Circulating miR-499 are novel and sensitive biomarker of acute myocardial infarction.

Background: miRNAs are known to play a crucial role in cardiac development, and the expression of miRNA is altered in the diseased heart. The aim of this study was to investigate the value of plasma microRNA-499 (miR-499) as a novel biomarker for early diagnosis of acute myocardial infarction (AMI).

Methods: Enrolled in this study were 227 patients with chest pain on presentation to the departments of emergency and cardiology of Wuxi Second People's Hospital between October 2011 and May 2014.

Multicenter comparison of the efficacy on prevention of pressure ulcer in postoperative patients between two types of pressure-relieving mattresses in China.

Objective: Present study is designed to evaluate the effects of preventing pressure ulcer in surgical patients with two types of pressure-relieving mattresses.

Methods: 1074 surgical patients from 12 hospitals in China were divided into A group (static air mattress with repositioning every 2 hours, n = 562) and B group (power pressure air mattress with repositioning every 2 hours, n = 512). The patient was subjected to a pressure-relieving mattress and observed from 0-5 days after surgery.

Differentiation of noncalculous periampullary obstruction: comparison of CT with negative-contrast CT cholangiopancreatography versus MRI with MR cholangiopancreatography.

Objectives: The purpose of this study was to compare CT with negative-contrast CT cholangiopancreatography (nCTCP) using subvolume minimum intensity projection (MinIP) versus MRI with MRCP in differentiating noncalculous periampullary obstruction.

Methods: Sixty-four patients with clinically proven noncalculous periampullary obstructions who had undergone both MDCT and MR examinations before operation were reviewed retrospectively. Two reviewers independently interpreted the two image sets (the CT with nCTCP set [CT set] vs.

Expression profile and clinical significance of microRNAs in papillary thyroid carcinoma.

This study screened microRNAs (miRNAs) that are abnormally expressed in papillary thyroid carcinoma (PTC) tissues to identify PTC and nodular goiter and the degree of PTC malignancy. A total of 51 thyroid tumor tissue specimens paired with adjacent normal thyroid tissues were obtained from the Department of Surgical Oncology of Hangzhou First People's Hospital from June-December 2011. miRNA expression profiles were examined by microarrays and validated by quantitative real-time PCR (qRT-PCR).

Abnormal platelet kinetics are detected before the occurrence of thrombocytopaenia in HBV-related liver disease.

Background & Aims: Thrombocytopaenia is a frequent feature in patients with HBV-related liver disease. Its underlying mechanism is not fully understood. Multiple factors might contribute to the development of thrombocytopaenia.

Comparative study of somatostatin-human serum albumin fusion proteins and natural somatostatin on receptor binding, internalization and activation.

Albumin fusion technology, the combination of small molecular proteins or peptides with human serum albumin (HSA), is an effective method for improving the medicinal values of natural small molecular proteins or peptides. However, comparative studies between HSA-fusion proteins or peptides and the parent small molecules in biological and molecular mechanisms are less reported. In this study, we examined the binding property of two novel somatostatin-HSA fusion proteins, (SST14)2-HSA and (SST28)2-HSA, to human SSTRs in stably expressing SSTR1-5 HEK 293 cells; observed the regulation of receptor internalization and internalized receptor recycling; and detected the receptors activation of HSA fusion proteins in stably expressing SSTR2- and SSTR3-EGFP cells.

Differential diagnosis of periampullary carcinomas: comparison of CT with negative-contrast CT cholangiopancreatography versus MRI with MR cholangiopancreatography.

Background: Negative-contrast CT cholangiopancreatography (nCTCP) has been introduced into clinical practice recently. In the present study, we compared CT with nCTCP vs. MRI with MR cholangiopancreatography (MRCP) for the differential diagnosis of periampullary carcinomas.

The expression and significance of T helper cell subsets and regulatory T cells CD₄⁺ CD₂₅⁺ in peripheral blood of patients with human leukocyte antigen B27-positive acute anterior uveitis.

Background: Human leukocyte antigen B27 (HLA-B27)-associated uveitis is the most common reason for non-infectious uveitis. This purpose of the research was to study the expression and significance of T lymphocyte subsets and CD₄⁺ CD₂₅⁺ T regulatory (Treg) cells in peripheral blood of patients with Human leukocyte antigen B27-positive acute anterior uveitis (HLA-B27-positive AAU).

Methods: The concentrations of Th1, Th2, Th17, CD₄⁺ CD₂₅⁺and CD₄⁺ CD₂₅⁺FOXP3⁺ Treg cells in peripheral blood were tested by flow cytometry.

MicroRNA-210 overexpression predicts poorer prognosis in glioma patients.

MicroRNA-210 (miR-210) levels are elevated in many tumor types, are frequently associated with hypoxia induction, and are correlated with poor prognosis in many solid tumors. miR-210 regulates cell growth, angiogenesis, invasion, and apoptosis of many human tumors. In this study, we investigated the clinical significance of miR-210 expression in common brain tumors, or human gliomas.

