[Clinical study of Vitallium removable partial denture with dual major connector in Kennedy â… and â…¡ dentition defect].

Purpose: To study the clinical effect of Vitallium removable partial denture (RPD) with dual major connector (DMC) in rehabilitation of Kennedy â…  and â…¡ dentition defect.

Methods: A total of 30 patients with Kennedy â…  and â…¡ dentition defect fitting to the inclusion criteria were selected and restored with Vitallium RPD with DMC. Patients were re-called at the 6th and 12th month for clinical follow-up examination of gingival index (GI), abutment teeth mobility (TM), X-ray imaging, probing depth (PD) and satisfaction surveys.

Contribution of Mitophagy to Cell-Mediated Mineralization: Revisiting a 50-Year-Old Conundrum.

Biomineralization in vertebrates is initiated via amorphous calcium phosphate (ACP) precursors. These precursors infiltrate the extracellular collagen matrix where they undergo phase transformation into intrafibrillar carbonated apatite. Although it is well established that ACP precursors are released from intracellular vesicles through exocytosis, an unsolved enigma in this cell-mediated mineralization process is how ACP precursors, initially produced in the mitochondria, are translocated to the intracellular vesicles.

MicroRNA-1292-5p inhibits cell growth, migration and invasion of gastric carcinoma by targeting DEK.

Gastric cancer ranks as the third most lethal cancer worldwide. Although many efforts have been made to identify novel markers for early diagnosis and effective drugs for the treatment of gastric cancer, the outcome is still poor due to delayed diagnosis and lack of therapeutic options. MicroRNAs (miRNAs) play crucial roles during tumorigenesis, and several miRNAs were found to be critical for gastric cancer development, offering promise as therapeutic targets.

[Effect of estrogen on genioglossus and hypoglossal nucleus of female rats].

Puepose: To study the hypoglossal nucleus spontaneous discharge and genioglossus function of SD rats with different estrogen levels, and explore the possible central pathways of estrogen protecting upper airway stability.

Methods: Thirty SD rats were randomly divided into 3 groups: sham operation group (Sham), ovariectomized group (OVX) and ovariectomized + estrogen covering group (OVX+E2). The hypoglossal nucleus spontaneous discharge frequency and maximum amplitude of spontaneous discharge were measured with extracellular recording techniqueï¼›BL-420 biological was used to detect the genioglossus EMG and contraction.

[Precision validation of 3-D reconstruction of the craniofacial hard tissues based on 2-D digital radiograph].

Purpose: In this study, we validated the precision of 3-D craniofacial reconstruction of hard tissues based on 2-D digital cephalometric radiograph, in order to increase the level of orthognathic diagnosis and treatment, and promote communication between doctors and patients.

Methods: Deformation modeling based on 2-D digital radiograph and CT modeling was performed in 7 volunteers. 64 items were measured which described craniofacial hard tissue structure, intuitive overlapping and objective analysis were conducted.

[Effect of topical iron, fluoride application on eroded enamel in vitro].

Purpose: To evaluate the effect of supplementation containing iron and/or fluoride on eroded enamel in vitro.

Methods: Eighty enamel specimens collected in orthodontic treatments were randomly allocated into 4 groups of 20 samples each: C (control,deionized water); Fe(2+)(15 mmol/L FeSO(4)); F(-)(1.23% NaF) and Fe(2+)+ F(-)(15 mmol/L FeSO(4) and 1.

The role of JAK/STAT3 signaling pathway on apoptosis of lung adenocarcinoma cell line PC-9 induced by icotinib.

Objective: The aim of this study is to estimate the role of JAK/STAT3 signaling pathway on apoptosis of lung adenocarcinoma induced by icotinib.

Methods: EGFR mutation was detected in lung adenocarcinoma cell line PC-9 by ARMS assay; The inhibitory rates of cell proliferation of PC-9 cells which were exposed to different concentrations of icotinib (0~100 μMol/L) for different time (24~72 h) respectively were evaluated by MTT assay; Apoptosis of PC-9 cells exposed to different concentrations of icotinib (0, 0.1, 1 and 10 μMol/L) for 48 h were evaluated by TUNEL assay; JAK2, STAT3, Bcl-2, Bax mRNA expressions were evaluated by Real-time PCR assay; The protein levels of P-STAT3 and IL-6 were evaluated by Western-blot assay.

Downregulation of the long non-coding RNA TUSC7 promotes NSCLC cell proliferation and correlates with poor prognosis.

Background: Emerging evidence indicated that dysregulated long non-coding RNAs (lncRNAs) was implicated in the tumorigenesis and progression. LncRNA TUSC7 was involved in various malignancies. However, the role of TUSC7 in human non-small-cell lung cancer (NSCLC) remains unclear.

