Cold atmospheric plasma potentiates ferroptosis via EGFR(Y1068)-mediated dual axes on GPX4 among triple negative breast cancer cells.

Cold atmospheric plasma (CAP) has been proposed as an emerging onco-therapeutics that can specifically kill cancer cells without harming healthy cells. Here we explore its potency in triggering ferroptosis in transformed cells using triple negative breast cancer as the disease model. Through the whole transcriptome sequencing, mass spectrometry analysis, point mutation, and a series of and molecular assays, we identified two signaling axes centered at EGFR(Y1068), i.

Functionalized Periosteum-Derived Microsphere-Hydrogel with Sequential Release of E7 Short Peptide/miR217 for Large Bone Defect Repairing.

Large bone defects are still a persistent challenge in orthopedics. The availability limitations and associated complications of autologous and allogeneic bone have prompted an increasing reliance on tissue engineering and regenerative medicine. In this study, we developed an injectable scaffold combining an acellular extracellular periosteal matrix hydrogel with poly(d,l-lactate--glycol-acetate) microspheres loaded with the E7 peptide and miR217 (miR217/E7@MP-GEL).

Interaction study of the effects of environmental exposure and gene polymorphisms of inflammatory and immune-active factors on chronic obstructive pulmonary disease.

Background: Chronic obstructive pulmonary disease (COPD) is a heterogeneous disease, influenced by both environmental and genetic factors. Single nucleotide polymorphism (SNP) in the human genome may influence the risk of developing COPD and the response to treatment. We assessed the effects of gene polymorphism of inflammatory and immune-active factors and gene-environment interaction on risk of COPD in middle-aged and older Chinese individuals.

Exploring artificial intelligence for differentiating early syphilis from other skin lesions: a pilot study.

Background: Early diagnosis of syphilis is vital for its effective control. This study aimed to develop an Artificial Intelligence (AI) diagnostic model based on radiomics technology to distinguish early syphilis from other clinical skin lesions.

Methods: The study collected 260 images of skin lesions caused by various skin infections, including 115 syphilis and 145 other infection types.

A multi-kinase inhibitor screen identifies inhibitors preserving stem-cell-like chimeric antigen receptor T cells.

Chimeric antigen receptor T cells (CAR T cells) with T stem (T) cell-like phenotypic characteristics promote sustained antitumor effects. We performed an unbiased and automated high-throughput screen of a kinase-focused compound set to identify kinase inhibitors (KIs) that preserve human T cell-like CAR T cells. We identified three KIs, UNC10225387B, UNC10225263A and UNC10112761A, that combined in vitro increased the frequency of CD45RACCR7TCF1 T cell-like CAR T cells from both healthy donors and patients with cancer.

Phosphorylation of endothelial histone H3.3 serine 31 by PKN1 links flow-induced signaling to proatherogenic gene expression.

Atherosclerotic lesions develop preferentially in arterial regions exposed to disturbed blood flow, where endothelial cells acquire an inflammatory phenotype. How disturbed flow induces endothelial cell inflammation is incompletely understood. Here we show that histone H3.

The heart rate on admission was associated with the mortality in elderly patients with hip fractures: a retrospective cohort study from a trauma centre in northwestern China.

Objectives: To evaluate the association between heart rate on admission and mortality in elderly patients with hip fractures.

Design: A retrospective cohort study.

Setting: At a trauma centre in northwestern China.

GSE: A global-local storage enhanced video object recognition model.

The presence of substantial similarities and redundant information within video data limits the performance of video object recognition models. To address this issue, a Global-Local Storage Enhanced video object recognition model (GSE) is proposed in this paper. Firstly, the model incorporates a two-stage dynamic multi-frame aggregation module to aggregate shallow frame features.

The Effects of Resonance Frequency Breathing on Cardiovascular System and Brain-Cardiopulmonary Interactions.

Resonance frequency (RF) is characterized as the specific frequency at which a system, equipped with delayed self-correction or negative feedback mechanisms, exhibits maximal amplitude oscillations in response to an external stimulus of a particular frequency. Emerging evidence suggests that the cardiovascular system has an inherent RF, and that breathing at this frequency can markedly enhance health and cardiovascular function. However, the efficacy of resonance frequency breathing (RFB) and the specific responses of the cardiovascular, respiratory, and central nervous systems during RFB remain unclear.

