1 results match your criteria: "Wuhan University of Technology Wuhan 430070 China sunecho@whut.edu.cn h.xie@whut.edu.cn.[Affiliation]"
Synthesis of magnetic nanoparticles with an IDA or TED modified surface for purification and immobilization of poly-histidine tagged proteins.
RSC Adv
March 2020
School of Chemistry, Chemical Engineering, and Life Science, Wuhan University of Technology Wuhan 430070 China
Magnetic nanoparticles (MNPs) chelating with metal ions can specifically interact with poly-histidine peptides and facilitate immobilization and purification of proteins with poly-histidine tags. Fabrication of MNPs is generally complicated and time consuming. In this paper, we report the preparation of Ni(ii) ion chelated MNPs (Ni-MNPs) in two stages for protein immobilization and purification.
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