Identification of Potential Intervention Targets Involved in Prior Exercise that Attenuates Peripheral Neuropathic Pain by Integrating Transcriptome and Whole-genome Bisulfite Sequencing Analyses.

Changes in DNA methylation and subsequent alterations in gene expression have opened a new direction in research related to the pathogenesis of peripheral neuropathic pain (PNP). This study aimed to reveal epigenetic perturbations underlying DNA methylation in the dorsal root ganglion (DRG) of rats with peripheral nerve injury in response to prior exercise and identify potential target genes involved. Male Sprague-Dawley rats were divided into three groups, namely, chronic constriction injury (CCI) of the sciatic nerve, CCI with prior 6-week swimming training (CCI_Ex), and sham operated (Sham).

Evaluating Whether Nonimmersion Virtual Reality Simulation Training Improves Nursing Competency in Isolation Wards: Randomized Controlled Trial.

Background: During infectious disease outbreaks such as the COVID-19 pandemic, nurses are crucial in patient care and public health safety; however, they face challenges such as inadequate training and high stress in isolation wards. Virtual reality (VR) technology offers innovative training solutions to enhance nurses' clinical skills and preparedness. However, extensive studies on its effectiveness in isolation ward environments are still limited.

Estimating cumulative infection rate of COVID-19 after adjusting the dynamic zero-COVID policy in China.

Background: At the end of 2022, China adjusted its coronavirus disease 2019 (COVID-19) prevention and control strategy. How this adjustment affected the cumulative infection rate is debated, and how second booster dose vaccination affected the pandemic remains unclear.

Methods: We collected COVID-19 case data for China's mainland from December 7, 2022, to January 7, 2023, reported by the World Health Organization.

ALOX15 Aggravates Metabolic Dysfunction-Associated Steatotic Liver Disease in Mice with Type 2 Diabetes via Activating the PPARγ/CD36 Axis.

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a prevalent hepatic disorder worldwide. Arachidonic acid 15-lipoxygenase (ALOX15), an enzyme catalyzing the peroxidation of polyunsaturated fatty acids, plays a crucial role in various diseases. Here, we sought to investigate the involvement of ALOX15 in MASLD.

Actions of dexmedetomidine in regulating NLRP3 in postoperative cognitive dysfunction in aged mice via the autophagy-lysosome pathway.

Background And Purpose: Autophagy-lysosomal pathway dysfunction leads to postoperative cognitive dysfunction (POCD). Dexmedetomidine (Dex) improves POCD, and we probed the effects of Dex on autophagy-lysosomal pathway dysfunction in a POCD model.

Experimental Approach: A POCD mouse model was established and intraperitoneally injected with Dex.

Will the increase of medical equipment affect medical expenditure? Empirical evidence from a quasi-experiment in China.

Background: Many studies have shown that using new technologies and medical equipment contributes to increasing health expenditure. Relatively less empirical studies have measured the impact of medical equipment on rising medical costs in China. Against this backdrop, we aim to examine whether the large-scale medical equipment deployment explains the increase in health expenditure.

Molybdate uptake interplay with ROS tolerance modulates bacterial pathogenesis.

The rare metal element molybdenum functions as a cofactor in molybdoenzymes that are essential to life in almost all living things. Molybdate can be captured by the periplasmic substrate-binding protein ModA of ModABC transport system in bacteria. We demonstrate that ModA plays crucial roles in growth, multiple metabolic pathways, and ROS tolerance in .

Splicing to orchestrate cell fate.

Alternative splicing (AS) plays a critical role in gene expression by generating protein diversity from single genes. This review provides an overview of the role of AS in regulating cell fate, focusing on its involvement in processes such as cell proliferation, differentiation, apoptosis, and tumorigenesis. We explore how AS influences the cell cycle, particularly its impact on key stages like G1, S, and G2/M.

Prediction Models for Postoperative Pneumonia in Elderly Hip Fracture Patients: A Systematic Review and Critical Appraisal.