[Study on enhancement effect and mechanism of the acid resistance of enamel by Fe2+ and F].

Purpose: The phase of new substances and the effect on enamel lattice was analysed after ferrous sulfate ,sodium fluoride treatment through X-ray diffraction, in order to explore the mechanism of Fe2+ and F- reinforced enamel acid resistance.

Methods: Fragments of enamel obtained from fifty molars were ground into powder and randomly divided into four groups: C (control,deionized water); Fe2+ (15 mmol/L FeSO4); F- (1.23% NaF) and Fe2++F- (15 mmol/L FeSO4 and 1.

Effect of bone mesenchymal stem cells transplantation on the micro-environment of early osteonecrosis of the femoral head.

Autologous implantation of bone mesenchymal stem cells (BMSCs) has achieved promising clinical efficacy for the treatment of early-stage osteonecrosis of the femoral head (ONFH). However, the underlying mechanisms are not completely elucidated. Here, we investigated the effect of BMSCs on the early ONFH in vitro and in vivo.

Vitamin C inhibit the proliferation, migration and epithelial-mesenchymal-transition of lens epithelial cells by destabilizing HIF-1α.

Posterior capsular opacification (PCO), the main complication of cataract surgery, is mainly caused by the proliferation, migration, and epithelial-mesenchymal transition (EMT) of the residual lens epithelial cells (LECs).Vitamin C was reported to reduce the risk of forming a cataract. However, there has been no study showing the association between vitamin C and PCO.

rhEPO affects apoptosis in hippocampus of aging rats by upregulating SIRT1.

The aim of this study was to elucidate the signaling pathway involved in the anti-aging effect of erythropoietin (EPO) and to clarify whether recombinant human EPO (rhEPO) affects apoptosis in the aging rat hippocampus by upregulating Sirtuin 1 (SIRT1). In this study, a rat model of aging was established using D-galactose. Behavioral changes were monitored by the Morris water maze test.

Current evidence on the relationship between two common polymorphisms in NPAS2 gene and cancer risk.

The relationship between neuronal PAS domain protein 2 (NPAS2) gene polymorphisms and cancer risk has been widely investigated. However, the results are conflicting. We performed this meta-analysis to derive a more precise estimation on the relationship.

Association of genetic polymorphisms in HTR3A and HTR3E with diarrhea predominant irritable bowel syndrome.

Objective: The present study aims to investigate the relationship between genetic polymorphisms in HTR3A and HTR3E and diarrhea predominant irritable bowel syndrome (D-IBS) in a Chinese population.

Methods: We enrolled 500 D-IBS patients and 500 age- and sex-matched healthy control subjects to detect the genotypes in HTR3A and HTR3B gene by using of PCR-RFLP method.

Results: There were significant difference between the D-IBS patients and the health control subjects in the distribution of genotype and allele of rs1062613 in HTR3A gene.

Effects of PSCA rs2294008 (C/T) and c-MYC rs9642880 (G/T) polymorphisms on bladder cancer: evidence from a meta-analysis.

Previous studies have investigated the associations between the two polymorphisms (prostate stem cell antigen (PSCA) rs2294008 C/T and c-MYC rs9642880 G/T) and bladder cancer (BC) risk. However, the results are inconsistent. We therefore carried out a meta-analysis to estimate the relationship between PSCA/c-MYC polymorphisms and BC risk.

[Expression of TRPV1 and CGRP in rat trigeminal ganglion during orthodontic tooth movement].

Purpose: Sprague-Dawley rat models of tooth movement were established to investigate the expression of transient receptor potential vanilloid 1 (TRPV1) and calcitonin gene related peptide (CGRP) in rat trigeminal ganglion during orthodontic tooth movement, and to explore the roles of TRPV1 and CGRP in orthodontic pain.

Methods: Sixty-six Sprague-Dawley rats were randomly divided into control group (n=6), sham operation group (n=6), and experimental group (n=54). Tooth movement models were established, orthodontic force (50 g) was applied on the maxillary first molar in the rats of experimental group, and then the trigeminal ganglia were collected at 4, 8 h, 1 d (3 subgroups were set up according to the force: 1 d-30 g, 1 d-50 g, 1 d-80 g), 3, 5, 7, 14 d after tooth movement.

Correlation of c-fos protein expression with neuropeptide content in the lung of bronchial asthmatic rat.

Objective: To investigate and analyze the correlation between the c-fos protein expression and neuropeptide content in the lung of bronchial asthmatic rats.