Single-center experience of venetoclax combined with azacitidine in young patients with newly diagnosed acute myeloid leukemia.

Background: Medical resources, especially blood products, were in short supply during the COVID-19. Less intensive therapy with hypomethylating agents/venetoclax (VEN) seems an effective treatment option for patients with acute myeloid leukemia (AML).

Objectives: To retrospectively analyze the efficacy and safety of VEN combined with azacitidine (AZA) in young adult patients with newly diagnosed (ND) AML.

Mitochondrial dysfunction in pancreatic acinar cells: mechanisms and therapeutic strategies in acute pancreatitis.

Acute pancreatitis (AP) is an inflammatory disease of the pancreas and a complex process involving multiple factors, with mitochondrial damage playing a crucial role. Mitochondrial dysfunction is now considered a key driver in the development of AP. This dysfunction often presents as increased oxidative stress, altered membrane potential and permeability, and mitochondrial DNA damage and mutations.

The benefits of contrast-enhanced ultrasound in the differential diagnosis of suspicious breast lesions.

Background: Contrast-enhanced ultrasound (CEUS) shows potential for the differential diagnosis of breast lesions in general, but its effectiveness remains unclear for the differential diagnosis of lesions highly suspicious for breast cancers.

Objective: This study aimed to evaluate the diagnostic value of CEUS in differentiating pathological subtypes of suspicious breast lesions defined as category 4 of US-BI-RADS.

Methods: The dataset of 150 breast lesions was prospectively collected from 150 patients who underwent routine ultrasound and CEUS examination and were highly suspected of having breast cancers.

Ultra-small quercetin-based nanotherapeutics ameliorate acute liver failure by combatting inflammation/cellular senescence cycle.

Acute liver failure (ALF) is marked by a substantial generation of reactive oxygen species (ROS), which can induce both cellular senescence and a pronounced inflammatory response. Senescent cells secrete factors collectively termed the senescence-associated secretory phenotype (SASP), which exacerbate inflammation, while inflammation can reciprocally promote cellular senescence. Quercetin (Que), recognized for its ROS-scavenging capabilities, holds the potential for anti-inflammatory and anti-senescent effects.

The Investigation of the Relationship Between Individual Pain Perception, Brain Electrical Activity, and Facial Expression Based on Combined EEG and Facial EMG Analysis.

Purpose: Pain is a multidimensional, unpleasant emotional and sensory experience, and accurately assessing its intensity is crucial for effective management. However, individuals with cognitive impairments or language deficits may struggle to accurately report their pain. EEG provides insight into the neurological aspects of pain, while facial EMG captures the sensory and peripheral muscle responses.

Metagenomic next-generation sequencing of cerebrospinal fluid: a diagnostic approach for varicella zoster virus-related encephalitis.

Purpose: Varicella zoster virus-related encephalitis (VZV-RE) is a rare and often misdiagnosed condition caused by an infection with the VZV. It leads to meningitis or encephalitis, with patients frequently experiencing poor prognosis. In this study, we used metagenomic next-generation sequencing (mNGS) to rapidly and accurately detect and identify the VZV pathogen directly from cerebrospinal fluid (CSF) samples, aiming to achieve a definitive diagnosis for encephalitis patients.

Li+ Quasi-Grotthuss Topochemistry Transport Enables Direct Regeneration of Spent Lithium-Ion Battery Cathodes.

Direct regeneration of spent lithium-ion batteries offers economic benefits and a reduced CO2 footprint. Surface prelithiation, particularly through the molten salt method, is critical in enhancing spent cathode repair during high-temperature annealing. However, the sluggish Li+ transport kinetics, which relies on thermally driven processes in the traditional molten salt methods, limit the prelithiation efficiency and regeneration of spent cathodes.

Remote carbon monoxide spillover improves tandem urea electrosynthesis.