Regeneration of facial nerve defects with xenogeneic acellular nerve grafts in a rat model.

Background: Because of ease of harvest and low immunogenicity, xenogeneic acellular nerve graft (XANG) may be an alternative to autologous nerve to repair facial nerve defects.

Methods: Facial nerve defects of Wistar rats were repaired by XANG, and nerve gap regeneration was investigated by electrophysiological test, horseradish peroxidase (HRP) retrograde tracing and histomorphometric analysis, as compared to autograft.

Results: Twenty weeks after the grafting, electrophysiology showed that whisker pad muscles responded to the electrical stimuli given at the site proximal to the transplantation in 2 groups.

[Effects of H2 relaxin on the expression of Epac in a murine model of chronic asthma].

Objective: To explore the effects of H(2) relaxin on the expression of exchange protein directly activated by cyclic adenosine monophosphate (Epac) in a murine model of chronic asthma and the roles in the management of airway remodelling.

Methods: Thirty-two BALB/c mice were randomly divided into 4 groups of normal control, asthma, vehicle control and relaxin treatment (n = 8 each). They were sensitized and challenged with ovalbumin to establish a chronic asthmatic model.

[Study on treatment of 26 cases with nasal basal cell carcinoma].

Objective: To discuss the relationship between surgical margin and recurrence of nasal basal cell carcinoma.

Method: Twenty-six cases of nasal basal cell carcinoma were analyzed. Mohs microsurgical operation was used in 15 cases and conventional operation was used in 11 cases.

[Effects of two antigens for IgM detection of toxoplasmosis].

Objective: To observe the effects of recombinant Toxoplasma gondii P30 antigen and soluble antigen for IgM detection of toxoplasmosis.

Methods: Recombinant Toxoplasma gondii P30 antigen and soluble antigen were coated on two nitrocellulose membranes (NC) respectively. They were combined with the corresponding antibodies in the testing sera.

Improvement in imaging time and quality of 3D negative-contrast computed tomography cholangiography with minimum intensity projections: application of vari-slice manual cut and erosion functions.

Objective: To investigate the improvement in postprocessing time and image quality of 3D negative-contrast CT cholangiography (nCTC) with minimum intensity projection (minIP).

Methods: Thirty-eight patients with suspected biliary obstruction who underwent portal phase helical CT were retrospectively studied. Three-dimensional nCTC was generated using 3D tools with manual cut function in three methods: one-slice, two-slice, and vari-slice cut slab, respectively.

[Exploration of the auditory effects of diabetes mellitus using distortion product otoacoustic emissions].

Objective: To investigate the effects of diabetes mellitus on amplitude of distortion product otoacoustic emissions ( DPOAE) and whether streptozotocin has ototoxicity or not.

Methods: Twenty-four SD rats were divided into diabetes mellitus group, insulin therapy group and control group. Diabetes was induced by streptozotocin.

[A case report of injecting gentamicin intraocularly by mistake being misdiagnosed as central retinal artery occlusion].

Purpose: To report a case receiving gentamicin injection intraocularly by mistake when subconjunctival injection and was misdiagnosed as central retinal artery occlusion.

Methods: After a complete examination, including ocular fundus examination, fundus fluorescein angiography (FFA) and indocyanine green angiography (ICGA), the correct diagnosis was confirmed. The patient received a surgery of vitrectomy.

Identification of two novel mutations in Chinese patients with Dyschromatosis symmetrica hereditaria.

Dyschromatosis symmetrica hereditaria (DSH) is a rare autosomal dominant cutaneous disorder characterized by a mixture of hyperpigmented and hypopigmented macules of various sizes on the extremities. Pathogenic mutations in the DSRAD gene have recently been identified. In this study, we report and identify the mutations of the DSRAD gene in two Chinese pedigrees with DSH.

[Prevalence and drug tolerance of mycoplasma in patients with urogenital inflammation].

Objective: To observe the prevalence and drug tolerance of mycoplasma(Ureaplasma urealyticum and Mycoplasma hominis) in patients with urogenital inflammation.

Methods: Three thousand and fifty-five patients with urogenital inflammation such as non-gonococcal urethritis(NGU), chronic prostatitis or pelvic inflammation from 1999 to 2003 were included. The results of mycoplasma culture and drug sensitivity test were analyzed.

[Clinical significance of accumulated energy complex parameter in phacoemulsification].

Objective: The accumulated energy complex parameter [AECP, AECP = actual power (AP) x time (T)] was put forward and used to analyze the correlation between phacoemulsification energy and postoperative complication.

Methods: Ninety-two cases with senile cataract who underwent phacoemulsification were observed. The correlation between AECP and postoperative corneal edema rate (CER) was analyzed.