Methods: Thirty-two (32) SD rats were randomly allocated into 4 groups of the normal control, the non-acute asthma, the acute asthma and the dexamethasone intervention. Immunohistochemistry was performed for histological observation, and substance P (SP) and vasoactive intestinal peptide (VIP) concentrations in the bronchoalveolar lavage fluid were measured by enzyme-linked immunosorbent assay (ELISA).

Serum cystatin C as a potential biomarker for the evaluation COPD.

Background: Imbalance between protease-antiprotease plays an important role in the pathogenesis of chronic obstructive pulmonary disease (COPD). Cystatin C in circulating blood is a cysteine protease inhibitor and contributes to elastolysis and tissue destruction.

Objectives: The aims of the present study were to investigate whether cystatin C was a promising biomarker for the evaluation and follow-up of patients with COPD.

Current evidence on the relationship between rs1256049 polymorphism in estrogen receptor-β gene and cancer risk.

Previous studies have suggested that estrogen receptor-β (ESR2) rs1256049 polymorphism is associated with the susceptibility of cancer. However, the results are inconsistent. We performed a meta-analysis to evaluate the association between the rs1256049 polymorphism and cancer risk.

Association of the four common polymorphisms in interleukin-10 (rs1800890, rs1800896, rs1800871, and rs1800872) with non-Hodgkin's lymphoma risk: a meta-analysis.

Interleukin-10 (IL-10) single nucleotide polymorphisms (SNPs) have been indicated to be correlated with Non-Hodgkin's lymphoma (NHL) susceptibility. However, the results of these studies on the association remain inconsistent. This meta-analysis was conducted to derive a more accuracy estimation of the association between the common SNPs (rs1800890, rs1800896, rs1800871 and rs1800872) in IL-10 and NHL risk.

PI3K/AKT signaling pathway plays a role in enhancement of eNOS activity by recombinant human angiotensin converting enzyme 2 in human umbilical vein endothelial cells.

The aim of this study was to investigate the effect of PI3K/AKT signaling pathway in the activity of recombinant human angiotensin converting enzyme 2 (rhACE2) promoted the activity of endothelial nitric oxide synthase (eNOS). The human umbilical vein endothelial cells (HUVEC) were cultured in vitro. Then treated with Ang II (1×10(-6) mol/L) for 24 h.

[A study on the development of TMPT-contained trial dentin sealant].

Purpose: To investigate the effect of resin curing system formulations of TMPT-contained trial dentin sealant on its bonding and polymerization properties.

Methods: Commercially available Hybrid Coat (HyC) and previously trial TMPT-contained dentin sealant T33 as control groups, adjusting camphorquinone (CQ) content to make CQ trial dentin sealants. Their dentin shear bond strength was tested to find the strongest group named CQn as the next test substrate.

Astrocytic expression of cannabinoid type 1 receptor in rat and human sclerotic hippocampi.

Cannabinoid type 1 receptor (CB1R), which is traditionally located on axon terminals, plays an important role in the pathology of epilepsy and neurodegenerative diseases by modulating synaptic transmission. Using the pilocarpine model of chronic spontaneous recurrent seizures, which mimics the main features of mesial temporal lobe epilepsy (TLE) with hippocampal sclerosis (HS) in humans, we examined the expression of CB1R in hippocampal astrocytes of epileptic rats. Furthermore, we also examined the expression of astrocytic CB1R in the resected hippocampi from patients with medically refractory mesial TLE.

Dual effects of ouabain on the regulation of proliferation and apoptosis in human umbilical vein endothelial cells: involvement of Na(+)-K(+)-ATPase α-subunits and NF-κB.

Purpose: To elucidate the effect of ouabain on the regulation of proliferation and apoptosis of HUVECs and involvement of different Na(+)-K(+)-ATPase α-subunits and NF-κB.

Methods: HUVECs were isolated by collagenase perfusion, and MTT assays and cell cycle analysis were performed to study proliferation. NF-κB expression and function were examined by immunohistochemical staining and western blotting.

[Recombinant hFOXA2 and hPDX1 lentivirus induced dental pulp stem cells from deciduous teeth reprogramming for insulin-producing cells].

Purpose: The purpose of this study was to culture and identify dental pulp stem cells(DPSCs) from deciduous teeth in vitro and construct the recombinant hFOXA2 and hPDX1 lentivirus vectors and transfect the DPSCs to induce insulin-producing cells (IPCs).

Methods: DPSCs were separated and cultured by enzyme digest method, and purified by limited dilution method. Flow cytometry was used to determine the surface marker expression of the DPSCs, and the ability of multiple differentiations was determined by specific staining.