Electrocatalytic urea synthesis from carbon dioxide (CO2) and nitrate (NO3-) offers a promising alternative to traditional industrial methods. However, current catalysts face limitations in the supplies of CO* and Nrelated* intermediates, and their coupling, resulting in unsatisfactory urea production efficiency and energy consumption. To overcome these challenges, we carried out tandem electrosynthesis approach using ruthenium dioxide-supported palladium-gold alloys (Pd2Au1/RuO2).

Reconstructing Waddington Landscape from Cell Migration and Proliferation.

The Waddington landscape was initially proposed to depict cell differentiation, and has been extended to explain phenomena such as reprogramming. The landscape serves as a concrete representation of cellular differentiation potential, yet the precise representation of this potential remains an unsolved problem, posing significant challenges to reconstructing the Waddington landscape. The characterization of cellular differentiation potential relies on transcriptomic signatures of known markers typically.

Effectiveness of virtual laboratory in engineering education: A meta-analysis.

With the development of network and simulation technology, virtual laboratories have been widely popularized in engineering education. However, few studies have systematically analyzed and summarized the impact of virtual labs on the effectiveness of engineering education. This study aims to conduct a meta-analysis of published data on the impact of virtual laboratories on engineering students' performance.

Two-dimensional anion-rich NaCl crystal under ambient conditions.

The two-dimensional (2D) "sandwich" structure composed of a cation plane located between two anion planes, such as anion-rich CrI, VS, VSe, and MnSe, possesses exotic magnetic and electronic structural properties and is expected to be a typical base for next-generation microelectronic, magnetic, and spintronic devices. However, only a few 2D anion-rich "sandwich" materials have been experimentally discovered and fabricated, as they are vastly limited by their conventional stoichiometric ratios and structural stability under ambient conditions. Here, we report a 2D anion-rich NaCl crystal with sandwiched structure confined within graphene oxide membranes with positive surface potential.

Supercarbon assembly inspired two-dimensional hourglass fermion.

By using a tight-binding model, first-principles calculations, and ab initio molecular dynamics simulations, we theoretically demonstrate that the C76-Td-assembled two-dimensional (2D) honeycomb lattice is stable at room temperature and is resistant to mechanical deformation. We disclose that each C76-Td mimics a single carbon atom (geometrically and electronically); hence, it plays the role of one supercarbon. This inspires that the 2D material exhibits an exotic hourglass-like fermion at the Fermi level.

The Correlation Between Fecal Amino Acids, Colonic Mucosal Taste Receptors, and Clinical Features and Indicators of Ulcerative Colitis: A Multicenter Exploratory Study.

Background: Patients with ulcerative colitis (UC) exhibit abnormal amino acid (AA) metabolism. Taste receptors play a crucial role in the detection of intestinal AAs. Nevertheless, it remains unclear whether UC patients exhibit abnormal expression of these receptors in the colon.

The association of serum total bile acids and deep venous thrombosis.

Background And Aim: Bile acid metabolism plays a crucial role in maintaining the delicate balance between coagulation and anticoagulation processes. However, there is a paucity of research exploring the relationship between serum total bile acids (TBA) levels and the risk of deep venous thrombosis (DVT) in Chinese individuals. The primary objective of this study is to investigate the association between TBA levels and DVT occurrence in this population.

A new health literacy scale for staff in preschool childcare institution: development and preliminary validation.

Background: The health literacy of staff in preschool childcare institution is an important issue to consider in providing healthcare for children aged 3-6 years, which could contribute to reducing incidence of diseases and accidental injuries as well as maintaining children's good health. Seldom instruments have been designed to measure health literacy across this group. This research aims to develop a health literacy scale for staff in preschool childcare institutions and validate its psychometric properties.

Polymerase delta-interacting protein 2 mediates brain vascular permeability by regulating ROS-mediated ZO-1 phosphorylation and localization at the interendothelial border.

Background: Polymerase delta-interacting protein 2 (Poldip2) is a novel regulator of vascular permeability that has been shown to be involved in aggravating blood-brain barrier (BBB) disruption following stroke; however, the underlying mechanisms are unknown. While endothelial tight junctions (TJ) are critical mediators of BBB permeability, the effect of Poldip2 on TJ function has not been elucidated yet. Here, we aim to define the mechanism by which Poldip2 mediates BBB disruption, specifically focusing on phosphorylation and stabilization of the TJ integral protein ZO-1.