Background: Although several models have been developed to predict postoperative pneumonia in elderly hip fracture patients, no systematic review of the model quality and clinical applicability has been reported.

Objective: To systematically review and critically appraise existing models for postoperative pneumonia in elderly hip fracture patients.

Design: Systematic review and meta-analysis.

Expert Consensus and Call on Actions for Weight Management in China: Advancing Healthy China Initiative Through Strategic Actions.

China faces a critical public health challenge with obesity rates exceeding 50% among adults and 20% among children. In response, the National Health Commission launched a comprehensive three-year "Year of Weight Management" initiative in March 2024, further emphasized by the 36th Patriotic Health Month's theme "Healthy Towns - Healthy Weight" in April 2024. These initiatives underscore the urgent necessity for implementing comprehensive strategies to combat obesity and its associated non-communicable diseases.

Mechanisms underlying EEG power changes during wakefulness in insomnia patients: a model-driven study.

Insomnia, as a common sleep disorder, is the most common complaints in medical practice affecting a large proportion of the population on a situational, recurrent or chronic basis. It has been demonstrated that, during wakefulness, patients with insomnia exhibit increased EEG power in theta, beta, and gamma band. However, the relevant mechanisms underlying such power changes are still lack of understanding.

Cryo-EM structure and complementary drug efflux activity of the Acinetobacter baumannii multidrug efflux pump AdeG.

Multidrug-resistant Acinetobacter baumannii has emerged as one of the most antibiotic-resistant bacterial pathogens associated with nosocomial infection, with its resistance highly depending on multiple multidrug efflux pumps. Here, we report the cryoelectron microscopy (cryo-EM) structure of Acinetobacter drug efflux G (AdeG), the inner membrane component of one of three important resistance-nodulation-cell division (RND) pump family members in A. baumannii, which is involved in drug resistance to chloramphenicol, trimethoprim, ciprofloxacin, and clindamycin.

Decomposing disparities in the utilization of basic public health services between locals and internal migrants in China: the role of social determinants.

Background: Internal migrants in China have long been at a disadvantage in terms of access to publicly financed services, as well as the utilization of public health services. The aim of the study was to examine inequities in the use of basic public health services between internal migrants and the local population and estimate the factors that contributed to inequity in use.

Methods: The data for this study was derived from the 2017 wave of the China Migrants Dynamic Survey.

Abnormal dynamic functional networks during pain-free periods: Resting-state co-activation pattern analysis in primary dysmenorrhea: Abnormal dynamic functional networks in primary dysmenorrhea.

Chronic pain alters the configuration of brain functional networks. Primary dysmenorrhea (PDM) is a form of chronic visceral pain, which has been identified spatial alterations in brain functional networks using static functional connectivity analysis methods. However, the dynamics alterations of brain functional networks during pain-free periovulation phase remain unclear.

Visual analysis of the research frontiers, hotspots and development trends of immunization programs for women and children.

The objective of this study is to gain insight into the current research frontiers, hotspots, and development trends in the field of immunization programs for women and children, and to provide scientific guidance and reference for follow-up research. Based on all the original research papers related to the research on immunization programs for women and children in the Web of Science Core Collection (WoSCC) database, bibliometric studies and visual analysis were carried out to explore the research frontiers, hotspots and development trends, and to analyze the risk factors affecting the vaccination coverage of immunization programs for women and children. Eight hundred forty-three papers obtained from 1,552 institutions in 96 countries/regions from January 1950 to August 2024, coauthored by 4,343 authors.

Integrating D-Dimer Thresholds into the Revised Caprini Risk Stratification to Predict Deep Vein Thrombosis Risk in Preoperative Knee Osteoarthritis Patients.

Introduction: Preoperative patients with knee osteoarthritis have a significantly increased risk of venous thromboembolism (VTE). While the Caprini risk assessment model offers some clinical guidance in predicting deep vein thrombosis (DVT), it has a relatively low predictive accuracy. Enhancing the model by integrating biomarkers, such as D-dimers, can potentially improve its accuracy.

