the first investigation of the effects of Biochanin A on lung cancer and revealed the mechanisms underlying its potential anticancer effects.

Timing for Ponseti clubfoot management: does the age matter? 90 children (131 feet) with a mean follow-up of 5 years.

Background and purpose - There are still controversies as to the age for beginning treatment with the Ponseti method. We evaluated the clinical outcome with different age at onset of Ponseti management for clubfoot. Patients and methods - 90 included children were divided into 3 groups in terms of age at start of treatment.

LncRNA-CIR promotes articular cartilage degeneration in osteoarthritis by regulating autophagy.

Osteoarthritis (OA) is a common joint disease that is regarded as a local inflammatory response caused by joint instability and accompanied by the progressive degeneration of articular cartilage. However, the molecular mechanisms involved in the maintenance of articular cartilage remain a subject of debate and research. This study aims to analyze the roles of long noncoding RNA (lncRNA)CIR and autophagy in cartilages and determine their overall contribution to the degradation of extracellular matrix.

Monitoring the Progression of Chronic Liver Damage in Rats Using [F]PBR06.

Purpose: Accurate and rapid assessment of liver condition is the key to therapy for hepatitis patients. This study aim is to evaluate the peripheral benzodiazepine receptor (PBR) radioligand [F]N-fluoroacetyl-N-(2,5-dimethoxybenzyl)-2-phenoxyaniline ([F]PBR06) as a positron emission tomography (PET) imaging tracer of chronic liver damage in a rat model.

Procedures: A rat model of liver damage was made by bile duct ligation (BDL), which initiates a complex cascade of pathological events that leads to cholestasis and inflammation, eventually resulting in a severe fibrotic and severe hepatocyte injury.

Correction of Deep Overbite by Using a Modified Nance Appliance in an Adult Class II Division 2 Patient with Dehiscence Defect.

A modified Nance Appliance (MNA) is introduced as a treatment option for an adult class II division 2 malocclusion (CII/2) patient with deep overbite and dehiscence on the facial root surface of retroclined upper incisors through the cone-beam computed tomography (CBCT). Indications for this modified MNA as well as a brief description of fabrication procedure and biomechanical analysis of the treatment effects are shown in detail. Root control and absolute intrusion without enlarging the bony defect were achieved.

PAK5 Induces EMT and Promotes Cell Migration and Invasion by Activating the PI3K/AKT Pathway in Ovarian Cancer.

Ovarian cancer is the most lethal gynecologic cancer and currently ranks fifth in causing cancer-related deaths among women. P21-activated kinase 5 (PAK5) is a newly identified protein that has been indicated to have oncogenic potential. The present study investigated the expression level of PAK5 in clinical ovarian cancer and the functional roles of PAK5 in ovarian cancer progression.

Braided bioresorbable cardiovascular stents mechanically reinforced by axial runners.

Polymeric bioresorbable stents (BRSs) can eliminate the long-term stent restenosis by degrading after vascular remolding and have been recommended for the congenital heart disease treatment. However, the mechanical weakness remains one of main inferiorities of their applications. So, the aim of this study was to develop mechanically reinforced bioresorbable stents (MRBSs) based on poly(p-dioxanone) (PPDO) monofilaments and braiding technology.

Nocturnal enuresis in children from Santo Domingo, Dominican Republic: a questionnaire study of prevalence and risk factors.

Objective: To determine the prevalence of paediatric nocturnal enuresis in Santo Domingo, Dominican Republic.

Design: A cross-sectional study was performed using a pretested questionnaire.

Setting: Three different schools in Santo Domingo, Dominican Republic.

Glycoprotein nmb Is Exposed on the Surface of Dormant Breast Cancer Cells and Induces Stem Cell-like Properties.

Glycoprotein nmb (GPNMB) is a type I transmembrane protein that contributes to the initiation and malignant progression of breast cancer through induction of epithelial-mesenchymal transition (EMT). Although it is known that EMT is associated with not only cancer invasion but also acquisition of cancer stem cell (CSC) properties, the function of GPNMB in this acquisition of CSC properties has yet to be elucidated. To address this issue, we utilized a three-dimensional (3D) sphere culture method to examine the correlation between GPNMB and CSC properties in breast cancer cells.

Lack of Dietary Diversity Contributes to the Gaps in Micronutrient Status and Physical Development between Urban and Rural Infants.

Background: Although the prevalence of malnutrition among Chinese infants has decreased, micronutrient deficiency is still common. This study aimed to describe and compare the status of micronutrient deficiency and its association with dietary variety and socio-demographic features among infants from urban and rural China.

Methods: A cross-sectional study was performed on 1200 children aged 18-month-old from rural villages in Yunnan and an urban city in Shanghai.

Global Perspectives on Nonalcoholic Fatty Liver Disease and Nonalcoholic Steatohepatitis.

Over the past 2 decades, nonalcoholic fatty liver disease (NAFLD) has grown from a relatively unknown disease to the most common cause of chronic liver disease in the world. In fact, 25% of the world's population is currently thought to have NAFLD. Nonalcoholic steatohepatitis (NASH) is the subtype of NAFLD that can progress to cirrhosis, hepatocellular carcinoma (HCC), and death.