Exploring artificial intelligence for differentiating early syphilis from other skin lesions: a pilot study.

Background: Early diagnosis of syphilis is vital for its effective control. This study aimed to develop an Artificial Intelligence (AI) diagnostic model based on radiomics technology to distinguish early syphilis from other clinical skin lesions.

Methods: The study collected 260 images of skin lesions caused by various skin infections, including 115 syphilis and 145 other infection types.

A new health literacy scale for staff in preschool childcare institution: development and preliminary validation.

Background: The health literacy of staff in preschool childcare institution is an important issue to consider in providing healthcare for children aged 3-6 years, which could contribute to reducing incidence of diseases and accidental injuries as well as maintaining children's good health. Seldom instruments have been designed to measure health literacy across this group. This research aims to develop a health literacy scale for staff in preschool childcare institutions and validate its psychometric properties.

Integrative Analysis of Pharmacology and Transcriptomics Predicts Resveratrol Will Ameliorate Microplastics-Induced Lung Damage by Targeting Ccl2 and Esr1.

Background: Microplastics (MPs) are ubiquitous on earth, posing a growing threat to human health. Previous studies have shown that the lung is a primary organ for MPs exposure. Resveratrol (RES) is a common dietary polyphenol that exhibits anti-inflammatory and antioxidant effects.

Curcumin-mediated antimicrobial photodynamic therapy for inactivating Mycobacterium abscessus: a promising approach for non-tuberculous mycobacterial skin infections.

Non-tuberculous mycobacterial skin infection lead to complex and lengthy treatment cycles. Antimicrobial photodynamic therapy (aPDT) is an emerging promising approach for treating infections. This study aims to assess the effects of aPDT using curcumin as a photosensitizer (PS) on non-tuberculous mycobacteria, Mycobacterium abscessus, a subtype that has become common in dermatology in recent years.

Metabolomics Revealed Cadmium Exposure Associated with Alterations in Serum Metabolism in Children.

Cadmium is a heavy metal contaminant known to cause various health issues. However, limited research exists on the serum metabolomic effects of cadmium exposure in children. In this study, we recruited 42 children to analyze their serum metabolomic profiles, along with measuring urinary cadmium and creatinine concentrations, to evaluate the impact of environmental cadmium exposure on serum metabolism.

The role of universal stress protein Usp1413 in meropenem adaptive resistance and environmental stress responses in .

Although various mechanisms of carbapenem-resistance have been identified in the nosocomial pathogen , the critical process of resistance evolution and the factors involved in are not well understood. Herein, we identified a universal stress protein Usp1413 which played an important role in adaptive resistance of to meropenem (MEM). Based on RNA-Seq and genome sequencing, Usp1413 was not only one of the most downregulated USPs, but also the bare one having mutation of tyrosine and glycine inserted at the site of 229-230 (YG229-230) under the stimulation of MEM.

Downregulation of HSP47 triggers ER stress-mediated apoptosis of hypertrophic chondrocytes contributing to T-2 toxin-induced cartilage damage.

T-2 toxin contamination in food and feed is a growing global concern, with its toxic effects on developing cartilage remaining poorly understood. In this study, we constructed an animal model using 4-week-old male Sprague-Dawley rats, which were administered T-2 toxin (200 ng/g body weight per day) by gavage for one month. Histological analysis showed a significant reduction in hypertrophic chondrocytes and increased caspase-3 expression and TUNEL staining in the deep cartilage zone of T-2 toxin-treated rats.

Nobiletin: a potential erythropoietin receptor activator protects renal cells against hypoxia.

Tangerine peel is a traditional Chinese herb and has been widely applied in foods and medicine for its multiple pharmacological effects. Erythropoietin receptor (EPOR), a member of the cytokine receptor family, is widely expressed in multiple tissues in especial kidney and plays protective effects in adverse physiological and pathological conditions. We hypothesized that it might be EPOR agonists existing in Tangerine peel bring such renal benefits.